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Wen-zhong Liu

Publications and source records attributed to Wen-zhong Liu.

9 recordsLinked to original sources
PubMed2006

[Correlation of C-reactive protein with activity of Crohn's disease].

OBJECTIVE: To investigate the role of C-reactive protein (CRP) in evaluating activity of Crohn's disease (CD). METHODS: 85 patients with Crohn's disease, 59 males and 26 females, aged 15-69, underwent laboratory examination of CRP, erythrocyte sedimentation rate (ESR), albumin, and hemoglobin. Crohn's disease activity index (CDAI) was calculated. Endoscopy was conducted to collect samples of colon mucosa to undergo HE staining for pathological examination and immunohistochemical staining for the expression of nuclear factor-kappaB p65 (NF-kB p65). Logistic regression analysis was used to estimate the correlation of CRP with ESR, CDAI, endoscopic activity, pathologic activity, hypoalbuminemia, and anemia. The effects of lesion location, clinical severity, and drug were considered. RESULTS: CRP elevation was correlated with ESR, but not correlated with CDAI, endoscopic activity, pathologic activity, hypoalbuminemia, and anemia. CRP was significantly elevated in active CD (P < 0.01), especially in severe or colonic CD (P < 0.05). CRP rapidly decreased when the CD activity was effectively controlled by drugs (P < 0.01), and was reelevated when disease recurred (P > 0.05). Serum CRP elevation was paralleled with the NF-kappaB p65 level (P = 1.0). CONCLUSION: More suitable for reflection of the activity of moderate or severe colonic CD, CRP can reflect the effects of drugs in the early stage of CD. The serum CRP elevation is consistent with the NF-kappaB p65 expression in colon tissues.

Adolescent↗
PubMed2006

[Effects of Helicobacter pylori infection on neural expression of stomach and spinal cord: an experiment with mice].

OBJECTIVE: To investigate the effects of Helicobacter pylori (Hp) infection on neural expression in stomach and spinal cord, and to investigate the mechanism of functional dyspepsia after Hp infection. METHODS: Thirty-five female C57BL/6 mice were randomly divided into three groups: Group A (acute infection group, undergoing intragastric gavage of Hp suspension every other day for 3 times and then observed for 2 weeks, 15 mice), Group B (chronic infection group, undergoing intragastric gavage of Hp suspension every other day for 3 times and then observed for 2 weeks, 15 mice) and control group (undergoing intragastric gavage of normal saline every other day for 3 times and then observed for 2 weeks, 5 mice). After the observation the mice were killed and their stomachs were taken out to undergo gastric histology and bacterial colonization by HE staining and Warthin-Starry staining respectively. Their spinal cords of thoracic and lumbar segments were taken out too. Immunohistochemistry was used to detect the expression of Fos, vasoactive intestinal polypeptide (VIP), and calcitonin gene-related peptide (CGRP) in the stomach and spinal cord. RESULTS: Three mice died 12 weeks after Hp infection. The rate of Hp colonization, mainly localized in pyloric gland region, was greater in Group B than in Group A, and was 0 in the control group. The severity of inflammation as shown by mononuclear cell infiltration, and activity of inflammation as shown by polymorphonuclear cell infiltration, in the pyloric gland region, proventriculus-glandular stomach region, and corpus gland region were more pronounced in Groups A and B, especially in Group B, than in the control group. The expression values of Fos, VIP, and CGRP in the stomach of Group A were 3.1 +/- 1.4, 4.5 +/- 1.8, and 2.4 +/- 0.8 respectively, all not significantly different from those of Group B (3.1 +/- 1.3, 3.5 +/- 1.6, and 2.2 +/- 0.8, all P > 0.05). The expression values of Fos, VIP, and CGRP in the spinal cord of Group A were 3.8 +/- 1.2, 3.2 +/- 1.5, and 2.2 +/- 0.6, all not significantly different from those of Group B (3.4 +/- 0.7, 2.6 +/- 1.2, and 2.5 +/- 1.1, all P > 0.05 for all). However, the neural expression values in both acute and chronic infection groups were significantly higher than those in the control group (2.4 +/- 0.9, 1.6 +/- 0.9, and 1.2 +/- 0.8 in stomach; and 2.0 +/- 1.6, 1.2 +/- 1.1, and 1.2 +/- 1.1 in spinal cord, P < 0.05 for all). CONCLUSION: Hp infection, both acute and chronic, induces gastric histological changes such as inflammation and activity, and enhances the Fos, VIP, and CGRP expression in stomach and spinal cord, which can be a basis for symptom generation in dyspeptic patients with Hp infection.

Animals↗
PubMed2006

[Histopathological study on the regularity of pyriform sinus carcinoma invading adjacent tissue and structure].

OBJECTIVE: To explore the regularity invading adjacent tissue of pyriform sinus carcinoma. METHODS: The whole organ serial section of 68 total or partial laryngectomy and hypopharyngectomy specimen of pyriform sinus carcinoma were histopathologically studied. RESULTS: In 68 pyriform sinus carcinoma, invaded ventricular and paraglottic spaces was 63 and 38 cases respectively, the difference of invasive frequency of both spaces was significantly marked (chi2 = 21.37, P < 0.01). Thyroid cartilage had the most invaded frequency of 92.6% (63/68). The all touching and pressing invasion of laryngeal cartilage was 89 times, and infiltrating invasion was 51 times. The invasive frequency of lateral cricoarytenoid muscle, posterior cricoarytenoid one, thyroarytenoid and interarytenoid ones were 63.2% (43/68), 57.4% (39/68), 55.9% (38/68), 51.5% (35/68) respectively. The invasive frequency of cricoarytenoid and cricothyroid joints were 30.9% (21/68), 17.6% (12/68) respectively. The invasive frequency of superior laryngeal nerve was 67.7% (44/65) , and more than that of recurrent laryngeal nerve (18/65, 27.7%). The pyriform sinus medial wall carcinoma was 14 cases, lateral wall carcinoma 18 cases, medial and lateral wall carcinoma 36 cases. The invaded pyriform sinus apex was 34 cases, normal its apex was 26 ones, submucous invasion of its apex was 8 ones. Light lymphocytic invasion was 66.2% (45/68) and seen most in pyriform sinus carcinoma. Submucous and leaping invasion of pyriform sinus carcinoma were 24 and 8 cases. CONCLUSIONS: Intralaryngeal invasion of pyriform sinus carcinoma arose through paraglottic space first. Laryngeal cartilage membrane and their cartilage were anatomical obstacle against cancerous invasion. Lateral cricoarytenoid muscle, posterior cricoarytenoid one, thyroarytenoid and interarytenoid ones were often invaded. Pyriform sinus medial wall carcinoma invaded intralaryngeal structure easily, its lateral wall carcinoma may invade upward, downward and outward along thyroid cartilage interior wall, medial and lateral wall carcinoma may invade intralaryngeal and extralaryngeal structure, and was the most serious lesion. The invasion of pyriform sinus apex is an important sign of pyriform sinus carcinoma spreading downward to inferior and peripheral tissues of hypopharynx.

Adult↗
PubMed2005

Intranasal CpG-oligodeoxynucleotide is a potent adjuvant of vaccine against Helicobacter pylori, and T helper 1 type response and interferon-gamma correlate with the protection.

BACKGROUND: Although a series of vaccines against Helicobacter pylori have emerged in the past 10 years, the mechanism involved in their protective effect is yet to be elucidated, and more effective vaccine adjuvants remain to be developed. In this study, CpG-oligodeoxynucleotide (CpG-ODN) was investigated as a new candidate for a H. pylori vaccine adjuvant. Furthermore, the role of T helper 1 (Th1) type response and interferon (IFN)-gamma in the protective immunity was explored. METHODS: C57BL/6 mice and IFN-gamma knockout mice were intranasally or orally immunized with H. pylori whole cell sonicate (WCS)/CpG-ODN and challenged with different doses [5 x 10(8) and 5 x 10(6) colony-forming units (CFU)] of H. pylori. The protective effect was assessed as the percentage of noninfected mice. The responsive antibodies and cytokines were analyzed using an enzyme-linked immunosorbent assay (ELISA) and flow cytometry. RESULTS: The prevention rates against H. pylori infection in mice intranasally immunized with WCS plus CpG-ODN were dramatically higher than those in sham-immunized mice (70% vs. 0%, challenged with 5 x 10(8) CFU H. pylori; 90% vs. 20%, challenged with 5 x 10(6) CFU H. pylori). Significantly higher levels of immunoglobulin G2a (IgG2a) and IFN-gamma were detected in the mice immunized with WCS/CpG than in sham-immunized controls. However, vaccination failed to effectively protect IFN-gamma knockout mice challenged with H. pylori. CONCLUSIONS: CpG-ODN given intranasally is a potent adjuvant for development of a H. pylori vaccine. Th1-type response and IFN-gamma are involved in the protection.

Adjuvants, Immunologic↗
PubMed2005

[Safety of Helicobacter pylori 'test-and-treat' strategies for the management of uninvestigated dyspepsia].

OBJECTIVE: To verify the safety of Helicobacter pylori (Hp) 'test-and-treat' and 'test-and-endoscopy' strategies for management of uninvestigated dyspepsia in Shanghai patients. METHODS: 14,101 consecutive patients presented with dyspepsia receiving endoscopy during Oct. 2002 to Dec. 2003 were retrospectively analyzed in this study. The detective rate of esophageal or gastroduodenal malignancies and alarm symptoms were investigated and Hp status was assessed. RESULTS: Total 202 (1.43%) malignancies were found, including gastric cancer in 162 (1.15%), malignant gastric lymphoma in 4, esophageal cancer in 35 (0.25%) and duodenal cancer in 1. Among those patients with malignancies, ninety-nine (49.0%) patients were infected with Hp and 108 (53.5%) presented with alarm symptoms. Eighteen patients (0.46%) under the age of 45 years were diagnosed as gastric cancer. Among these patients, 5 (27.8%) presented with alarm symptoms and 13 (72.2%) were infected with Hp. If the Hp 'test-and-treat'strategy was used in dyspeptic patients under the age of 45 years without alarm symptoms in Shanghai region, then 13 cases (72.2%) of gastric cancer would be missed. If the Hp 'test-and-endoscopy' strategy was applied, then 3 cases (16.7%) of gastric cancer would be missed. CONCLUSIONS: Hp 'test-and-treat' and 'test-and-endoscopy' strategies are all not suitable for the management of uninvestigated dyspepsia in Shanghai patients. For most Shanghai adult dyspeptic patients, prompt endoscopy should be recommended as the first-line initial management option.

Adolescent↗
PubMed2005

[A skin cell segregating control system based on PC].

A skin cell segregating control system based on PC (personal computer) is presented in this paper. Its front controller is a single-chip microcomputer which enables the manipulation for 6 patients simultaneously, and thus provides a great convenience for clinical treatments for vitiligo. With the use of serial port communication technology, it's possible to monitor and control the front controller in a PC terminal. And the application of computer image acquisition technology realizes the synchronous acquisition of pathologic shin cell images pre/after the operation and a case history. Clinical tests prove its conformity with national standards and the pre-set technological requirements.

Cell Separation↗
PubMed2003

[The role of Th1-type response and gamma-interferon in the protection induced by Helicobacter pylori vaccine].

OBJECTIVE: To determine whether CpG-oligodeoxynucleotide (CpG-ODN) can be an adjuvant for the development of a Helicobacter pylori (H. pylori) vaccine, and explore the role of Th1-type response and gamma-interferon (gamma-IFN) in protection induced by vaccine. METHODS: Mice were intranasally or orally immunized with H. pylori whole cell sonicate (WCS) and CpG-ODN and challenged with 5 x 10(6) or 5 x 10(8) organisms. The mice were killed 2 and 8 weeks later respectively, the sera, saliva, gastric juice were collected to measure the concentrations of IgG, IgG1, IgG2a and IgA by ELISA, their spleen cells were isolated for analysis of intracellular gamma-IFN and interleukin-4 (IL-4) by flow cytometry. RESULTS: The prevention rate against H. pylori infection was 70% in mice intranasally immunized with WCS plus CpG-ODN and challenged with 5 x 10(8) CFU H. pylori, and 90% in those challenged with 5 x 10(6) CFU H. pylori. Significantly higher levels of IgG1 and IFN-gamma were found in the mice immunized with WCS/CpG than those in sham-immunized controls (P < 0.05). Spontaneous elimination of H. pylori infection occurred in two of ten sham-immunized mice, whose IgG1 and IFN-gamma levels were higher than those of other sham-immunized ones. CONCLUSION: CpG-Oligodeoxynucleotide is a potent adjuvant of vaccine against H. pylori, and Th1-type response and gamma-IFN are highly associated with the protective effect.

Animals↗
PubMed2003

[The role of nuclear factor kappa B in secretion of interleukin-8 by gastric cancer cell line SGC 7901 induced by Helicobacter pyiori].

OBJECTIVE: To investigate the role of nuclear factor kappaB (NF-kappaB) in the gastric inflammation induced by Helicobacter pylori (H. pylori). METHODS: SGC-7901 was transfected with IkappaB (NF-kappaB inhibitor gene) by electroporation, (beta)lacZ activity assay was used to examine transfected efficacy. Expression of IkappaB was assessed by Western-blot. Different concentration of live and heat-killed Hp (ATCC 43504) and supernatant of liquid culture were cocultured with SGC7901-IkappaB and its negative control SGC7901- neo. Activation of intracellular NF-kappaB was examined by electrophoretic mobility shift analyses (EMSA) and luciferase report gene assay at different time point. IL-8 levels were measured by ELISA at different time point. RESULTS: IL-8 release was evident 4 hours after infection of SGC-7901-neo with H. pylori, and this effect was dose-time dependent. SGC-7901-IkappaB in which NF-kappaB has not been activated could not secret IL-8 after infection with H. pylori. CONCLUSION: Secretion of IL-8 by gastric epithelial cell upon H pylori infection is dependent on activation of NF-kappaB.

Cell Line, Tumor↗