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Biomedical subjects

William R Donaldson

Publications and source records attributed to William R Donaldson.

2 recordsLinked to original sources

Characterization of single and double fiber-coupled diffusing spheres.

A fiber-optic sensor, consisting of an optical fiber with and without a 1.59-mm-diameter spherical ceramic tip, inserted into a 19-mm-diameter Spectralon sphere has been characterized. This sensor is evaluated as a large-area omnidirectional sensor. An optical transport measurement system that rotates the sphere about two axes has been designed. The system measured the UV transport efficiency at 351 nm of light impinging on the sphere with an f-6 cone angle. When a bare fiber was placed at the center of the target sphere, the detection sensitivity was biased in the forward direction. The peak of the sensitivity of the inverse integrating sphere shifted from front to back as the bare fiber was withdrawn from the sphere and the numerical aperture of the fiber viewed more of the scattering volume. The response function with respect to the angle of incidence of the dual sphere was much more uniform than that obtained with a bare fiber in the center of the sphere.

Journal Article↗

Reverse intersystem crossing in rose bengal. II. Fluence dependence of fluorescence following 532 nm laser excitation.

A fluence-dependent fluorescence technique was used to observe reverse intersystem crossing from a certain higher-lying triplet state of rose bengal populated by a single pulse of 532 nm light. The quantum yield of reverse intersystem crossing from this state was determined to be 0.12+/-0.02 for rose bengal in phosphate-buffered saline. The importance of including molecular rotation effects in the analysis of fluorescence resulting from reverse intersystem crossing is discussed. Differences in the photochemical reactivity of upper triplet states in biological systems have been previously hypothesized to result from photophysical differences, particularly substantial differences in their reverse intersystem crossing yields. In this work this hypothesis is analyzed quantitatively, using numerical models of the population dynamics. These models suggest that reverse intersystem crossing alone cannot adequately explain the differences in biological response.

Fluorescence↗