Biomedical subjects
Publications and source records attributed to Wenwei Zhong.
We demonstrate experimentally the three-dimensional reconstructions of fluorescent biological specimens using scanning holographic microscopy. Three-dimensional reconstructions with transverse resolution below about 1 microm of transmission and fluorescence emission images are presented and analyzed. The limitations of the method are discussed.
Scanning holographic microscopy is a two-pupil synthesis method allowing the capture of single-sideband in-line holograms of noncoherent (e.g., fluorescent) three-dimensional specimens in a single two-dimensional scan. The flexibility offered by the two-pupil method in synthesizing unusual point-spread functions is discussed. We illustrate and compare three examples of holographic recording, using computer simulations. The first example is the classical hologram in which each object point is encoded as a spherical wave. The second example uses pupils with spherical phase distributions having opposite curvatures, leading to reconstructed images with a resolution limit that is half that of the objective. In the third example, axicon pupils are used to obtain axially sectioned images.
OBJECTIVE: To explore the clinical features and the treatment result of occlusal surface bone tumors. METHOD: Sixty-seven patients with occlusal surface bone tumors were retrospective analyzed. RESULT: Benign and malignant tumors were respectively 57 (85%) and 10 (15%). Significant differences (P < 0.05) were observed between the age of male and female. Fifty-five patients followed up from 8 months to 10 years, fifteen patients closed to follow up. Five cases were found local recurrence of tumors in 52 patients. Fifteen patients were performed partial resection of mandible, among of them, 12 mandible defects were reconstructed simultaneously and the reconstruction, survived well without failure. CONCLUSION: Polycystic disease of the jaw are usually handled by curettage; Auto-bone transplantation is still a good method in the reconstruction of mandible defects.