Biomedical subjects

Wei-Min Yang

Publications and source records attributed to Wei-Min Yang.

7 recordsLinked to original sources

PubMed2006

LRIG1, a candidate tumour-suppressor gene in human bladder cancer cell line BIU87.

OBJECTIVES: To determine the effects of LRIG1 on the growth, migration and invasion of bladder cancer cells and the mechanisms underlying such effects. MATERIALS AND METHODS: The plasmid pLRIG1-green fluorescence protein (GFP) was transfected into BIU87 bladder cancer cells by Lipofectamine2000 (Invitrogen, Groningen, the Netherlands), and the cells that expressed LRIG1 stably were screened out by G418. The changes in LRIG1 and epidermal growth factor receptor (EGFR) protein levels were measured by Western blot; growth curves were estimated by the tetrazolium (MTT) assay; then cell-cell adhesion, cell-matrix adhesion and cell invasion assays were used to measure proliferation, adhesion and invasion in LGIR1-transfected and control cells. RESULTS: The LRIG1 protein level in pLRIG1-GFP transfected cells was significantly higher than that in control cells, while the EGFR protein level was significantly lower. pLRIG1-GFP transfected cells had less proliferation than control cells. Contrasting with non-LRIG1-transfected cells, the invasion and cell-matrix adhesion ability of pLRIG1-GFP transfected cells decreased markedly, and conversely the homotypic cell-cell adhesion ability was significantly higher. CONCLUSIONS: LRIG1 might act as a tumour-suppressor gene, participating in negative feedback control of EGFR expression, which inhibits bladder cancer cells from growth, migration and invasion.

Blotting, Western↗

PubMed2006

[Effects of PTEN gene transfection on proliferation and invasion of human bladder cancer cell line BIU87].

BACKGROUND & OBJECTIVE: PTEN (phosphatase and tensin homologue deleted from chromosome 10) is the first antioncogene with phosphatase activity till now, and was found absent or mutational in many primary malignant tumors and cell lines. Its inactivation is correlated to tumor development and prognosis. This study was to investigate the effects of PTEN transfection on proliferation and invasion of human bladder cancer cell line BIU87. METHODS: A eukaryotic expression plasmid containing PTEN, pBp-PTEN, was introduced into E. coli DH5alpha and amplified. The plasmid was prepared and purified, and then identified by restrictive enzyme digestion. pBp-PTEN was transfected into BIU87 cells, and positive cell clones (pBp-PTEN-BIU87) were selected and amplified. Empty plasmid-transfected BIU87 cells (pBp-BIU87) and normal BIU87 cells were set as control. The expression of PTEN was detected by reverse transcription-polymerase chain reaction (RT-PCR). Proliferation and invasion ability of BIU87 cells were measured before and after transfection by MTT assay and cell invasion assay. RESULTS: Plasmid pBp-PTEN containing PTEN was successfully constructed and transfected into BIU87 cells. After transfection, the inhibitory rates of cell growth at the first, second, third, and fourth days were 4.27%, 18.92%, 19.54%, and 17.69%, respectively. The penetrating cells were significantly less in pBp-PTEN-BIU87 group than in pBp-BIU87 and BIU87 groups (39.3+/-7.7 vs. 48.1+/-13.2 and 48.9+/-11.0, P<0.05). CONCLUSION: Transfection with PTEN might suppress proliferation and invasive ability of bladder cancer BIU87 cells.

Cell Line, Tumor↗

PubMed2005

[Comparison between two methods for staining DNA of apoptotic spermatozoa].

OBJECTIVE: To compare two fluorochrome staining methods for the assessment of sperm quality. METHODS: Washed sperm cells were incubated in 0, 0.15, or 15 micromol/L camptothecin (CAM), or 0.37 or 3.7 mmol/L genistein (GEN) at 37 degrees C for 4 hours. The sperm cells were analyzed for cycle-independent apoptosis and necrosis by single-stain compared with dual-stain fluorescence microscopy to contrast the relative effectiveness of these two approaches. RESULTS: The single-stain procedure could not detect the sperm viability differences. In contrast, the dual-stain procedure identified a dosage-dependent decrease in the viability and increased necrozoospermia after topoisomerase inhibitor CAM and GEN treatments. Apoptosis was 2-fold higher with topoisomerase inhibitor treatment. CONCLUSION: The two topoisomerase inhibitors were associated with increased apoptosis and dosage-dependent necrosis. The data suggested that the dual-stain combination Hoechst 33342/PI was more sensitive than the single Hoechst 33342 stain analysis and permitted quantitative analysis of the apoptosis and necrosis in sperm.

Apoptosis↗

PubMed2005

[Correlation between abnormal serum lipid and erectile dysfunction].

OBJECTIVE: To achieve the correlation between abnormal serum lipid and male erectile dysfunction (ED). METHODS: Randomly selected were 200 male patients abnormal at least in one of the four laboratory items: total cholesterol (TC), triglycerides (TG), high density lipoprotein (HDL) and low density lipoprotein (LDL). Their erectile function was evaluated by IIEF-5, and the correlation was studied between abnormal serum lipid and ED. RESULTS: The incidence of ED was 47% in these patients. Age, coronary heart disease (CHD), high serum glucose, benign prostatic hyperplasia (BPH), drug, hypertension were negatively, while HDL was positively correlated with erectile function. Age, CHD, BPH and TC/HDL ratio were risk factors of ED, while HDL was a preventive one. CONCLUSION: Hyperlipidemia is an important factor of ED. HDL and TC/HDL ratio are good predictors of ED.

Adult↗

PubMed2005

[Microcirculation changes in the ventral prostates of rats after castration].

OBJECTIVE: To study the microcirculation changes in the ventral prostates of rats after castration and the role of microcirculation during the apoptosis of prostatic cells. METHODS: Thirty-six male adult rats were randomized to 6 groups: one was taken as the control, while the other 5 underwent measurement of the microcirculation in vivo by a D95 physiological signal acquisition system 12 h, 24 h, 72 h, 7 d and 14 d respectively after castration, and then were perfused with Chinese ink to trace the microvessels of the prostates. RESULTS: The microcirculation of the rats'prostates changed dramatically following castration. The diameter and density of the microvessels, especially in the distant and mediate ducts of the prostates, decreased dramatically, and so did the bloodflow velocity. CONCLUSION: The microcirculation plays a role during the process of apoptosis of prostatic cells, and might be the mechanism of " apoptosis shift".

Animals↗

PubMed2003

Albaconol from the mushroom Albatrellus confluens induces contraction and desensitization in guinea pig trachea.

The contraction and desensitization induced by albaconol and the influence of capsazepine, capsaicin and extracellular Ca2+ were investigated to see whether the actions were mediated via a specific VR receptor in guinea pig trachea spiral strips in vitro. Both albaconol and capsaicin were contractors of tracheal smooth muscle, but albaconol was not so potent as capsaicin, with -log (M) EC50 values of 4.23 +/- 0.18 (n = 10) and 7.33 +/- 0.21 (n = 10) respectively. 2.5 microM capsazepine competitively antagonized the contractile response to albaconol and capsaicin. Albaconol increased the contraction induced by a low dose of capsaicin (10(-10) to 10(-9) M), but non-competitively antagonized the contraction induced by a high dose of capsaicin (10(-8) to 10(-3) M). Either albaconol (1 or 100 mM) or capsaicin (3 or 10 microM) was able to desensitize the isolated guinea pig bronchi to subsequent addition of albaconol. Capsazepine (5.0 microM) significantly prevented the desensitization induced by either albaconol (1 or 100mM) or capsaicin (3 or 10 microM). Extracellular Ca2+ was essential for albaconol to induce excitation, but it did not affect albaconol- or capsaicin-induced desensitization. In summary, the results from the present study suggest that albaconol induces contraction and desensitization of guinea pig trachea in vitro as a partial agonist for VR.

Agaricales↗

PubMedDate not supplied

Antioxidant activities of three dihydrochalcone glucosides from leaves of Lithocarpus pachyphyllus.

In vitro antioxidant activities of three sweet dihydrochalcone glucosides from the leaves of Lithocarpus pachyphyllus (Kurz) Rehd. (Fagaceae), trilobatin 2"-acetate (1), phloridzin (2) and trilobatin (3), were investigated. The IC50 (50% inhibitory concentration) values for compounds 1-3 of lipid peroxidation in rat liver homogenate were 261, 28, 88 microM, respectively. Compounds 1-3 increased superoxide dismutase (SOD) activity with EC50 (50% effective concentration) values of 575, 167, 128 microM, and glutathione peroxidase (GSH-Px) activity with EC50 values of 717, 347, 129 microM, respectively, and showed only weak DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity.

Animals↗