Biomedical subjects

Weili Yan

Publications and source records attributed to Weili Yan.

3 recordsLinked to original sources

PubMed2003

Biomimetic synthesis of gramicidin s and analogues by enzymatic cyclization of linear precursors on solid support.

[reaction: see text] Gramicidin S is a potent decapeptide antibiotic with high hemolytic activity but is unlikely to provoke microbial resistance. Here we demonstrate that gramicidin thioesterase (GrsB TE) correctly cyclizes immobilized linear decapeptide precursors into head-to-tail products, indicating its suitability for parallel solid-phase synthesis of gramicidin analogues from linear precursors on solid support. This chemoenzymatic method will enable the optimization of the therapeutic index of the natural product to fight microbial resistance.

Anti-Bacterial Agents↗

PubMed2003

The application of the generalized vector sample pattern matching method for EIT image reconstruction.

This paper presents a new application of a generalized vector sample pattern matching (GVSPM) method for image reconstruction of conductivity changes in electrical impedance tomography. GVSPM is an iterative method for linear inverse problems. The key concept of the GVSPM is that the objective function is defined in terms of an angular component between the inner product of the known vector and solution of a system of equations. Comparisons are presented between images of simulated and experimental data, reconstructed using truncated singular value decomposition and GVSPM. In both cases, a normalized sensitivity matrix is constructed using the finite volume method to solve the forward problem.

Artifacts↗

PubMed2002

Substrate spectrum of tyrocidine thioesterase probed with randomized peptide N-acetylcysteamine thioesters.

Apparent kinetic constants k(cat) and K(m) were determined for tyrocidine thioesterase (TycC TE) using randomized peptide N-acetylcysteamine thioesters as substrate analogues. The enzyme has been found to be adequately active for the synthesis of positional-scanning libraries for novel antibiotic screening with reduced k(cat)/K(m) in the range of 2 to 82 folds lower than that of the wild-type sequence

Acetylcysteine↗