Biomedical subjects

Wei-Li Yan

Publications and source records attributed to Wei-Li Yan.

3 recordsLinked to original sources

PubMed2006

Common SNPs of APM1 gene are not associated with hypertension or obesity in Chinese population.

OBJECTIVE: To investigate whether the common variants 45T/G and 276G/T in APM1 gene were associated with hypertension combined with obesity (HO) and related clinical features in Chinese Han population. METHODS: A case-control study design was applied. Common polymorphisms of 45T/G and 276G/T were genotyped by PCR product sequencing in 484 cases with HO and 502 controls with normal blood presure and BMI < 25. RESULTS: The genotype and allele frequencies of 45T/G, 276G/T, and haplotype defined by the two variants in cases did not differ from those in controls. The means of blood pressure, BMI and waist-hip ratio did not differ among genotypes of the two polymorphisms and haplotypes. Among lipid profiles, only serum high-density lipoprotein cholesterol (HDL-C) levels were significantly lower in T allele carriers than that in non-T carriers after adjusting possible confounding factors (1.21 vs 1.32 mmol/L, P=0.0001). CONCLUSION: Polymorphisms of 45T/G and 276G/T in APM1 gene are not associated with hypertension or obesity, or their clinical features in Chinese Han population. Common polymorphism of 45T/G might be associated with serum HDL-C levels in Chinese.

Adiponectin↗

PubMed2005

L-[1-13C] phenylalanine breath test results reflect the activity of phenylalanine hydroxylase in carbon tetrachloride acute injured rat liver.

L-[1-13C] phenylalanine breath tests (PheBTs) have been used to determine the hepatocyte functional capacity of patients. This study investigated the relationship between the PheBT parameter 13C excretion rate constant (PheBT-k) and activity of the phenylalanine metabolic rate-limiting enzyme phenylalanine hydroxylase (PAH) in rat liver. We noted that the time-course curves of 13C excretion presented as a single peak, which appeared 2 min after administration of L-[1-13C] phenylalanine (13C-Phe). 13C excretion during exhalation can be divided into a slow phase and a rapid phase. The PheBT-k in rats with carbon tetrachloride acute liver injury was.significantly lower than that of control rats. The rapid phase 13C disposition constants of the acute liver injured rats did not differ from that of the controls. The peak value of 13C abundance in the breath of the acute liver injured rats was markedly higher than that of the control group. Total liver PAH activity in the acute liver injured rats was significantly lower than that in the control group. PheBT-k was highly correlated with the total activity of liver PAH (r = 0.92, P < 0.001). The present findings indicate that PheBT results reflect PAH activity levels. The PheBT-k parameter is a sensitive index that can be used to evaluate PAH function in the liver. In addition we demonstrated that the rodent model used in this study is a valuable tool for basic research studies of the breath test.

Acute Disease↗

PubMed2004

[Issues on association studies on complex diseases].

Association study is widely used in elucidating genetic basis of complex diseases such as cardiovascular diseases, type 2 diabetes, essential hypertension and obesity. Some issues were discussed in the review, which include defining and controlling of confounding effects, selection of candidate genes and single nucleotide polymorphisms (SNPs), application of intermediate phenotype and haplotype analyses as well as judgement of the result in association studies. Population stratification is one of the major causes of confounding in association studies. It could be reduced by selecting relatives of affected patients as control, genome control and by using isolated populations which have higher homogeneity in genetic background. A candidate gene could be selected because of its' biological association with some disease or being congenerous to a known gene related to the disease. Appropriate application of intermediate phenotype of diseases and haplotype analyses may increase the opportunity to obtain meaningful findings in association studies. An optimal study design, sufficient sample size and proper controls, in conjunction of modern statistic analyses, association analyses would exert its effect on studies on susceptibility of human common diseases.

Cardiovascular Diseases↗