Biomedical subjects

Wu Aihong

Publications and source records attributed to Wu Aihong.

3 recordsLinked to original sources

PubMed2005

Determination of trace silver by solid substrate-room temperature phosphorescence quenching method based on double catalytic system of meta-nitrophenyfluorone-polyoxyethylene-chromium-potassium bromate-beta-cyclodextrin.

A new solid substrate-room temperature phosphorescence (SS-RTP) quenching method for the determination of trace silver has been established. It is based on the fact that when using Mg(2+) as ion perturber and beta-CD as surfactant, the system of meta-nitrophenyfluorone (R)-polyoxyethylene-Cr(III) can emit strong and stable room temperature phosphorescence signal on filter paper whose surface is modified by polyvinyl alcohol (PVA)-H(3)BO(3)-NaOH. Ag(I) can catalyze KBrO(3) oxidizing R-PEO-Cr(III) system which causes the quenching of SS-RTP. The reducing value of phosphorescence intensity (DeltaI(p)) is directly proportional to the concentration of Ag(I) in the range of 3.2-160 ag spot(-1) (corresponding concentration: 2.43 fg ml(-1), the sample volume: 0.40 microl spot(-1)) with a detection limit (LD) of 0.97 ag spot(-1). The regression equation of working curve can be expressed as DeltaI(p)=13.92+0.3089m(Ag)+ (ag spot(-1)) (r=0.9983, n=6). This method has many advantages, such as a wide linear dynamic range, high sensitivity, good repeatability and selectivity. It has been applied to the determination of trace silver in real samples with satisfactory results. What is more, the mechanism of SS-RTP quenching method based on Ag(I) catalyzing KBrO(3) oxidizing meta-nitrophenyfluorone has also been discussed.

Catalysis↗

PubMed2005

Determination of human IgG by solid substrate room temperature phosphorescence immunoassay based on an antibody labeled with nanoparticles containing Rhodamine 6G luminescent molecules.

Luminescent silicon dioxide nanoparticles (R-SiO2) with size of 50 nm containing Rhodamine 6G (R) were synthesized by sol-gel method. In the presence of Pb(Ac)2 as a heavy atom perturber, the particle can emit intense and stable room temperature phosphorescence signal of R, respectively, on polyamide membrane, with the lambda(ex)(max)/lambda(em)(max) = 470/635 nm for R. Our research indicates that the specific immune reaction between goat-anti-human IgG antibody labeled with R-SiO2 and human IgG can be carried on polyamide membrane quantitatively, and the phosphorescence intensity was enhanced after the immunoreactions. Thus, a new method of solid substrate room temperature phosphorescence immunoassay (SS-RTP-IA) for the determination of human IgG was established basing on antibody labeled with the nanoparticles containing binary luminescent molecules. The linear range of this method is 0.0624-20.0 pg spot(-1) of human IgG (corresponding concentration, 0.156-50.0 ng mL(-1); sample volume, 0.40 microL spot(-1)). The regression equations of working curves are delta I(p) = 88.16. + 16.79 m(IgG) (pg spot(-1)) (485/646 nm, r = 0.9997). Detection limits calculated by 3Sb/k are 0.017 pg spot(-1). For samples containing 0.156 and 50.0 ng mL(-1) of IgG, we measured repeatedly for 11 times, RSDs are 3.9 and 2.8%, respectively. This method is sensitive, accurate and of high precision.

Antibodies↗

PubMed2003

Determination of trace tin by solid substrate-room temperature phosphorimetry using sodium dodecyl sulfate as sensitizer.

The effects of different surfactants on solid substrate-room temperature phosphorescence (SS-RTP) properties of Sn(4+)-morin systems were investigated. It was found that the SS-RTP intensity of luminescence system was increased greatly in presence of sodium dodecyl sulfate (SDS). A new highly sensitive method for the determination of trace tin has been proposed based on sensitization of SDS on SS-RTP intensity of morin-tin system on the filter paper substrate. The linear dynamic range of this method is 8.0-112 ag per spot (with the volume of 0.4 microl per spot) with a detection limit of 4.0 ag per spot, and the regression equation is DeltaIp=199.7+3.456m(Sn(IV)) (ag per spot), with the correlation coefficient r=0.9998 (n=7). This simple, rapid and reproducible method has been applied to determine the amount of tin in real samples with satisfactory results.

Luminescence↗