Biomedical subjects

Wei-Bin Sun

Publications and source records attributed to Wei-Bin Sun.

7 recordsLinked to original sources

PubMed2006

[A new method of molecular marker--RMAPD].

Random microsatellite amplify polymorphic DNA (RMAPD) is a new method of molecular marker. It can reveal genome DNA polymorphisms of organism only when random primer and microsatellite forward or reverse primer are combined together as a pair of primers by the PCR reaction system with Taq DNA polymerase, MgCl2, dNTPs and contemplate DNA. The core question of RMAPD is validity of the primers used. A lot of experiments of RMAPD in 69 Xinong Saanen dairy goats showed that RMAPD primers were effective. Comparison of RMAPD, microsatellite and RAPD markers demonstrated that RMAPD was different from each other in primers, amplification protocol and repetition. RMAPD is not equal to RAPD, but a extendable RAPD. Therefore, RMAPD is regarded as a new method of molecular marker. As it has many characteristics of DNA marker, RMAPD has a widest potential application in animal breeding and genetics, such as analysis of genetic structure and relationship and MAS.

Animals↗

PubMed2006

[The biological changes of NIH3T3 cells co-cultured with human bone morphogenetic protein-2 gene transfecting cells].

OBJECTIVE: To investigate the ultrastructure and the alkaline phosphatase (ALP) activity changes of NIH3T3 cells incubated with secretive human bone morphogenetic protein-2 (hBMP-2) that is induced by gene transfection through transwell system. METHODS: Eukaryotic expression vector (pcDNA3.1-B2) was transduced into NIH3T3 cells by Sofast, a positive compound transfection agent. The positive cell clones were selected with G418. The cytoplasmic and extracellular expression of BMP-2 in the NIH3T3 cells were determined by immunohistochemical and enzyme-linked immunosorbent assay (ELISA). NIH3T3 cells were co-cultured with hBMP-2 gene transfecting cells through transwell system, and the ultrastructure and ALP activity (the markers of osteogenetic differentiation) changes were observed. RESULTS: There were cytoplasmic and extracellular expression of BMP-2 in transfecting NIH3T3 cells. The ultrastructure changes and the high expression of ALP suggested the osteogenetic differentiation tendency of NIH3T3 cells co-cultured with transfecting NIH3T3 cells. CONCLUSIONS: Secretive BMP-2 that is induced by gene transfection could promote the osteogenetic differentiation of fibroblast cells.

Alkaline Phosphatase↗

PubMed2005

[Study on mitochondrial DNA D-loop polymorphism in Chinese donkeys].

The mitochondrial DNA (mtDNA) D-loop sequences with 399 bp in 26 individuals from 5 donkey breeds in China were analyzed. Aligned by Clustal W software,the results showed that 23 polymorphic nucleotide sites and only transition with the percentage of 5.76% in 399 bp were observed. In reference to mtDNA D-loop sequences of European domestic donkey as a control, the average percentage of mtDNA D-loop nucleotide variation in 5 Chinese donkey breeds was 1.80%. The average percentages of D-loop nucleotide variation from Liangzhou donkey (LZ), Yunnan donkey (YN), Guanzhong donkey (GZ), Xinjiang donkey (XJ) and Jiami donkey (JM) were 0. 35%, 1.25%, 2.30%, 2.91% and 2.20% respectively. The average sequence divergence estimated from D-loop sequences varied from 0.25% - 5.01% within breeds and 4.51% - 5.51% among breeds, respectively, demonstrating that there existed rather abundant mitochondrial genetic diversity in Chinese donkeys. Comparisons of the 26 sequences revealed 11 mitochondrial haplotypes; the percentage of haplotype was 42.31%. This phenomenon demonstrated that the mitochondrial genetic diversity in Chinese donkey breeds is being reduced. It is urgent to protect the genetic resources of Chinese donkey. The molecular phylogenetic tree of mtDNA D-loop sequences in 5 Chinese donkey breeds,6 sequences of Asian wild ass (Equus asinus kiang, Equus asinus kulan, Equus asinus hemionus;) and 4 sequences of European domestic donkeys from GenBank was constructed by Neighbor-Joining method. It was the first time proved in molecular level that the origin of Chinese donkey breeds was from African wild ass (Equus africanus africanus and Equus africanus somaliensis), not from Asian wild ass as bescribed in the paper.

Animals↗

PubMed2005

[The effect of CSN1 S2, CSN3 and beta-lg genes on milk performance in Xinong Saanen dairy goat].

PCR-RFLP technique was applied to analyze correlation between the polymorphisms of CSN1 S2 (alpha(s2) casein), CSN3 (kappa casein) and beta-lg (beta-lactoglobulin) genes and milk performance in 69 individuals of Xinong Saanen dairy goat. The results showed that there was significant correlation between different genotypes of CSN1 S2 locus and milk yield:average milk yield of individuals with genotype FF was less than that of genotype NN (P < 0.05); the second lactation milk yield of individuals with genotype NN was over 90 kg higher than that with genotype FF (P < 0.01). This indicates that allele F of CSN1 S2 locus probably has significant negative effect on milk yield. The results of CSN3 gene digested with endonuclease Hind III cleavage showed that no significant difference of milk yield between genotype DE and genotype EE was detected in first, second, third and fourth lactation milk yield and average milk yield (P > 0.05). The results of CSN3 gene with endonuclease Taq I cleavage showed that no significant difference of milk yield among individuals with genotype TT, TC and CC was detected (P > 0.05). No polymorphism was detected in PCR products of CSN3 gene digested with endonuclease Hae III. The analysis of beta-lg gene's 5' flanking region (710 bp) by PCR-RFLP in Xinong Saanen dairy goat showed that milk yield of individuals with genotype AA was higher than that with genotype AB in second, third lactation milk yield and average milk yield (P < 0.05). The results implied that allele A of beta-lg gene's 5' flanking region is probably related to high milk yield.

5' Flanking Region↗

PubMed2005

[Research on the genetic polymorphism in crested ibis by RMAPD].

The genetic polymorphism of Yangxian artificially reared 43 crested ibises (Nipponia nippon) was firstly investigated by RMAPD (random microsatellite amplify polymorphic DNA) marker. The results showed that RMAPD was more stable and polymorphic than RAPD. 2147 bands were amplified by 12 pairs of primers,of which 93 bands had polymorphisms. The band sharing frequency and the genetic diversity index were 0.718 and 3.664, respectively, indicating that the genetic structure of crested ibis population was simple and poor genetic variations existed in crested ibis population in Yangxian. It is imperious to protect the genetic diversity of crested ibis population.

Animals↗

PubMed2004

[Study on mitochondrial DNA genetic diversity of some cattle breeds in China].

The complete mitochondrial D-loop sequences, 910 bp in length, in 22 individuals from 8 cattle breeds in China were analyzed. The results showed that A% + T% was about 61.65%. Comparisons of these 22 sequences revealed 66 polymorphic sites, and 5 types of mutation, transition, transversion, insertion, deletion and coexistence site of transition and transversion were observed,with the percentage of 81.82%, 6.06%, 7.57%, 3.03%, 1.52%, respectively. In reference to complete mtDNA D-loop of the European cattle as a control, eight Chinese cattle breeds were classified into 3 groups according to the average percentage of D-loop nucleotide variations. The lowest average percentage of mtDNA D-loop nucleotide variation was in Xizhen cattle, Mongolian cattle, Holstein, Qinchuan cattle with 0.37%, 0.44%, 0.52%, 0.66%, the mediate in Nanyang cattle and Jiaxian cattle with 1.91%, 2.02% and the highest in Jinnan cattle and Yueyang cattle with 4.47%, 4.73%, respectively. The average sequence divergence estimated from D-loop region within breeds and among breeds in China varied from 0.55% - 5.39% and 1.21% - 6.59%, respectively. Comparisons of these 22 sequences revealed 19 mitochondrial haplotypes, the percentage of haplotype was 86.36%, showing that abundant mitochondrial genetic diversity exists in Chinese cattle. The molecular phylogenetic tree of mtDNA D-loop of 8 Chinese cattle breeds was constructed by Neighbor-Joining method. The NJ tree indicated that these mtDNA sequences fell into 3 distinct haplotype groups, it also suggested in molecular level that there were probably 3 maternal origins, of which the main origins of Chinese cattle were from Bos taurus and Bos indicus.

Animals↗

PubMed2003

[Polymorphisms of insulin-like growth factor binding protein 3 gene and its associations with several carcass traits in Qinchuan cattle].

DNA samples from 60 Qinchuan cattle (Bos taurus) were analyzed with PCR-RFLPs and sequencing for insulin-like growth factor binding protein 3 (IGFBP3) gene. Fragments of 651 bp were amplified with two primers and the products of PCR were digested with restriction endonuclease HaeIII. The produced fragments showed three genotypes, namely AA,AB and BB after electrophoresis. Frequencies of the genotype AA,AB,BB and allele A,B were 0.7,0.28,0.02,and 0.84,0.16,respectively. Sequence analysis showed that a transversion of C-->A at 299 nt resulted in loss of the cleaved site of restriction endonuclease HaeIII and produced this polymorphism. This polymorphic locus of IGFBP3 gene was at Hardy-Weinberg equilibrium (P>0.05). The genotypes of AA,AB,BB slightly affected several slaughter and carcass traits of Qinchuan cattle. Dressing percentage,net meat percentage, striplion percentage, tenderloin percentage, ribeye percentage and tender shoulder percentage were decreased with the genotypes of AA,AB and BB in Qinchuan cattle, but it was not significant (P>0.05). Average ribeye area in individuals of AA genotype was significantly higher than that in individuals of BB genotype (P<0.05), and beef fat content in individuals of genotype AB and BB was significantly higher than that in individuals of AA genotype (P<0.01).

English Abstract↗