A critique of oocyte-assisted reprogramming.
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Catholicism↗
Biomedical subjects
William Burke
Publications and source records attributed to William Burke.
Preliminary experience with robot-assisted laparoscopic myomectomy.
The following retrospective case series evaluated the technique and feasibility of integrating robot-assisted technology in the performance of a laparoscopic myomectomy in order to overcome the limitations of conventional laparoscopy. We attempted 35 robot-assisted laparoscopic myomectomies in a university hospital setting with a conversion rate of 8.6%. There were a total of 48 myomas removed in 31 patients with completed robot-assisted laparoscopy. The mean number of myomas removed/patient was 1.6 (range 1-5). The mean diameter of myomas removed was 7.9 +/- 3.5 cm (95% CI 6.63-9.13), with the majority greater than 5 cm. The mean myoma weight was 223.2 +/- 244.1 g (95% CI 135.8-310.6). Mean operating time was 230.8 +/- 83 minutes (95% CI 201.6-260). The average estimated blood loss was 169 +/- 198.7 mL (95% CI 99.1-238.4). One patient experienced cardiogenic shock from vasopressin, two developed postoperative infections, and one was found to have adenomatous adenomyosis instead of a leiomyoma. The median length of hospital stay was 1 day. Overall, robot-assisted laparoscopic myomectomy is a promising new technique that may overcome many of the surgical limitations of conventional laparoscopy.
Adult↗
Motion sensitivity in cat's superior colliculus: contribution of different visual processing channels to response properties of collicular neurons.
It is well established that neurons in the retinorecipient layers of superior colliculus (SC), the mammalian homologue of the optic tectum of other vertebrates, are extremely sensitive to moving stimuli. In our studies we have distinguished several functionally distinct groups of neurons in the retinorecipient layers of the SC of the cat on the basis of their velocity response profiles. Our data revealed substantial convergence of the Y and non-Y information channels on single SC neurons. Second, using the method of selective conduction block of the Y-type fibers in one optic nerve we have shown that responses of SC cells to high-velocity motion are dependant on the integrity of Y-type input. Third, in order to determine the degree of influence of the X- and W-type input on cellular responses we have examined spatial and temporal frequency response profiles of single collicular neurons using sinusoidal gratings drifting in the preferred direction. At any given eccentricity, most collicular neurons exhibited a preference for relatively very low spatial frequencies. The preference for low spatial frequencies combined with temporal frequency profiles of collicular neurons suggests that the Y- and W-type inputs constitute the major functional inputs to the retinorecipient layers of the SC and that the "top-down" X-type input from the visual cortex has only a minor impact on the spatio-temporal frequency response profiles of collicular receptive fields.
Animals↗
Modulation of signal transducer and activator of transcription 3 activities by p53 tumor suppressor in breast cancer cells.
The constitutive activation of the Stat3 oncogene product and mutation of the p53 tumor suppressor are both frequently detected in human breast cancer. We sought to determine whether there is functional regulation of Stat3 by wild-type (wt) p53. We demonstrate that expression of wt p53, but not mutant p53, significantly diminished phosphorylation of Stat3, reduced Stat3 DNA binding activity, and inhibited Stat3-dependent transcriptional activity in breast cancer cells expressing constitutively active Stat3. Expression of wt p53 did not cause a reduction in the phosphorylation of three unrelated protein kinases in other signal transduction pathways, AKT, extracellular signal-regulated kinase (ERK)1, and ERK2 or a reduction of phosphorylation of epidermal growth factor receptor. Furthermore, the expression of the p53 downstream target, p21(WAF-1), did not have an inhibitory effect on Stat3 phosphorylation. Wt p53 also induced significant apoptosis in breast cancer cell lines that express constitutively active Stat3. Interestingly, the p53-dependent apoptosis occurred in the presence of high levels of phosphorylated AKT and ERK1/2. Therefore, these findings demonstrate a novel p53-dependent cellular process that regulates Stat3 phosphorylation and activity.
Apoptosis↗