Biomedical subjects

William D O'Brien

Publications and source records attributed to William D O'Brien.

At least 19 recordsLinked to original sources

PubMed2006

Ultrasound-biophysics mechanisms.

Ultrasonic biophysics is the study of mechanisms responsible for how ultrasound and biological materials interact. Ultrasound-induced bioeffect or risk studies focus on issues related to the effects of ultrasound on biological materials. On the other hand, when biological materials affect the ultrasonic wave, this can be viewed as the basis for diagnostic ultrasound. Thus, an understanding of the interaction of ultrasound with tissue provides the scientific basis for image production and risk assessment. Relative to the bioeffect or risk studies, that is, the biophysical mechanisms by which ultrasound affects biological materials, ultrasound-induced bioeffects are generally separated into thermal and non-thermal mechanisms. Ultrasonic dosimetry is concerned with the quantitative determination of ultrasonic energy interaction with biological materials. Whenever ultrasonic energy is propagated into an attenuating material such as tissue, the amplitude of the wave decreases with distance. This attenuation is due to either absorption or scattering. Absorption is a mechanism that represents that portion of ultrasonic wave that is converted into heat, and scattering can be thought of as that portion of the wave, which changes direction. Because the medium can absorb energy to produce heat, a temperature rise may occur as long as the rate of heat production is greater than the rate of heat removal. Current interest with thermally mediated ultrasound-induced bioeffects has focused on the thermal isoeffect concept. The non-thermal mechanism that has received the most attention is acoustically generated cavitation wherein ultrasonic energy by cavitation bubbles is concentrated. Acoustic cavitation, in a broad sense, refers to ultrasonically induced bubble activity occurring in a biological material that contains pre-existing gaseous inclusions. Cavitation-related mechanisms include radiation force, microstreaming, shock waves, free radicals, microjets and strain. It is more challenging to deduce the causes of mechanical effects in tissues that do not contain gas bodies. These ultrasonic biophysics mechanisms will be discussed in the context of diagnostic ultrasound exposure risk concerns.

Biophysical Phenomena↗

PubMed2006

Vascular lesions and s-thrombomodulin concentrations from auricular arteries of rabbits infused with microbubble contrast agent and exposed to pulsed ultrasound.

Arterial injury resulting from the interaction of contrast agent (CA) with ultrasound (US) was studied in rabbit auricular arteries and assessed by histopathologic evaluation and s-thrombomodulin concentrations. Three sites on each artery were exposed (2.8 MHz, 5-min exposure duration, 10-Hz pulse repetition frequency, 1.4-mus pulse duration) using one of three in situ peak rarefactional pressures (0.85, 3.9 or 9.5 MPa). Saline, saline/CA, and saline/US infusion groups (n = 28) did not have histopathologic damage. The saline/CA/US infusion group (n = 10) at exposure conditions below the FDA mechanical index limit of 1.9 did not have histopathologic damage, whereas the saline/CA/US infusion group (n = 9) at exposure conditions above the FDA limit did have damage (5 of 9 arteries). Lesions were characteristic of acute coagulative necrosis. Mean s-thrombomodulin concentrations, a marker for endothelial cell injury, were highest in rabbits exposed to US at 0.85 and 3.9 MPa, suggesting that vascular injury may be physiological and not accompanied by irreversible cellular injury.

Albumins↗

PubMed2006

Threshold estimation of ultrasound-induced lung hemorrhage in adult rabbits and comparison of thresholds in mice, rats, rabbits and pigs.

The objective of this study was to assess the threshold and superthreshold behavior of ultrasound (US)-induced lung hemorrhage in adult rabbits to gain greater understanding about species dependency. A total of 99 76 +/- 7.6-d-old 2.4 +/- 0.14-kg New Zealand White rabbits were used. Exposure conditions were 5.6-MHz, 10-s exposure duration, 1-kHz PRF and 1.1-micros pulse duration. The in situ (at the pleural surface) peak rarefactional pressure, p(r(in situ)), ranged between 1.5 and 8.4 MPa, with nine acoustic US exposure groups plus a sham exposure group. Rabbits were assigned randomly to the 10 groups, each with 10 rabbits, except for one group that had nine rabbits. Rabbits were exposed bilaterally with the order of exposure (left then right lung, or right then left lung) and acoustic pressure both randomized. Individuals involved in animal handling, exposure and lesion scoring were blinded to the exposure condition. Probit regression analysis was used to examine the dependence of the lesion occurrence on in situ peak rarefactional pressure and order of exposure (first vs. second). Likewise, lesion depth and lesion root surface area were analyzed using Gaussian tobit regression analysis. Neither probability of a lesion nor lesion size measurements was found to be statistically dependent on the order of exposure after the effect of p(r(in situ)) was considered. Also, a significant correlation was not detected between the two exposed lung sides on the same rabbit in either lesion occurrence or size measures. The p(r(in situ)) threshold estimates (in MPa) were similar to each other across occurrence (3.54 +/- 0.78), depth (3.36 +/- 0.73) and surface area (3.43 +/- 0.77) of lesions. Using the same experimental techniques and statistical approach, great consistency of thresholds was demonstrated across three species (mouse, rat and rabbit). Further, there were no differences in the biologic mechanism of injury induced by US and US-induced lesions were similar in morphology in all species and age groups studied. The extent of US-induced lung damage and the ability of the lung to heal led to the conclusion that, although US can produce lung damage at clinical levels, the degree of damage does not appear to be a significant medical problem.

Animals↗

PubMed2006

Lesions of ultrasound-induced lung hemorrhage are not consistent with thermal injury.

Thermal injury, a potential mechanism of ultrasound-induced lung hemorrhage, was studied by comparing lesions induced by an infrared laser (a tissue-heating source) with those induced by pulsed ultrasound. A 600-mW continuous-wave CO2 laser (wavelength approximately 10.6 microm) was focused (680-microm beamwidth) on the surface of the lungs of rats for a duration between 10 to 40 s; ultrasound beamwidths were between 310 and 930 microm. After exposure, lungs were examined grossly and then processed for microscopic evaluation. Grossly, lesions induced by laser were somewhat similar to those induced by ultrasound; however, microscopically, they were dissimilar. Grossly, lesions were oval, red to dark red and extended into subjacent tissue to form a cone. The surface was elevated, but the center of the laser-induced lesions was often depressed. Microscopically, the laser-induced injury consisted of coagulation of tissue, cells and fluids, whereas injury induced by ultrasound consisted solely of alveolar hemorrhage. These results suggest that ultrasound-induced lung injury is most likely not caused by a thermal mechanism.

Animals↗

PubMed2006

A regularized inverse approach to ultrasonic pulse-echo imaging.

An inverse-theoretic approach to ultrasonic pulse-echo imaging based on nonquadratic regularization is presented, and its effectiveness is investigated computationally by: 1) evaluating the quality of the reconstruction of speckle-based images as a function of the transmit-receive bandwidth and focal number of the transducer; 2) comparing the reconstructed images with those obtained by using conventional B-mode imaging. The L-curve and the generalized cross-validation methods were evaluated as automatic regularization parameter selection techniques. The inversion using regularization produced better results than conventional B-mode imaging for high signal-to-noise ratios (SNRs). A lower bound of 30 dB for the SNR was found for this study, below which several of the image features were lost during the reconstruction process in order to control the distortion due to the noise.

Algorithms↗

PubMed2006

Ultrasonic contrast agent shell rupture detected by inertial cavitation and rebound signals.

Determining the rupture pressure threshold of ultrasound contrast agent microbubbles has significant applications for contrast imaging, development of therapeutic agents, and evaluation of potential bioeffects. Using a passive cavitation detector, this work evaluates rupture based on acoustic emissions from single, encapsulated, gas-filled microbubbles. Sinusoidal ultrasound pulses were transmitted into weak solutions of Optison at different center frequencies (0.9, 2.8, and 4.6 MHz), pulse durations (three, five, and seven cycles of the center frequencies), and peak rarefactional pressures (0.07 to 5.39 MPa). Pulse repetition frequency was 10 Hz. Signals detected with a 13-MHz, center-frequency transducer revealed postexcitation acoustic emissions (between 1 and 5 micros after excitation) with broadband spectral content. The observed acoustic emissions were consistent with the acoustic signature that would be anticipated from inertial collapse followed by "rebounds" when a microbubble ruptures and thus generates daughter/free bubbles that grow and collapse. The peak rarefactional pressure threshold for detection of these emissions increased with frequency (e.g., 0.53, 0.87, and 0.99 MPa for 0.9, 2.8, and 4.6 MHz, respectively; five-cycle pulse duration) and decreased with pulse duration. The emissions identified in this work were separated from the excitation in time and spectral content, and provide a novel determination of microbubble shell rupture.

Albumins↗

PubMed2006

Impact of local attenuation approximations when estimating correlation length from backscattered ultrasound echoes.

Estimating the characteristic correlation length of tissue microstructure from the backscattered power spectrum could improve the diagnostic capability of medical ultrasound. Previously, size estimates were obtained after compensating for source focusing, the frequency-dependent attenuation along the propagation path (total attenuation), and the frequency-dependent attenuation in the scattering region (local attenuation). In this study, the impact of approximations of the local attenuation on the scatterer size estimate was determined using computer simulations and theoretical analysis. The simulations used Gaussian impedance distributions with an effective radius of 25 microm randomly positioned in a homogeneous half-space sonified by a spherically focused source (f/1 to f/4). The approximations of the local attenuation that were assessed neglected local attenuation (i.e., assume 0 dB/cm-MHz) neglected frequency dependence of the local attenuation, and assumed a finite frequency dependence (i.e., 0.5 dB/cm-MHz) independent of the true attenuation of the medium. Errors in the scatterer size estimate due to the local attenuation approximations increased with increasing window length, increasing true local attenuation and increasing f number. The most robust estimates were obtained when the local attenuation was approximated by a tissue-independent attenuation value that was greater than 70% of the largest attenuation expected in the tissue region of interest.

Algorithms↗

PubMed2006

Application of three scattering models to characterization of solid tumors in mice.

Two mouse models of mammary cancer (a carcinoma and sarcoma) were examined using quantitative ultrasound (QUS) and three models for ultrasound backscattering. The first model that was examined was the spherical Gaussian model (SGM). The second model was the fluid-filled sphere model (FFSM) and was hypothesized to model scattering from cell nuclei with the cell cytoplasm acting as background. The third model, called the new cell model (NCM), was constructed to model backscattering from cell nuclei and cytoskeleton. The average scatterer diameters (ASDs) were estimated from regions-of-interest (ROIs) inside the tumors using all three models and then compared. The ultrasound analysis bandwidth used in the study was 16 to 27 MHz. QUS images of the tumors utilizing the ASD estimates from the three models were constructed. The ASDs were 30.3 +/- 3.06 microm and 25.2 +/- 4.01 microm with the SGM, 47.3 +/- 7.99 microm and 47.7 +/- 7.01 microm with the FFSM and 41.2 +/- 1.39 microm and 34.4 +/- 5.95 microm with the NCM for the carcinoma and sarcoma, respectively, statistically significant differences between the ASD estimates from the carcinomas and sarcomas were observed using the SGM and NCM but not with the FFSM.

Acoustics↗

PubMed2006

Superthreshold behavior of ultrasound-induced lung hemorrhage in adult rats: role of pulse repetition frequency and pulse duration.

OBJECTIVE: The purpose of this study was to enhance the findings of an earlier ultrasound-induced lung hemorrhage study (Ultrasound Med Biol 2003; 29:1625-1634) that estimated pressure thresholds as a function of pulse duration (PD: 1.3, 4.4, 8.2, and 11.6 micros; 2.8 MHz; 10-s exposure duration [ED]; 1-kHz pulse repetition frequency [PRF]). In this study, the roles of PRF and PD were evaluated at 5.9 MPa, the peak rarefactional pressure threshold near that of the ED50 estimate previously determined. METHODS: A 4 x 4 factorial design study (PRF: 50, 170, 500, and 1700 Hz; PD: 1.3, 4.4, 8.2, and 11.6 mus) was conducted (2.8 MHz; 10-s ED). Sprague Dawley rats (n = 175) were divided into 16 exposure groups (10 rats per group) and 1 sham group (15 rats); no lesions were produced in the sham group. Logistic regression analysis evaluated significance of effects for lesion occurrence, and Gaussian tobit analysis evaluated significance for lesion depth and surface area. RESULTS: For lesion occurrence and sizes, the main effect of PRF was not significant. The interaction term, PRF x PD, was highly significant, indicating a strong positive dependence of lesion occurrence on the duty factor. The main effect of PD was almost significant (P = .052) and thus was included in the analysis model for a better fit. CONCLUSIONS: Compared with the findings from a PRF x ED factorial study (J Ultrasound Med 2005; 24:339-348), a function that considers PRF, PD, and ED might yield a sensitive indicator for consideration of a modified mechanical index, at least for the lung.

Age Factors↗

PubMed2006

Quantitative ultrasound assessment of the rat cervix.

OBJECTIVE: The purpose of this research was to detect cervical ripening with a new quantitative ultrasound technique. METHODS: Cervices of 13 nonpregnant and 65 timed pregnant (days 15, 17, 19, 20, and 21 of pregnancy) Sprague Dawley rats were scanned ex vivo with a 70-MHz ultrasound transducer. Ultrasound scatterer property estimates (scatterer diameter [SD], acoustic concentration [AC], and scatterer strength factor [SSF]) from the cervices were quantified and then compared to hydroxyproline and water content. Insertion loss (attenuation) was measured in 3 rats in each of the 6 groups. Discriminant analysis was used to predict gestational age group (cervical ripening) from the ultrasound variables SD, SSF, and AC. RESULTS: Differences were observed between the groups (SD, AC, and SSF; P < .0001). Quantitative ultrasound measures changed as the cervix ripened: (1) SD increased from days 15 to 21; (2) AC decreased from days 15 to 21; and (3) SSF was the greatest in the nonpregnant group and the least in the day 21 group. Cervix hydroxyproline content increased as pregnancy progressed (P < .003) and correlated with group, SD, AC, and SSF (P < .001). Discriminant analysis of ultrasound variables predicted 56.4% of gestational group assignment (P < .001) and increased to 77% within 2 days of the predicted analysis. Cervix insertion loss was greatest for the nonpregnant group and least for the day 21 group. CONCLUSIONS: Quantitative ultrasound predicted cervical ripening in the rat cervix, but before use in humans, quantitative ultrasound will need to predict gestational age in the later days of gestation with more precision.

Analysis of Variance↗

PubMed2005

Evaluation of the spectral fit algorithm as functions of frequency range and deltakaeff.

Considerable effort has been directed at quantifying the properties of the tissue microstructure (i.e., scatterer correlation length) to diagnose disease and monitor treatment. In vivo assessments have had limited success due to frequency-dependent attenuation along the propagation path (i.e., total attenuation) masking the frequency dependence of the scattering from the tissue microstructure. Previously, both total attenuation and scatterer correlation length, given by the effective radius, were solved simultaneously by a two-parameter minimization of the mean squared error between a reference spectrum, modified by the attenuation and scatterer effective radius, and the backscattered waveforms using an algorithm termed the spectral fit algorithm. Herein, the impact of frequency range (largest frequency minus smallest frequency) and deltakaeff (largest kaeff value minus smallest kaeff value; k is wave number and aeff is scatterer effective radius) used by the spectral fit algorithm on estimating the scatterer effective radius, and total attenuation was assessed by computer simulations while excluding frequencies of the backscattered power spectrum dominated by electronic noise. The simulations varied the effective radius of the scatterers (5 microm to 150 microm), the attenuation of the region (0 to 1 dB/cm-MHz), the bandwidth of the source, and the amount of electronic noise added to the radio frequency (rf) waveforms. The center frequency of the source was maintained at 8 MHz. Comparable accuracy and precision of the scatterer effective radius were obtained for all the simulations whenever the same deltakaeff was used to obtain the estimates. A deltakaeff of 1 gave an accuracy and precision of approximately 15% +/- 35%, and a width of 1.5 gave an accuracy and precision of approximately 5% +/- 15% consistently for all of the simulations. Similarly, the accuracy and precision of the total attenuation estimate were improved by increasing the frequency range used by the spectral fit algorithm.

Algorithms↗

PubMed2005

Identifying ultrasonic scattering sites from three-dimensional impedance maps.

Ultrasonic backscattered signals contain frequency-dependent information that is usually discarded to produce conventional B-mode images. It is hypothesized that parametrization of the quantitative ultrasound frequency-dependent information (i.e., estimating scatterer size and acoustic concentration) may be related to discrete scattering anatomic structures in tissues. Thus, an estimation technique is proposed to extract scatterer size and acoustic concentration from the power spectrum derived from a three-dimensional impedance map (3DZM) of a tissue volume. The 3DZM can be viewed as a computational phantom and is produced from a 3D histologic data set. The 3D histologic data set is constructed from tissue sections that have been appropriately stained to highlight specific tissue features. These tissue features are assigned acoustic impedance values to yield a 3DZM. From the power spectrum, scatterer size and acoustic concentration estimates were obtained by optimization. The 3DZM technique was validated by simulations that showed relative errors of less than 3% for all estimated parameters. Estimates using the 3DZM technique were obtained and compared against published ultrasonically derived estimates for two mammary tumors, a rat fibroadenoma and a 4T1 mouse mammary carcinoma. For both tumors, the relative difference between ultrasonic and 3DZM estimates was less than 10% for the average scatterer size.

Acoustics↗

PubMed2005

Estimation of total attenuation and scatterer size from backscattered ultrasound waveforms.

Quantitative ultrasound techniques using backscattered echoes have had limited success in vivo due to the frequency-dependent attenuation along the entire propagation path masking the frequency dependence of the backscatter. Herein, total attenuation and scatterer size are estimated simultaneously by an analysis of the in vivo backscattered power spectrum using two approaches. The simulations used to evaluate the two approaches used frequencies between 4 and 11 MHz with an effective scatterer radius of 25 microm. The first approach was based on approximations of the in vivo backscattered power spectrum (i.e., assumed Gaussian function), wherein attenuation and size were estimated by assuming each was a Gaussian transformation performed on Gaussian power spectra. The approach had poor accuracy due to the backscattered power spectra not being sufficiently modeled by a Gaussian function. The second approach estimated attenuation and size by fitting a modified reference spectrum to the in vivo backscattered power spectrum without any assumptions about the shape of the spectrum. The accuracy of the size estimate was better than 20% for signal-to-noise ratio >6 dB, window lengths greater than 4 mm, and attenuation between 0 and 1 dB/cm-MHz. However, the precision quickly degraded with increasing noise, increasing attenuation, and decreasing window length.

Algorithms↗

PubMed2005

A model for estimating ultrasound attenuation along the propagation path to the fetus from backscattered waveforms.

Accurate estimates of the ultrasound pressure and/or intensity incident on the developing fetus on a patient-specific basis could improve the diagnostic potential of medical ultrasound by allowing the clinician to increase the transmit power while still avoiding the potential for harmful bioeffects. Neglecting nonlinear effects, the pressure/intensity can be estimated if an accurate estimate of the attenuation along the propagation path (i.e., total attenuation) can be obtained. Herein, a method for determining the total attenuation from the backscattered power spectrum from the developing fetus is proposed. The boundaries between amnion and either the fetus' skull or soft tissue are each modeled as planar impedance boundaries at an unknown orientation with respect to the sound beam. A mathematical analysis demonstrates that the normalized returned voltage spectrum from this model is independent of the planes orientation. Hence, the total attenuation can be estimated by comparing the location of the spectral peak in the reflection from the fetus to the location of the spectral peak in a reflection obtained from a rigid plane in a water bath. The independence of the attenuation estimate and plane orientation is then demonstrated experimentally using a Plexiglas plate, a rat's skull, and a tissue-mimicking phantom.

Animals↗

PubMed2005

Signal processing strategies that improve performance and understanding of the quantitative ultrasound SPECTRAL FIT algorithm.

Quantifying the size of the tissue microstructure using the backscattered power spectrum has had limited success due to frequency-dependent attenuation along the propagation path, thus masking the frequency dependence of the scatterer size. Previously, the SPECTRAL FIT algorithm was developed to solve for total attenuation and scatterer size simultaneously [Bigelow et al., J. Acoust. Soc. Am. 117, 1431-1439 (2005)]. Herein, the outcomes from signal processing strategies on the SPECTRAL FIT algorithm are investigated. The signal processing methods can be grouped into two categories, viz., methods that improve the performance of the algorithm and methods that provide insight. The methods that improve the performance include compensating for the windowing function used to gate the time-domain signal, averaging the spectra in the normal frequency domain rather than the log domain to improve the precision of the scatterer size and attenuation estimates, improving the selection of the usable frequency range for the SPECTRAL FIT algorithm, and improving the compensation for electronic noise. The methods that provide insight demonstrate that the anomalous rapid fluctuations of the backscattered power spectrum do not affect the SPECTRAL FIT algorithm, and accurate attenuation estimates can be obtained even when the correct scatterer geometry (i.e., form factor) is not known.

Algorithms↗

PubMed2005

Superthreshold behavior of ultrasound-induced lung hemorrhage in adult rats: role of pulse repetition frequency and exposure duration revisited.

OBJECTIVE: The purpose of this study was to augment and reevaluate the ultrasound-induced lung hemorrhage findings of a previous 5 x 3 factorial design study (Ultrasound Med Biol 2001; 27:267-277) that evaluated the role of pulse repetition frequency (PRF: 25, 50, 100, 250, and 500 Hz) and exposure duration (ED; 5, 10, and 20 s) on ultrasound-induced lung hemorrhage at an in situ (at the pleural surface) peak rarefactional pressure [pr(in situ)] of 12.3 MPa; only PRF was found to be significant. However, saturation (response plateau) due to the high pr(in situ) might have skewed the results. In this follow-up 3 x 3 factorial design study, a wider range of PRFs and EDs were used at a lower pr(in situ). METHODS: Sprague Dawley rats (n=198) were divided into 18 ultrasonically exposed groups (10 rats per group) and 6 sham groups (3 per group). The 3 x 3 factorial design study (PRF: 17, 170, and 1700 Hz; ED: 5, 31.6, and 200 s) was conducted at 2 frequencies (2.8 and 5.6 MHz). The p(r(in situ)) was 6.1 MPa. Logistic regression analysis evaluated lesion occurrence, and Gaussian tobit analysis evaluated lesion depth and surface area. RESULTS: Frequency did not have a significant effect, so the analysis combined results for the 2 frequencies. For lesion occurrence and sizes, the main effects for PRF and ED were not significant. The interaction term was highly significant, indicating a strong dependence of lesion occurrence and size on the total number of pulses (PRF x ED). CONCLUSIONS: The results of both studies are consistent with the hypothesis that the total number of pulses is an important factor in the genesis of ultrasound-induced lung hemorrhage.

Age Factors↗

PubMed2005

Interlaboratory comparison of ultrasonic backscatter coefficient measurements from 2 to 9 MHz.

OBJECTIVE: As are the attenuation coefficient and sound speed, the backscatter coefficient is a fundamental ultrasonic property that has been used to characterize many tissues. Unfortunately, there is currently far less standardization for the ultrasonic backscatter measurement than for the other two, as evidenced by a previous American Institute of Ultrasound in Medicine (AIUM)-sponsored interlaboratory comparison of ultrasonic backscatter, attenuation, and speed measurements (J Ultrasound Med 1999; 18:615-631). To explore reasons for these disparities, the AIUM Endowment for Education and Research recently supported this second interlaboratory comparison, which extends the upper limit of the frequency range from 7 to 9 MHz. METHODS: Eleven laboratories were provided with standard test objects designed and manufactured at the University of Wisconsin (Madison, WI). Each laboratory was asked to perform ultrasonic measurements of sound speed, attenuation coefficients, and backscatter coefficients. Each laboratory was blinded to the values of the ultrasonic properties of the test objects at the time the measurements were performed. RESULTS: Eight of the 11 laboratories submitted results. The range of variation of absolute magnitude of backscatter coefficient measurements was about 2 orders of magnitude. If the results of 1 outlier laboratory are excluded, then the range is reduced to about 1 order of magnitude. Agreement regarding frequency dependence of backscatter was better than reported in the previous interlaboratory comparison. For example, when scatterers were small compared with the ultrasonic wavelength, experimental frequency-dependent backscatter coefficient data obtained by the participating laboratories were usually consistent with the expected Rayleigh scattering behavior (proportional to frequency to the fourth power). CONCLUSIONS: Greater standardization of backscatter measurement methods is needed. Measurements of frequency dependence of backscatter are more consistent than measurements of absolute magnitude.

1-Propanol↗

PubMed2004

Evaluation of unscanned-mode soft-tissue thermal index for rectangular sources and proposed new indices.

The monopole-source solution was used to calculate the three-dimensional complex acoustic pressure field for one-dimensional focused rectangular apertures in a medium having homogeneous acoustic and thermal properties. For each of six frequencies (1, 3, 5, 7, 9 and 12 MHz) and three focuses (f/1, f/2 and f/4), 33 rectangular aperture cases were investigated, for a total of 594 cases. For these focused field geometries, the three-dimensional temperature distribution was calculated using the bioheat transfer equation in homogeneous perfused media (attenuation = absorption: 0.3 dB/cm-MHz; perfusion length: 1.0 cm). For each of the 594 cases, the acoustic field was normalized to the derated spatial-peak temporal-average intensity (ISPTA.3) of 720 mW/cm2, the maximum value condition allowed based on the U.S. Food and Drug Administration (FDA) regulatory limit for most diagnostic ultrasound (US) equipment. Using the normalized acoustic field, the axial temperature increase profiles and the maximum temperature increases (DeltaTmax) were determined for each case. Also, from the normalized acoustic field, the unscanned-mode soft-tissue thermal index (TIS) for the rectangular sources was determined according to the procedures of the Standard for Real-Time Display of Thermal and Mechanical Indices on Diagnostic Ultrasound Equipment, commonly called the output display standard, ODS. The DeltaTmax:TIS ratio of the 594 cases yielded a mean value of 0.22, a median value of 0.16, a maximum value of 1.04 and a minimum value of 0.039. For all but one of the cases, TIS was greater than DeltaTmax. Also, two new unscanned-mode soft-tissue thermal indices (denoted TIS(new1) and TIS(new2)) were proposed. For new model 1, the DeltaTmax: TIS(new1) ratio yielded a mean value of 1.02, a median value of 1.01, a maximum value of 1.83 and a minimum value of 0.44. For new model 2, the DeltaTmax: TIS(new2) ratio yielded a mean value of 1.04, a median value of 0.99, a maximum value of 2.31 and a minimum value of 0.34. Further, both new models fit more closely to DeltaTmax than does the ODS-determined TIS and have the potential of being easier for manufacturers to implement because only the source power and frequency need to be measured.

Connective Tissue↗