Biomedical subjects

Wei He

Publications and source records attributed to Wei He.

At least 127 records · Page 7Linked to original sources

PubMed2004

Telomerase activity of peripheral blood mononuclear cells from patients with laryngeal carcinoma.

This study investigated the activity of telomerase in peripheral blood mononuclear cells (PBMCs) in patients with laryngeal carcinoma. We examined proliferative response to phytohemagglutinin and expression of telomerase activity of PBMCs from 30 patients with laryngeal carcinoma and 17 healthy volunteers. Both proliferative and telomerase activity of [circled times]PBMCs in the patient group was lower than that in the healthy volunteer group. Telomerase activity may play a permissive role in cell division and clonal expansion of the immune cells in response to laryngeal squamous carcinoma. It may be related to lymph node metastasis of laryngeal cancer.

Aged↗

PubMed2004

[Protective effect of minocycline on oxygen/glucose deprivation and NMDA-induced neurotoxicity in rat primary neurons and hippocampal slices].

OBJECTIVE: To develop oxygen/glucose deprivation (OGD)-and NMDA-induced neurotoxicity models in rat primary neurons and hippocampal slices, and to determine the protective effect of minocycline. METHODS: The injuries of primary neurons were induced by OGD or NMDA (50micromol/L). Morphological changes of neurons were observed, and neuron viability was evaluated by MTT assay. The changes of light transmittance (LT) were induced by OGD or NMDA in rat hippocampal slices. The effects of minocycline and MK-801, an NMDA receptor antagonist, were observed in the models of OGD-or NMDA-induced injuries. RESULT: Minocycline concentration dependently inhibited OGD induced decrease of neuron viability and ameliorated neuron morphological changes at 1 and 10 micromol/L. It also inhibited NMDA insult at 10 and 100 micromol/L. MK-801 inhibited both injuries at 1 micromol/L. However, minocycline at 1 or 10 micromol/L did not inhibit the augment of LT in hippocampal slices induced by OGD or NMDA, while MK-801 inhibited both OGD-and NMDA-induced LT augments. CONCLUSION: Minocycline protects neurons from OGD insult, which may inhibit NMDA receptor-mediated neurotoxicity through an indirect pathway, but has no effect on OGD-or NMDA-induced immediate injury in hippocampal slices.

Animals↗

PubMed2004

[Study of correlation dimension on EEG].

The study of non-linear EEG is of great significance in clinical practice and research work. This paper has gone into the feasibility of calculating the correlation dimension and has developed some subjects with the characters of correlation dimension and the difference under four conditions: (1) passive eyes closed(PEC); (2) mental arithmetic with eyes closed(MAEC); (3) passive eyes open(PEO); (4) mental reasoning with eyes open (MRED). The results show it is feasible and meaningful to calculate correlation dimension and the correlation dimension can reflect the regular patterns of mental activity.

Algorithms↗

PubMed2004

[Cytotoxicity of MICA-reactive V delta 1 gamma delta T cells towards epithelial tumor cells].

OBJECTIVE: To confirm whether human MHC class I chain-related A (MICA) induces the amplification of V delta 1 gamma delta tumor-infiltrating lymphocytes (TILs) in vitro and to identify the cytotoxicity of MICA-reactive V delta 1 gamma delta TILs towards epithelial tumor cells. METHODS: MICA protein was prokaryoticly expressed and purified by molecular cloning technology. The purified recombined MICA (rMICA) was used to induce V delta 1 gamma delta T cells from tumor tissues in vitro and the cytotoxicity of these V delta 1 gamma delta TILs were tested by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT). RESULTS: The rMICA was expressed in prokaryocyte with pET30 as a vector. The immobilized rMICA protein could markedly induce the amplification of V delta 1 gamma delta T cells from tumor tissue in vitro. These V delta 1 gamma delta T cells showed strong cytolytic activities towards tumor cell lines expressing MICA. CONCLUSION: The MICA-reactive V delta 1 gamma delta T cell may be a candidate for adoptive cellular therapy of tumors.

Adult↗

PubMed2004

[Expression of macrophage colony stimulating factor in brains of PDAPPV717I transgenic mice].

OBJECTIVE: To identify the expression and distribution of macrophage colony stimulating factor (M-CSF) in brains of PDAPPV717I transgenic mice. METHODS: We detected the expression and distribution of M-CSF mRNA in brains of PDAPPV717I transgenic mice by using hybridization in situ and immunohistochemical staining. RESULTS: Expression of M-CSF mRNA was significantly higher in brains of PDAPPV717I transgenic mice than that in non-transgenic mice, and M-CSF mRNA in brain was mainly produced by reactive astrocytes. CONCLUSION: The results indicate that astrocytes play an important role in the onset/development of neuropathology of Alzheimer's disease.

Alzheimer Disease↗

PubMed2004

[Study on therapeutic window of opportunity for Panax notoginseng saponins following focal cerebral ischemia/reperfusion injury in rats].

OBJECTIVE: To study the therapeutic window of opportunity for Panax notoginseng saponins (Pns) following focal cerebral ischemia/reperfusion injury in rats. METHODS: Focal cerebral ischemia (2 h)/reperfusion (24 h) model in male rats was induced by transient occlusion and middle cerebral artery (MCA) for 2 h and reperfusion for 24 h. Drugs were administered at 3 h, 4 h, 5 h and 6 h after the onset of ischemia respectively and neurological deficit score, infarct size and brain edema were examined. RESULTS: The administration of Pns at 3-4 h after onset of ischemia significantly reduced neurological deficit score, infarct size and brain edema. When administrating Pns at 5 h after onset of ischemia, the neuroprotection decreased. When administrating Pns at 6 h after onset of ischemia, there are not significant protective effects. CONCLUSION: The therapeutic window of opportunity for Pns following focal cerebral ischemia/reperfusion injury in rats is not more than 5 h after the onset of ischemia.

Animals↗

PubMed2004

[Simulation of inverse recovery of epicardial potentials under incomplete boundary conditions].

Based on a 3-D inhomogeneous simulative body torso model, the influence of various boundary conditions on inverse recovered epicardial potential maps (EPM) was studied by boundary element method (BEM). The result shows that the precision of EPM calculated under incomplete boundary conditions will meet the clinical requirements, as long as the incomplete boundary conditions still contain the extremum area which often appears at the breast area.

Action Potentials↗

PubMed2004

Effects of ulinastatin on renal ischemia-reperfusion injury in rats.

AIM: To investigate the effect and possible mechanism of ulinastatin on renal ischemia-reperfusion injury in rats. METHODS: Male Sprague-Dawley rats were subjected to 45-min bilateral renal ischemia, treated with intravenously 12,500 U ulinastatin at 30 min prior to ischemia and at the beginning of reperfusion, compared with a nontreated group without ulinastatin and a sham-operation group without bilateral renal ischemia. After 0 h, 2 h, 6 h, 12 h, and 24 h of reperfusion, serum creatinine and blood urea nitrogen were measured for the assessment of renal function, renal sections were used for histologic grading of renal injury, for immunohistochemical localization of Bcl-2 and heat shock protein 70. Renal ultrastructure was observed through a transmission electron microscope. RESULTS: Ulinastatin significantly reduced the increase in blood urea nitrogen and creatinine produced by renal ischemia-reperfusion, suggesting an improvement in renal function. Ulinastatin reduced the histologic evidence of renal damage associated with ischemia-reperfusion and accompanied with an up-regulation in the expression of Bcl-2 protein, but it had no significant effect on the expression of HSP 70. Ulinastatin also significantly reduced kidney ultrastructure damage caused by renal ischemia-reperfusion. CONCLUSION: The protease inhibitor, ulinastatin, reduced the renal dysfunction and injury associated with ischemia-reperfusion of the kidney. The protective effect of ulinastatin might be associated with the up-regulation of Bcl-2 expression and the effect on membrane fragility.

Animals↗

PubMed2004

Effects of the Chinese drugs for activating blood circulation on plasma TXB2 and 6-keto-PGF1alpha contents in rabbits with glucocorticoid-induced femoral head necrosis.

In the femoral head necrosis model rabbit induced by endotoxin combined with methylprenisolone, the dynamical changes of plasma TXB2 and 6-keto-PGF1alpha contents were observed. As a result, microscopic examination showed that in the model group, bone trabeculae became fine, empty bone lacunae increased, and osteoblasts in number decreased; the ratio of TXB2 and 6-keto-PGF1alpha was out of balance, and these changes worsened gradually with the lapse of time; Compound Sheng Mai Cheng Gu Capsules could reverse femoral head necrosis, protect the vascular endothelial cells, recover the balance of TXB2 and 6-keto-PGF1alpha. It is concluded that the hormone-induced femoral head necrosis is closely related with blood stasis, and that the Chinese drugs for activating blood circulation and removing blood stasis can prevent the femoral head from development of necrosis.

6-Ketoprostaglandin F1 alpha↗

PubMed2004

[Study on relationship of biodegradable properties of PGLA film in vivo and in vitro].

This experimental study on the biodegradable properties of poly(glycolide-co-lactide)[PGLA] film in vivo and in vitro was aimed to investigate the relationship between in vivo degradation process and in vitro degradation process. First, PGLA film was cut to 1 cm x 1 cm in size. It was put into artificial saliva and PBS solution respectively in vitro, and was implanted into subcutaneous tissue in Wistar rat in vivo. Then the mass loss rate was calculated every week not only for sample in vitro but also in vivo. The molecular weight was measured every two weeks. The results showed that the degradable speed of PGLA was faster in artificial saliva than in PBS solution. The change of molecular weight was earlier than that of mass loss rate. The degradable period in vitro was about 9-10 weeks. The degradable period in vivo was about 8 weeks. The rate of in vivo degradation was 1.33 times faster than that of in vitro. In conclusion, the degradation of PGLA in vitro was mainly a chemical degradation process achieved by hydrolysis of ester bond. The degradation of PGLA in vivo would be affected by stress and by biological factors, thus the degradation process was apparently faster in vivo, but both accorded with the degradation kinetics model of aliphatic polyester. There was some relationship of biodegradation between in vitro and in vivo.

Absorbable Implants↗

PubMed2004

[An improved back-projection algorithm of dynamic electrical impedance tomography].

In this paper, an improved back-projection algorithm of dynamic electrical impedance tomography (EIT) was described. The improved back-projection algorithm modified the computing method of back-projection matrix B. Dynamic reconstruction image was obtained by the improved algorithm, and it was compared to the image obtained by the traditional method of equi-potential back-projection. The results showed that the improved algorithm of equi-potential back-projection could locate the object's position in the field with higher precision, and its speed was very fast. It also could improve the resolution of the reconstructed image in some extent.

Algorithms↗

PubMed2004

[Preliminary experimental study on treatment of portal hypertension with auxiliary partial orthotopic liver transplantation].

OBJECTIVE: To evaluate the therapeutic efficacy of auxiliary partial orthotopic liver transplantation (APOLT) on portal hypertension in liver cirrhosis with amelioration of portal vein congestion, changes in portal vein pressure and status of the graft. METHODS: The recipients were porcine model of biliary cirrhosis reproduced by ligation of the common bile duct. During transplantation, arterial blood pressure, central venous pressure were recorded. Buffer base, standard bicarbonate and pH of arterial blood samples were determined and analyzed in order to assess the impact of operation on the animals. The hemodynamics were also monitored. Color Doppler ultrasonographic examination was performed on recipients before operation, intra-operation and 7 days after operation, respectively. Portal vein pressure, blood bilirubin and variables of liver function were measured by using an autoanalyzer. Wedge biopsy specimens of each pig were obtained, stained with hematoxylin-eosin, and examined. Analysis of variance was performed. Otherwise non-parametric tests were used. RESULTS: Eight weeks after ligation of the common bile duct, biliary cirrhosis was reproduced in all the pigs, and histopathological examination of the liver specimen showed a large number of pseudo-lobules. In 6 pigs with hepatic cirrhosis liver transplantation was done. Five of the 6 (83.3%) animals survived for 7 days. One recipient died because of unsuccessful operation, the others showed stable hemodynamics during the operation. Seven days after transplantation, the blood flow of the two liver portal veins was observed by the use of color Doppler ultrasonography. It was found that the blood flow in the donor portal vein was much richer than that of native portal vein. The venous outflow of the graft was ample and smooth, and no thrombosis of the hepatic vein or portal vein was found. The variables, including alanine aminotransferase, aspartate aminotransferase, bilirubin and total bilirubin, were significantly improved 7 days after operation compared with pre-operative data. Portal pressure was found to be (20.76+/-2.42)cm H(2)O(1 cm H(2)O=0.098 kPa), (17.62+/-2.33)cm H(2)O and (14.72+/-2.25)cm H(2)O before the operation, during the operation, and 7 days after operation, respectively, and the difference was statistically significant(P<0.01). On the 7 days after transplantation, histopathological examination revealed evidence of damage with mild steatosis and sporadic necrotic hepatocytes and focal hepatic lobular degeneration in the graft, especially in the area around the central vein. CONCLUSION: APOLT is a hopeful option for the treatment of portal hypertension. This procedure can provide not only some improvement of the liver function but also decrease the pressure of portal vein.

Animals↗

PubMed2004

[Potent neutralization antibody elicited in mice by SARS-associated coronavirus spike protein S1 domain].

OBJECTIVE: To study the antigenicity of SARS associated coronavirus (CoV) spike S1 (12-672Aa) domain. METHODS: BALB/c mice were immunized with a plasmid bearing codon-optimized SARS-CoV (Tor2 strain) S1 domain and then boosted with purified S1 protein; the SARS-CoV specific IgG antibody was tested by ELISA and neutralization antibody was determined by in vitro microneutralization assay. RESULTS: S1 domain of SARS-CoV spike, which has been demonstrated harboring the receptor binding domain, successfully elicited SARS-CoV specific IgG antibody in mouse after combined immunization with DNA and purified S1 protein; the antibody elicited solely by S1 could potently neutralize SARS-CoV (HKU-39849) in vitro, 50% of 1 000 TCID50 SARS-CoV challenged cells were protected from viral infection by a 1:1499.68 dilution of mice sera immunized with S1 protein, but negative control sera showed no protection. CONCLUSION: S1 domain of SARS-CoV spike protein, which is responsible for receptor binding, can efficiently and sufficiently induce highly potent neutralizing antibody in mice. This result suggested that S1 domain could be an effective subunit vaccines against SARS-CoV.

Animals↗

PubMed2004

The protective immunity of a DNA vaccine encoding Schistosoma japonicum Chinese strain triose-phosphate isomerase in infected BALB/C mice.

The development of a DNA vaccine for schistosomiasis japonica and testing the protective efficacy after challenge in BALB/c mice were performed. Thirty-nine female BALB/c mice were divided into three groups. Each mouse of the control group was injected intramuscularly with 100 microg of pcDNA3.1 DNA. In the TPI group, each mouse was injected with 100 microg of pcDNA3.1-SjCTPI DNA. The TPI+IL-12 group was injected with 100 microg of pcDNA3.1-SjCTPI DNA and 100 microg of the mixture of pcDNA3.1-P35 and pcDNA3.1-P40 DNA. Each mouse was immunized three times at two-week intervals and challenged with 45 cercariae of Schistosoma japonicum Chinese strain four weeks post-immunization. Then the mice were sacrificed and perfused at 45 days after challenge; the recovered worms and hepatic eggs were counted. Cytotoxic T lymphocyte (CTL) activity mediated by SjCTPI was detected with the 51Cr release assay. ELISA was performed for the detection of anti-rTPI antibodies. Anti-rTPI antibody detection with ELISA after immunization showed ten serum samples from the control group were negative, five of ten serum samples from the TPI group were weakly positive, six of ten from the TPI+IL-12 group were also weakly positive. The CTL activity of the control group was 9.1%, while CTL activities of the TPI group and the TPI+IL-12 group were 27.6% and 54.4%, respectively. The worm and egg reduction rates of TPI group and the TPI+IL-12 group were 30.2%, 52.9%, 32.7%, and 47.0%, respectively in comparison with the control group. This study further proved the possibility of the SjCTPI DNA vaccine as a potential DNA vaccine for schistosomiasis.

Animals↗

PubMed2004

[Experimental study of inhibiting CTLL-2 cell apoptosis and enhancing cytotoxicity to Yac-1 cell by hammerhead ribozyme].

OBJECTIVE: To investigate the inhibition role of anti-Fas hammerhead ribozyme on Fas expression and Fas-mediated apoptosis in mouse cytotoxic T lymphocyte (CTL) cell line--CTLL-2 cells, and explore a novel approach to enhance the ability of T cells against leukemia in donor lymphocytes infusion (DLI). METHODS: A hammerhead ribozyme targeting the Fas mRNA was synthesized and transfected into CTLL-2 cells by electroporation. Fas expression in CTLL-2 cells was detected by using RT-PCR, Western blot and flow cytometry, CTLL-2 cells viability was measured by MTT assay, caspase-3 proteolytic activity by caspase-3 detection kit, and cell apoptosis by flow cytometry. Killing activity of CTLL-2 was detected by LDH releasing assay in vitro. RESULTS: Expression of Fas mRNA and protein in CTLL-2 cells was reduced to 50% after transfection with anti-Fas ribozyme. Being treated with anti-Fas antibody (JO(2)), compared with control and mock-transfected cells, viability of CTLL-2 cells transfected with anti-Fas ribozyme increased by 1-fold, caspase-3 activity and apoptosis rate of ribozyme-transfected cells decreased to 50% and 37%, respectively, and cell killing activity was enhanced by 2-fold. CONCLUSION: Anti-Fas ribozyme can cleave Fas efficiently and inhibit Fas-mediated apoptosis of CTLL-2 cells, resulting in improvement of their viability.

Animals↗

PubMed2004

[Plane scan analysis of the surface of white and blue porcelain by SRXRF method].

In this article, the authors analyze the surface of a piece of porcelain shred in Xuande Period by SRXRF, and the result shows that each peak area of elements differs in distribution pattern. According to the relationship between element peak area and color variation, and yellow fleck in glaze, it is possible to divide 13 elements, i.e. K, Cr, Mn, Fe, Co, Ni, Cu, Zn, Hg, Rb, Sr, Y and Zr, into three groups. This phenomenon will indicate how to search the "finger elements" in each dynasty; at the same time, it will present important information for research on the forming mechanism of yellow flecks in glaze.

English Abstract↗

PubMed2004

[Cloning and expression of tyrosinase-encoding gene (mel) of Bacillus thuringiensis and its initial research of application].

Tyrosinase, which is encoded by Tyrosinase gene (mel), is the key enzyme in the process of melanin formation in animals, plants and microorganisms. Using the primers designed by comparing the conserved domain of tyrosinase, a DNA fragment was amplified which contain the mel gene from Bacillus thuringiensis 4D11. The recombinant E. coli EMB1179 was gained by subcloning this DNA fragment onto the vector pGEM-7zf and transformed it into E. coli DH5alpha. EMB1179 express the tyrosinase activity and produce melanin under the presence of L-tyrosin. The effect of melanin on survival of E. coli was also determined. The results showed that the melanin produced by EMB1179 effectively increased its resistance against UV light.

Bacillus thuringiensis↗

PubMed2004

[Laparoscopic suture uterosacral ligament hysteropexy for uterine prolapse].

OBJECTIVE: To investigate possibility and effect of laparoscopic suture uterosacral ligament hysteropexy or colpopexy for women with uterine prolapse. METHODS: Thirty-two women with symptomatic uterine prolapse underwent laparoscopic suture uterosacral ligament hysteropexy. At the laparoscopic suture hysteropexy or colpopexy, the pouch of Douglas was closed and the uterosacral ligaments were plicated and reattached to the cervix. All patients were multipara and menopausal with prolapse of anterior wall of vagina. Additionally, 4 patients were with prolapse of posterior wall of vagina, 15 with stress urinary incontinence, and 4 with myomas. RESULTS: All procedures were successfully completed laparoscopically. The mean operating time for the laparoscopic suture hysteropexy or colpopexy alone was (32 +/- 11) min (range 20 approximately 80 min), and the mean blood loss was less than 50 ml. After a mean follow-up of (12 +/- 6) months (range 4 approximately 28 months), 23 women had no symptoms of uterine prolapse and seven had no objective evidence of uterine prolapse. Two women presented recurrence of uterine prolapse 3 months after operation. CONCLUSIONS: The laparoscopy suture hysteropexy or colpopexy is effective and safe in the management of symptomatic uterine prolapse. It may be an appropriate procedure for women with uterine prolapse hoping for uterine preservation.

Aged↗