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Biomedical subjects

X B Hu

Publications and source records attributed to X B Hu.

5 recordsLinked to original sources
PubMed1990

[A simplified method for extracting and purifying bone morphogenetic protein].

Porcine bone morphogenetic protein was extracted and purified with general centrifugations and other biochemical techniques. Results showed that our methods were correct and reliable, which may cut down the costs of preparing BMP, simplify the procedure and improve the efficiency. Special polymerism by mesenchymal cells was observed during the early stage of inductive osteogenesis. The authors suggested this phenomenon probably be a sign which stood for the success of bone induction and a mark which the quality of inductive materials could be evaluated by.

Animals↗
PubMed1988

Separation and purification of porcine bone morphogenetic protein.

Porcine bone morphogenetic protein (pBMP) was separated and purified from porcine bone by repeated solubilization and precipitation of the protein with different concentrations of urea and GuHCl. The molecular weight of pBMP was determined to be 19.7 k. The property of inductive osteogenesis of pBMP caused new bone formation in muscular and subcutaneous tissues of strain No. 615 mice. Osteoid tissues were observed as early as ten days after implantation of pBMP, and lamellated new bones with marrow cavities were first found on the 15th day of implantation. A gradual transformation of mesenchymal-type cells into active chondroblasts and osteoblasts and finally into heterotopic new bone was observed and studied histologically and ultrastructurally.

Animals↗