Biomedical subjects

Wei-Cho Huang

Publications and source records attributed to Wei-Cho Huang.

4 recordsLinked to original sources

PubMed2006

Microfluidic enzyme-linked immunosorbent assay technology.

In this chapter, we have presented an overview of microfluidic enzyme-linked immunosorbent assay (ELISA) by first introducing the principle of immunoassay, ELISA, and microfabricated devices, followed by a discussion of microfabrication technology and the characterization of microfluidic components. Significant advances in laboratory technology are contributing to the further understanding of microfluidic function, surface modification and immobilization, which lead to the development of improved biomolecule detection methods and prospective applications. For the future, the exploitation of more robust-manufacturing processes and integrated assay systems in an automatic fashion with much reduced assay time and reagent consumption will allow for the effective detection and quantification of biological agents that are of interest in medical diagnostics, food safety surveillance, and environmental monitoring.

Enzyme-Linked Immunosorbent Assay↗

PubMed2004

Continuous production of butanol by Clostridium acetobutylicum immobilized in a fibrous bed bioreactor.

We explored the influence of dilution rate and pH in continuous cultures of Clostridium acetobutylicum. A 200-mL fibrous bed bioreactor was used to produce high cell density and butyrate concentrations at pH 5.4 and 35degreesC. By feeding glucose and butyrate as a cosubstrate, the fermentation was maintained in the solventogenesis phase, and the optimal butanol productivity of 4.6 g/(L h) and a yield of 0.42 g/g were obtained at a dilution rate of 0.9 h-1 and pH 4.3. Compared to the conventional acetone-butanol-ethanol fermentation, the new fermentation process greatly improved butanol yield, making butanol production from corn an attractive alternative to ethanol fermentation.

Acetone↗

PubMed2003

Effects of pressure pulsation on oxygen transfer rate measured by sulfite method.

Pressure pulsation (PP) was investigated for its effects on oxygen transfer rate (OTR) measured by sulfite oxidation. By manipulating airflow rate, 0.41- 1.2 vvm, and a control valve in a 4-L bioreactor, the frequency of PP was varied at different gas pressures3-15 psig. A mathematical model of OTR was built and compared to experimental data. OTR was also examined at constant gas pressure, 4.5-15.0 psig. The results indicate a good agreement between measurement and model prediction. OTR above 9 psig during PP showed significant enhancement at 25 degrees C. This proves that PP not only affects the elevation of DO level, but also increases the interfacial area and mass transfer coefficient.

Aerobiosis↗

PubMed2002

Enhancement of oxygen transfer by pressure pulsation in aqueous glycerol fermentation.

Aeration plays an important role in the production of glycerol by fermentation with yeast. Effective aeration depends on a number of factors, such as amount of air, fineness of air dispersion, rate of agitation, and time of gas-liquid contact. This investigation dealt with the effect of periodic variation in gas pressure on oxygen transfer measured by sulfite oxidation and glycerol fermentation in stirred tanks. The oxygen transfer rate measured with the sulfite oxidation method was improved by 20-30% under the condition of pressure pulsation (PP) at 30 degrees C. The yield and productivity of glycerol were increased by about 26 and 6.8%, respectively, in 48 h by employing a glucose concentration of 250 g/L with PP at 30 degrees C.

Aerobiosis↗