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Biomedical subjects

Wolfgang Osten

Publications and source records attributed to Wolfgang Osten.

At least 19 recordsLinked to original sources
PubMed2006

Complete wavefront reconstruction using sequential intensity measurements of a volume speckle field.

The recording of the volume speckle field from an object at different planes combined with the wave propagation equation allows the reconstruction of the wavefront phase and amplitude without requiring a reference wave. The main advantage of this single-beam multiple-intensity reconstruction (SBMIR) technique is the simple experimental setup because no reference wave is required as in the case of holography. The phase retrieval technique is applied to the investigation of diffusely transmitting and reflecting objects. The effects of different parameters on the quality of reconstructions are investigated by simulation and experiment. Significant enhancements of the reconstructions are observed when the number of intensity measurements is 15 or more and the sequential measurement distance is 0.5 mm or larger. Performing two iterations during the reconstruction process using the calculated phase also leads to better reconstruction. The results from computer simulations confirm the experiments. Analysis of transverse and longitudinal intensity distributions of a volume speckle field for the SBMIR technique is presented. Enhancing the resolution method by shifting the camera a distance of a half-pixel in the lateral direction improves the sampling of speckle patterns and leads to better quality reconstructions. This allows the possibility of recording wave fields from larger test objects.

Journal Article↗
PubMed2006

Chromatic confocal spectral interferometry.

Chromatic confocal spectral interferometry (CCSI) is a novel scheme for topography measurements that combines the techniques of spectral interferometry and chromatic confocal microscopy. This hybrid method allows for white-light interferometric detection with a high NA in a single-shot manner. To the best of our knowledge, CCSI is the first interferometric method that utilizes a confocally filtered and chromatically dispersed focus for detection and simultaneously allows for retrieval of the depth position of reflecting or scattering objects utilizing the phase (modulation frequency) of the interferometric signals acquired. With the chromatically dispersed focus, the depth range of the sensor is decoupled from the NA of the microscope objective.

Journal Article↗
PubMed2006

Simultaneous two-dimensional endoscopic pulsed digital holography for evaluation of dynamic displacements.

An endoscope is used in pulsed digital holography to simultaneously evaluate in-plane and out- of-plane transient and harmonic displacements on a flat metallic plate. The plate is illuminated from two different directions. The optical path for each illumination direction is matched to its corresponding reference beam, but also in such a way that each object-reference beam pair optical path is mismatched such that they are incoherent and can be stored in a single CCD frame. As is typical in these types of interferometric arrangement, two digital holograms are needed to compare two different states of the plate. Each hologram is Fourier transformed and due to the incoherence introduced, two separate spectra are readily identified, each belonging to an object-reference beam pair. On comparing by subtraction the phase obtained from the two pulsed digital holograms, it is possible to gather quantitative in-plane and out-of- plane results from transient and harmonic displacements.

Endoscopes↗
PubMed2006

Reconstruction algorithm for high-numerical-aperture holograms with diffraction-limited resolution.

A fast algorithm is proposed for the reconstruction of digital holograms that are recorded at high numerical aperture. The method directly evaluates the Rayleigh-Sommerfeld diffraction integral by use of a fast convolution algorithm. A shift parameter that accounts for the coordinate system's transverse displacement of the object plane and the hologram plane is introduced in a discrete representation of the diffraction kernel. Combination of the samplings reconstructed with different shift values yields diffraction-limited resolution over the full field of view. The algorithm is suitable for various applications such as holographic microscopy and metrology. Simulation and experimental results are presented.

Journal Article↗
PubMed2006

High-speed digital holographic interferometry for vibration measurement.

A system based on digital holographic interferometry for the measurement of vibrations is presented. A high-power continuous laser (10 W) and a high-speed CCD camera are used. Hundreds of holograms of an object that has been subjected to dynamic deformation are recorded. The acquisition speed and the time of exposure of the detector are determined by the vibration frequency. Two methods are presented for triggering the camera in order to acquire at a given phase of the vibration. The phase of the wavefront is calculated from the recorded holograms by use of a two-dimensional digital Fourier-transform method. The deformation of the object is obtained from the phase. By combination of the deformations recorded at different times it is possible to reconstruct the vibration of the object.

Journal Article↗
PubMed2006

Chromatically dispersed interferometry with wavelet analysis.

A new white-light interferometry point sensor utilizing a chromatically dispersed depth detection field is addressed. Monitoring the interference in the optical frequency domain allows for microscopic height detection without the necessity of a mechanical axial scan. The problem of limited dynamic range in previously reported spectral interferometric schemes is solved by forming a high-contrast interference window due to the chromatically dispersed focusing of the detection field. In a proof-of-principle experiment, the position of a reflecting object could be retrieved with a focus of 0.8 NA over an axial range of 30 microm by analyzing the phase of the emerging interference wavelets.

Journal Article↗
PubMed2006

Fast digital hologram generation and adaptive force measurement in liquid-crystal-display-based holographic tweezers.

Computer-generated holograms in conjunction with spatial light modulators (SLMs) offer a way to dynamically generate holograms that are adapted to specific tasks. To use the full dynamic capability of the SLM, the hologram computation should be very fast. We present a method that uses the highly parallel architecture of a consumer graphics board to compute analytical holograms in video real time. A precice characterization of the SLM (Holoeye LC-R-2500) and the adaption of its settings to our near-infrared application is necessary to guarantee an efficient hologram reconstruction. The benefits of a fast computation of adapted holograms and the application of an efficient SLM are demonstrated by measuring the trapping forces of holographic tweezers.

Journal Article↗
PubMed2006

Remote metrology by comparative digital holography.

A method for the remote comparison of objects with regard to their shape or response to a load is presented. The method allows interferometric sensitivity for comparing objects with different microstructure. In contrast to the well-known incoherent techniques based on inverse fringe projection this new approach uses the coherent optical wave field of the master object as a mask for the illumination of the sample object. The coherent mask is created by digital holography to allow instant access to the complete optical information of the master object at any place desired. The mask is reconstructed by a spatial light modulator (SLM). The optical reconstruction of digital holograms with SLM technology allows modification of reconstructed wavefronts with respect to improvement of image quality, the skilled introduction of additional information about the object (augmented reality), and the alignment of the master and test object.

Journal Article↗
PubMed2006

On-line surveillance of a dynamic process by a moving system based on pulsed digital holographic interferometry.

A method based on pulsed digital holographic interferometry for the measurement of dynamic deformations of a surface by using a moving system is presented. The measuring system may move with a speed of several meters per minute and can measure deformation of the surface with an accuracy of better than 50 nm. The deformation is obtained by comparison of the wavefronts recorded at different times with different laser pulses produced by a Nd:YAG laser. The effect due to the movement of the measuring system is compensated for by digital processing of the different holograms. The system is well suited for on-line surveillance of a dynamic process such as laser welding and friction stir welding. Experimental results are presented, and the advantages of the method are discussed.

Journal Article↗
PubMed2006

Optimally tuned spatial light modulators for digital holography.

Digital holography and comparative digital holography are applications that require computer-addressable modulators for the optical reconstruction of digital holograms. The quality of the reconstructed holograms depends on the modulator's properties. Therefore a characterization of the modulators is required. We show the result of a modulator characterization and the modulator's influence on the quality of the reconstructed hologram. We then compare qualitatively and quantitatively the optical reconstruction of phase and amplitude holograms by considering their level of detail and their diffraction efficiency.

Journal Article↗
PubMed2005

Applications of short-coherence digital holography in microscopy.

We present an optical system based on short-coherence digital holography suitable for the imaging of three-dimensional microscopic objects. The short temporal coherence properties of the light source allow optical sectioning of the sample. Proper reconstruction of different layers within biological samples is possible up to a depth of a few hundred micrometers, but multiple scattering and inhomogeneities in the refractive index reduce the imaging quality for deeper layers. We have studied the possibility of numerically correcting sample-induced aberrations, and we now propose a method of improving image quality. Numerical simulations and preliminary experimental results show that compensation of these aberrations is possible to some extent.

Algorithms↗
PubMed2005

Diffraction-induced coherence levels.

We examined the influence of complex diffraction effects on low-coherence fringes created for high-aspect depth-to-width ratio structures called trenches. The coherence function was analyzed for these micrometer-wide trenches and was registered with a white-light interference microscope. For some types of surface structure we observed that additional low-coherence fringes that do not correspond directly to the surface topology are formed near the sharp edges of the structures. These additional coherence fringes were studied by rigorous numerical evaluations of vector diffractions, and these simulated interference fields were then compared with experimental results that were obtained with a white-light interference microscope.

Computer Simulation↗
PubMed2004

Reconstruction of in-line digital holograms from two intensity measurements.

We propose a new method based on in-line digital holography for the reconstruction of a wave front from only two intensity recordings. The simulation result shows that this method works well when the object wave is weak compared with the reference wave. This technique can be employed for real-time imaging.

Journal Article↗
PubMed2004

Applications of fractional transforms to object reconstruction from in-line holograms.

We propose a method for the digital reconstruction of an object whose diffraction pattern has been recorded on a hologram. The fractional Fourier transform is used for the object reconstruction. To determine the position of the object, the fractional order is scanned. The fractional cosine and fractional Hartley transforms are also employed for object reconstruction. These two transforms are real valued and allow the reconstruction to be done with lower computing complexity. Simulations and experimental results are presented.

Journal Article↗
PubMed2003

Image reconstruction for in-line holography with the Yang-Gu algorithm.

A new approach to the numerical reconstruction of wave fronts stored by in-line holography is presented. The new algorithm can achieve good reconstructed results in both unitary and nonunitary systems. The influences of recording distance and noise as well as of digitalization errors on the quality of reconstruction are numerically investigated. The experimental results demonstrate the validity of this new approach.

Journal Article↗
PubMed2003

Temporal phase unwrapping of digital hologram sequences.

A method for recording and evaluating digital image-plane holograms is presented. Hundreds of holograms of an object that has been subjected to dynamic deformation (e.g., vibrations) are recorded. The phase of the wave front is calculated from the recorded holograms by use of a two-dimensional digital Fourier-transform method. By temporal phase unwrapping it is possible to determine the absolute deformation (included the direction of motion) of the object. Experimental results are presented, and the advantages of temporal phase unwrapping compared with spatial phase unwrapping are discussed.

Journal Article↗
PubMed2003

Miniaturized digital holography sensor for distal three-dimensional endoscopy.

A miniaturized sensor head for endoscopic measurements based on digital holography is described. The system was developed to measure the shape and the three-dimensional deformation of objects located at places to which there is no access by common measurement systems. A miniaturized optical sensor, including a complete digital holographic interferometer with a CCD camera, is placed at the end of a flexible endoscope. The diameter of the head is smaller than 10 mm. The system enables interferometric measurements to be made at speeds of as many as five reconstructions per second, and it can be used outside the laboratory under normal environmental conditions. Shape measurements are performed with two wavelengths for contouring, and the deformation is measured by digital holographic interferometry. To obtain full three-dimensional data in displacement measurements we illuminate the object sequentially from three different illumination directions. To increase the lateral resolution we use temporal phase shifting.

Endoscopes↗