Wenhui Zhong | PubMed Health

PubMed2004

[Methods for studying soil microbial diversity].

This paper gave a review on the main methods for studying soil microbial diversity. Traditionally, the analysis of soil microbial communities relied on culturing techniques, using a variety of culture media. However, only a small fraction of the soil microbial community has been cultured and isolated with this approach. Other methods such as Biolog GN analysis, phospholipids fatty acids analysis and nucleic acid-based analysis can be used to study and characterize soil microbes which currently cannot be cultured, and to get more and complete information about soil microbial community.

Biodiversity↗

PubMedDate not supplied

Thermochemical characteristics of 2,4-dichlorophenol degrading strain Pseudomonas GT241-1 of growth metabolism by microcalorimetry.

By using LKB-2277 Bioactivity Monitor, ampoule method, the heat output of the growth metabolism of a 2.4-dichlorophenol degrading bacteria strain, Pseudomonas strain GT241-1, has been determined at 30 degrees C. From the thermogenic curves, it can be established that thermokinetic equation of their growth metabolism is Pt = Pt = 0 exp(k(m) t), dP/dt = k(m)P1, with the order of growth metabolism n = 1. The experimental results indicate that the relationship between the metabolic power (P) and the cell concentration (C), and relationship between the metabolic power of each cell (P0) and the cell concentration can be characterized by the following thermal equation: C = a + kP, InC = a' + k'P0 or dC/dP0 = KC1. The order of the P0-C equation n is also 1. These results are very significant in environmental sciences, biology and thermochemistry.

Biodegradation, Environmental↗