Biomedical subjects

Wenji Wang

Publications and source records attributed to Wenji Wang.

6 recordsLinked to original sources

PubMed2025

Integration of multiple omics reveals key targets and cellular mechanisms for intervention in sarcopenia.

BACKGROUND: Sarcopenia, an age-related syndrome characterized by progressive loss of muscle mass, strength, and function, presents a significant global health burden with limited therapeutic interventions. This study integrates genomic causality, multi-tissue omics, and cellular mediation analyses to identify and prioritize mechanistically grounded therapeutic targets. METHODS: A multi-tiered analytical framework was applied, beginning with two-sample Mendelian randomization (MR) to infer causal relationships between 4907 plasma proteins (cis-pQTLs from 35,559 individuals) and sarcopenia traits in Pan-UK Biobank participants. Bayesian colocalization and transcriptomic validation in human sarcopenia muscle biopsies were employed to prioritize targets. Cellular mediation analysis quantified contributions of immune and stromal cell subtypes to protein-trait pathways using transcriptomic deconvolution. RESULTS: MR identified 1237 plasma proteins causally associated with sarcopenia traits, with six targets (HGFAC, GATM, HMOX2, F2, LMAN2L, HPGDS) validated through colocalization, transcriptomic expression, and sarcopenia-related dysregulation. Cellular mediation revealed immune mechanisms underlying HGFAC's effects, with CD4+ regulatory T cells mediating 3.49 % of its impact on sarcopenia traits. Prothrombin exhibited muscle-protective effects independent of coagulation. CONCLUSION: This study establishes a causal map linking plasma proteins to sarcopenia through immune-stromal interactions. The integration of MR, multi-omics validation, and cellular mediation prioritizes six proteins as actionable targets, supporting repurposing of thrombin inhibitors and development of immunometabolic therapies. The framework bridges genomic causality with cellular pathophysiology, advancing precision strategies for age-related muscle decline.

Humans↗

PubMed2006

Expression of survivin and correlation with PCNA in osteosarcoma.

BACKGROUND AND OBJECTIVES: Survivin is a new anti-apoptosis gene with reported expression during fetal development and in cancer tissues. Proliferating cell nuclear antigen (PCNA) is essential for the replication of deoxyribonucleic acid (DNA). In this experiment, we examined the expression of survivin and PCNA in human osteosarcoma tissues and investigating whether survivin and PCNA expressions were correlated with histological grades, histological types, and patients' clinical characteristics. METHODS: Sixty-eight osteosarcoma samples were divided into two groups based on the degree of tumor cell differentiation. Immunohistochemical staining was performed to analyze the expression of survinin and PCNA. Four histological types and patients' clinical characteristics were also recorded. RESULTS: There were abundant levels of survivin and PCNA immunoreactivity in the nucleus and/or cytoplasm of the osteosarcoma cells. All cells essentially revealed the cytoplasmic localization and the nuclear signals of survivin in same cases, while PCNA from the majority of cases predominantly showed nuclear expression. Scoring on the percentage of positive cells indicated that survivin expression was significantly associated with the PCNA-labeling index (LI), which was correlated with the histological grades of osteosarcoma (chi2 = 17.86 and chi2 = 23.75, respectively). CONCLUSIONS: The expression of survivin can be used as a useful indicator in grading the malignancy of osteosarcoma.

Adolescent↗

PubMed2006

Dendritic abnormalities in retinal ganglion cells of three-month diabetic rats.

PURPOSE: To determine the effect of three-month diabetes on the retinal ganglion cell (RGC) morphology and density. METHODS: Experimental diabetes was induced in Sprague-Dawley rats by intraperitoneal injection of streptozotocin (STZ). Retinas from three-month diabetic and age-matched control rats were harvested, and immunohistochemistry with monoclonal anti-Thy-1 antibody was carried out for calculating RGC density. Random RGC labeling with gene gun propelled lipophilic fluorescent dye, DiI, coated particles (DiOlistic method) was done for detailed RGC classification, dendritic field, and soma size measurement. RESULTS: The number of Thy1-labeled RGCs in the three-month diabetic rats was significantly reduced compared with that in the age-matched control. Obvious RGC morphology changes were observed, and the number of RGCs that could not be classified was significantly greater in the diabetic retinas. Among those well-classified RGCs, cells with enlarged dendritic fields were more frequently seen in the RGA group (401+/-86 um, n = 59, P < 0.001), but the soma sizes were unchanged from the controls (P > 0.05). For cells in the groups of RGB and RGC, no significant changes in the dendritic fields and soma sizes were found (P > 0.05). CONCLUSIONS: In the three-month STZ-induced diabetic rat, retinal ganglion cell loss is associated with morphology change. The surviving RGCs in the diabetic retina, especially those in RGA group, show significant dendritic field enlargement. This plasticity of the surviving RGC dendrites may represent a compensatory response to the overall loss of RGCs in diabetes.

Animals↗

PubMed2006

Retinoschisis at macular area in highly myopic eye by optic coherence tomography.

PURPOSE: To improve our understanding of macular schisis in highly myopic eyes. METHODS: From 2002.6-2003.12, the highly myopic eyes (> 6D) which has macular schisis found by OCT at our hospital were included in the study. Each eye then received further examinations including visual acuity, refractive error and ultrasonography. Then the results were analyzed to find the characteristic of the entity. RESULTS: During that period, OCT found macular schisis in 38 highly myopic eyes from 36 patients. Their vision was low, and their average axial length and refractive error were 29.6mm and -10.5D respectively. The appearance and the area of the schisis are different among the eyes. But the schisis can be largely divided into two types: inner and outer schisis. They can exit independently or concurrently in the same eye. And the schisis was often accompanied by other macular findings, like retinal detachment, macular hole. CONCLUSION: Macular schisis in highly myopic eyes is not uncommon in the clinic. OCT is useful in its diagnosis.

Female↗

PubMed2002

[Vitrectomy for severe Coats' disease].

OBJECTIVE: To investigate the outcome of vitreoretinal surgery for severe Coats' disease. METHODS: From December 1995 to December 1999, trans pars plana vitrectomy was performed in 11 patients (11 eyes) diagnosed as Coats' disease. Ten of them were complicated with total retinal detachment, and the other one had tractional retinal detachment caused by epiretinal proliferation. All these patients were followed up from 3 months to 4.5 years. RESULTS: All of the patients obtained useful vision except 1 patient whose operation was given up because of severe shortening of the retina. Three patients had the visual acuity higher than 0.02, and the best vision was 0.2. Obliteration of all abnormal vessels and resorption of all exudate was found postoperatively from 1 month to 3 months. Although the retina had been reattached in 10 patients during the operation, re-detachment was found in 4 patients postoperatively. After therapy all re-detached retina was reattached. The success rate was 90.9%. CONCLUSIONS: Vitrectomy with endolaser or scleral cryotherapy is an ideal therapy for severe Coats' disease, although the visual prognosis is still limited. It can preserve the limited vision, and prevent the severe complications such as rubeosis iridis, glaucoma, and phthisis bulbi.

Adolescent↗

PubMed2002

High-resolution imaging of living retina through optic adaptive retinal imaging system.

PURPOSE: To evaluate the possibility as well as the usage of adaptive optics in high-resolution retinal imaging. METHODS: From March to November 2001, the fundus of 25 adults were checked by using Optic Adaptive Retinal Imaging System (OAS). The age of the subjects varied from 18 - 48 years. All had normal visual acuity from 0.9 to 1.0. No abnormality was found in the ocular examination, and their medical as well as ocular history was unremarkable. RESULTS: High-resolution images of the retinal cells, photoreceptor and bipolar cell, were analysed. In these images, the cells are clearly resolved. The density of the photoreceptor at area 1.5 degree from the foveloa is around 40 000 - 50 000/mm2. At area 3 degree, it drops to less than 30 000/mm2. CONCLUSION: Optic Adaptive Retinal Imaging System (AOS) is able to get high-resolution image of retinal cells in living human eyes. It may be widely used in ophthalmology experimentally and clinically.

Adolescent↗