Biomedical subjects

Wlad Kusnezow

Publications and source records attributed to Wlad Kusnezow.

3 recordsLinked to original sources

PubMed2003

Antibody microarrays: an evaluation of production parameters.

Antibody microarrays could have an enormous impact on the functional analysis of cellular activity and regulation, especially at the level of protein expression and protein-protein interaction, and might become an invaluable tool in disease diagnostics. The array surface is bound to have a tremendous influence on the findings from such studies. Apart from the basic issue of how to attach antibodies optimally without affecting their function, it is also important that the cognate antigens, applied within a complex protein mixture, all bind to the arrayed antibodies irrespective of their enormous variety in structure. In this study, various factors in the production of antibody microarrays on glass support were analysed: the modification of the glass surface; kind and length of cross-linkers; composition and pH of the spotting buffer; blocking reagents; antibody concentration and storage procedures, in order to evaluate their effect on array performance. Altogether, data from more than 700 individual array experiments were taken into account. In addition to home-made slides, commercially available systems were also included in the analysis.

Absorption↗

PubMed2002

Antibody microarrays: promises and problems.

Antibody microarrays have enormous potential for becoming a tool that will allow, at the protein level, the type of global characterization of molecular mixtures that DNA microarrays already make possible at the RNA and DNA level. However, the much higher complexity of proteins both in terms of their sheer number and their structural and biochemical diversity necessitates an even more sophisticated analysis process. Its eventual realization will be demanding to achieve and requires further developments on many technical aspects, not in the least because the understanding of proteins is still comparatively less comprehensive than that of nucleic acids prior to the emergence of array technologies.

Antibodies↗

PubMedDate not supplied

Solid supports for microarray immunoassays.

Stimulated by the achievements of the first phase in genomics and the resulting need of assigning functions to the acquired sequence information, novel formats of immunoassays are being developed for high-throughput multi-analyte studies. In principle, they are similar in nature to the microarray assays already established at the level of nucleic acids. However, the biochemical diversity and the sheer number of proteins are such that an equivalent analysis is much more complex and thus difficult to accomplish. The wide range of protein concentration complicates matters further. Performing microarray immunoassays already represents a challenge at the level of preparing a working chip surface. Arrays have been produced on filter supports, in microtiter plate wells and on glass slides, the last two usually coated with one-, two- or three-dimensionally structured surface modifications. The usefulness and suitability of all these support media for the construction and application of antibody microarrays are reviewed in this manuscript in terms of the different kinds of immunoassay and the various detection procedures. Additionally, the employment of microarrays containing alternative sensor molecules is discussed in this context. The sensitivity of microspot immunoassays predicted by the current analyte theory is not yet a reality, indicating the extent of both the technology's potential and the size of the task still ahead.

Acrylic Resins↗