Biomedical subjects

Wolfgang Hanke

Publications and source records attributed to Wolfgang Hanke.

5 recordsLinked to original sources

PubMed2005

Intrinsic optical signal of retinal spreading depression: second phase depends on energy metabolism and nitric oxide.

Spreading depression (SD) is a wave-like phenomenon that spreads through the gray matter of central nervous tissue. The aim of this work is to investigate how cellular energy supply and nitric oxide (NO) influence the recovery period after SD wave propagation. We have examined the SD wave in chicken retina by registration of the intrinsic optical signal (IOS). The changes of the IOS were observed via a microscope, transferred to a photomultiplier and amplified. The IOS of the SD wave consists of two phases. The first phase of IOS coexists with cellular swelling induced by ion distribution; the second phase is thought to reflect metabolic changes and reflects the refractory (recovery) period. To analyze the IOS, the amplitude, the duration and the front and the back maximal slopes of the both phases were analyzed. To reduce the cellular level of ATP the blocker of glucose transport-dexamethasone (glucocorticoid hormone) and the blocker of the respiratory chain-potassium cyanide were used. Sodium nitroprusside and trinitroglycerine were chosen as NO-donors. Our results show that during and after SD wave propagation (i) increased NO concentration changes the first and the second phases of IOS (duration of both phases is NO independent), (ii) reduced glucose uptake leads to an increased second phase duration and (iii) block of the respiratory chain prolongs the first phase. According to the results here presented, we propose that glycogen synthesis is one of the mechanisms reflected by the second phase of the IOS.

Analysis of Variance↗

PubMed2005

Propagation velocity and triggering threshold of retinal spreading depression are not correlated.

Spreading depression (SD) is a pronounced but transient disturbance of cellular homeostasis in the neuropil of the central nervous system which spreads in a wave-like manner across the tissue. At the wavefront the cells depolarize and a distinct ion redistribution between intra- and extracellular space is observed. In the aftermath of SD the recovering tissue is refractory: during an early absolute refractory period no further SD can be triggered, during the subsequent relative refractory period SD waves spread at lower velocity than usual. In this paper we shall examine the influence of temperature on SD triggering and on SD propagation in the chicken retina (retinal spreading depression, rSD) and we shall examine rSD triggering and rSD propagation in the refractory period. It will be shown that cooling decreases the threshold of rSD triggering, i.e. it becomes easier to trigger rSD when the temperature is reduced. At the same time cooling slows rSD propagation. In contrast, during the relative refractory period triggering rSD is more difficult than usual while rSD propagation is also slowed. These results demonstrate that the propagation velocity of rSD is not correlated with the triggering threshold. In particular, the propagation velocity of rSD must not be used to predict the influence of experimental conditions on the triggering threshold.

Animals↗

PubMed2004

Correlation between the durations of refractory period and intrinsic optical signal of retinal spreading depression during temperature variations.

Spreading depression (SD) is a neurophysiological phenomenon which occurs in the grey substance of the central nervous system. SD is characterised by a wave-like spread of depressed neuronal activity, by large ion shifts between intra- and extracellular space, by cellular depolarization, and by altered optical properties of the tissue giving rise to an intrinsic optical signal (IOS). In the shadow of SD further waves are difficult to trigger and such waves spread at lower velocity than usual. In this paper we examine the temperature dependence of the duration of this recovery (refractory) period and the temperature dependence of the duration of the IOS in the chicken retina. It is shown that these SD accompanying events are strongly dependent on temperature and that they are likely to depend on the metabolic rate in the tissue. The observed correlation of the duration of the IOS with the duration of the refractory period suggests that the IOS is a good indicator for the duration of the tissue recovery. Such a correlation would be of great value to the experimentalist who must know about the duration of the refractory period: while the latter is laborious to determine, recording the IOS is convenient.

Algorithms↗

PubMed2002

Gravity sensing in the central nervous system.

For human based space research it is of high importance to understand the influence of gravity on the properties of the central nervous system (CNS). Until now it is not much known about how neuronal tissue can sense gravity. The aim of this study was to find out weather and how the CNS, as a complex system, can percept and react to changes in gravity. Neuronal tissue and especially the CNS fulfils all the requirements for excitable media. Consequently, self-organisation, pattern formation and propagating excitation waves as typical events of excitable media have been observed in such tissue. The spreading depression (SD), an excitation depression wave is the most obvious and best described of these phenomena in the CNS. In our experiments we showed that the properties of the SD and therefore the CNS in its properties as an excitable medium reacts very sensitive to changes in gravity.

Journal Article↗

PubMed2002

Patch-clamp experiments under micro-gravity.

For human based space research it is of high importance to understand the influence of gravity on the properties of single ion channels in biological membranes, as these are involved in about all biological processes. The patch clamp technique is the best established method to investigate electrophysiological properties of single ion channels in detail. Consequently, a patch clamp set-up was designed for the drop tower in Bremen, Germany. Using this set-up among others, successfully leech neurons have been patched under micro-gravity, delivering data about ion channel behaviour, which were compared to results from bilayer experiments in the drop tower and to results from lab controls under 1 g and under higher gravity.

Journal Article↗