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Biomedical subjects

X Jin

Publications and source records attributed to X Jin.

At least 181 records · Page 10Linked to original sources
PubMed1994

[Quantitative analysis of ephedrines in urine by HPLC].

Simultaneous determination of six ephedrines in urine sample has been achieved by high performance liquid chromatography on a Lichrospher RP-18 column, using methylamphetamine as internal standard. The 6 ephedrines are well separated in 25 minutes with resolution better than 1.8. This method has high recovery, selectivity and reproducibility, and the linearity is satisfactory from 1.5 micrograms/ml to 25 micrograms/ml with correlation coefficients better than 0.999.

Chromatography, High Pressure Liquid↗
PubMed1993

Quantitative analysis of the human immunodeficiency virus type 1 (HIV-1)-specific cytotoxic T lymphocyte (CTL) response at different stages of HIV-1 infection: differential CTL responses to HIV-1 and Epstein-Barr virus in late disease.

Major histocompatibility complex (MHC)-restricted cytotoxic T lymphocytes (CTL) are part of the cellular immune response to human persistent virus infections. Measurements of the frequency and specificity of human immunodeficiency virus type 1 (HIV-1)-specific CTL and their variation with time may indicate their relative importance in modulating the progression of HIV-1 infection. We have used limiting dilution analysis (LDA) to derive quantitative estimates of the frequency of HIV-1-specific CTL precursors in a cross-sectional study of 23 patients at different clinical stages of HIV-1 infection and to compare these with the frequency of CTL precursors specific for another persistent virus (Epstein-Barr virus [EBV]) in the same patients. Peripheral blood mononuclear cells (PBMC) were stimulated in vitro with autologous HIV-1-infected lymphoblasts and assayed for cytotoxicity in 51Cr release assays against autologous and MHC-mismatched lymphoblastoid B cells infected with recombinant vaccinia viruses expressing the three HIV-1 structural gene products. The frequency of MHC-restricted precursors was high in asymptomatic HIV-1-infected patients (env-specific CTL precursors up to 73/10(6) PBMC; gag-specific CTL precursors up to 488/10(6) PBMC), although the relative frequency against the different structural gene products varied from patient to patient. The HIV-1-specific CTL precursor frequency was reduced in patients who had more severe (< 400/microliters) CD4+ lymphocyte depletion, while in the majority of such patients the frequency of CTL precursors against EBV was maintained at levels observed in healthy controls. Direct CTL activity in unstimulated PBMC was observed in three of nine patients but no correlation was found between the presence of an activated CTL response and the magnitude of the CTL response detected after stimulation in LDA. Thus, CTL precursors were detected against all three HIV-1 structural gene products in patients with CD4+ lymphocyte counts > 400/microliters, at frequencies that are high compared with those reported for other persistent viruses. A CTL response directed against multiple protein antigens of HIV-1 may protect the patient against epitope variation. The fact that the EBV-specific CTL precursor frequencies were maintained in advanced HIV-1 infection suggests that there may be selective impairment of the HIV-1-specific CTL response associated with disease progression.

Adult↗
PubMed1993

[Studies on the pleomorphism of trachoma inclusion].

A series of trachoma inclusion color photograph is taken from the conjunctival smears of 48 trachoma cases. These smears were Giemsa-stained and stored in the slide-boxes for more than 30 years with no distinct changes in color. A description is given to the Pleomorphism of trachoma inclusion and its relation to the host cells. According to the gradual morphological changes of Chlamydia trachomatis multiplied in the infected cells, a 5-staged classification for trachoma inclusions is provided which may be benefit to understanding the pleomorphism of the trachoma inclusion and clinical laboratory diagnosis.

Chlamydia trachomatis↗
PubMed1992

[Solid-phase extraction and RP-HPLC screening procedure for diuretics, probenecid, caffeine and pemoline in urine].

A solid-phase extraction and reversed phase high performance liquid chromatographic method (RP-HPLC) was developed for the rapid determination of 13 diuretics (belonging to five different pharmacological groups), probenecid, caffeine and pemoline in urine. Two ml urine sample was first adsorbed on a XAD-2 column, then eluted with ether-ethyl acetate (1:1). The eluate was evaporated to dryness and reconstituted in methanol. The methanolic solution was injected into a HP LiChrosorb RP-18 column, using phosphate buffer (pH 3) and acetonitrile as the mobile phase and monitored at 216 nm, 230 nm, and 275 nm on a diode array ultraviolet detector. The extraction recoveries of 16 drugs were above 75%. The limits of detection ranged from 0.3-3.0 micrograms/ml of urine. All drugs were separately administered to healthy volunteers, positive urine samples were collected, and urinary excretion-time curves of some drugs were reported.

Adult↗
PubMed1991

[Thrombosis test in vitro of 107 cases with arteriosclerosis obliterans].

Thrombosis test in vitro of 107 cases with arteriosclerosis obliterans were measured and controlled by 36 normal persons. Of 107 cases, 13 cases belonged to ischemic phase, 47 cases nutritional disturbance phase, 47 necrosis (24 cases were first degree, 9 cases second degree, 14 third degree). Types distribution were: simple blood stasis 65 cases, blood and damp stasis 34, and 8 others. 46 patients were treated systematically by TCM-WM. The result proved that the indexes of thrombosis test in vitro of patients were much higher than that of normal persons (P less than 0.001). The relevant indexes were different in varied periods, degrees and types (P less than 0.05-0.01) and between before and after treatment (P less than 0.001). Of treated 46 cases, total effective rate was 86.96%, amputation rate was 13.04%. Formation of thrombus in vitro lowered 88.03%. The authors suggested that measurement of thrombosis test might be a accurate way to reflect the course of the disease and a basis for treatment and differentiation of symptoms.

Adult↗
PubMed1990

[Clinical analysis of 200 cases of necrotic thromboangiitis obliterans].

UNLABELLED: Two hundred cases of necrosis thromboangiitis obliterans (199 males, 1 females; age 17 approximately 63 years) were observed. The illness course averaged more than 5 years. Focuses of upper limbs were 6 cases and those of lower limbs 194 cases. 98 cases and 102 cases suffered from ulcer and gangrene respectively. 100 cases belonged to the first degree, 62 cases to the second degree, 38 cases to the third degree of III stage. 171 cases were treated by the combination of TCM and WM except 3 cases which were actively discharged and 26 cases which were to be given amputation. RESULTS: 90 cases within 171 cases were cured, 52 cases were improved obviously, 7 cases improved, 5 cases ineffective and 17 cases to be given amputation. The writers regard three principles should be followed in the treatment of necrosis thromboangiitis obliterans by the combination of TCM and WM. (1) The main importance was to improve blood circulation to remove blood stasis. (2) Controlling infection was the key point, effective antibiotics and hormone should be added besides these herbal medicines for clearing away heat and toxic materials. (3) General protecting therapy, performing various local operations on right period on the basis of improvement of blood circulation of the involved limbs, the wound could heal successfully. The writers suggest that the amputation should not be given above the level of the main artery obliterans.

Adolescent↗
PubMed1990

[A mathematical model of age distribution of malignant tumours mortality].

There was a tendency that the mortality of malignant tumours increased with age. In order to explore the law of this tendency, the data of malignant tumours mortality from disease monitoring points in Shandong Province were mathematically analogized, and the mathematical model of age distribution of malignant tumours mortality was established by using the exponential curve, y = 10a + bx. The model was also tested and verified by using national data, to observe the universal significance of the model. The model gave a theoretical account of the law of age distribution of malignant tumours mortality from a population, and provided an initial method to predict malignant tumours mortality. The increment quantity of malignant tumours mortality in various age groups when age increased one year could be calculated by using the differential equation from the exponential curve equation, y = 10a + bx. Further, "an increment multiple constant" of the increment quantity of malignant tumours mortality could be calculated. The constant could be used as an Index for comparison in risk degree and age distribution law of malignant tumours mortality among various age groups and the same age group in different periods, and provided leads for further research of the causes of malignant tumours.

Age Factors↗
PubMed1990

[Formation of extrinsic blood thrombosis in 102 cases of thromboangiitis obliterans].

The formation of extrinsic blood thrombosis in 102 cases of thromboangiitis obliterans and 54 healthy persons were observed with chandler method and their clinical manifestations. All the 102 patients were male, of which 13 cases were in ischemic stage, 45 cases in dystrophic stage, and 44 cases in necrotic stage. According to the differentiation of TCM, 8 belong to Yin-cold type, 50 belong to downward flowing of damp-heat type, 42 belong to blood stasis type, and 2 others. Combining method of TCM-WM was adopted to give 45 cases systemic treatment, that is, taking the promotion of blood flow to remove its stasis as the dominant factor, accompanied by intravenous drip and operation. Results showed that the length, wet weight and dry weight of extrinsic blood thrombosis were higher apparently in the cases of thromboangiitis obliterans than those in healthy persons (P less than 0.001), and there were significant differences between the values of extrinsic blood thrombosis in different stages and different degrees. After systemic treatment with TCM-WM, the values of extrinsic blood thrombosis decreased remarkably, and their symptoms and signs improved or disappeared. The clinical total effective rate was 88.89%, and the decreasing rate of the values of extrinsic blood thrombosis was 98.9%. This suggested that the high blood coagulation and extrinsic thrombosis should draw special attention and may act as an approach reflecting the severity of the disease and evaluating the effects of treatment.

Adolescent↗