Biomedical subjects

X Lou

Publications and source records attributed to X Lou.

36 records · Page 2Linked to original sources

PubMed1999

Lymphocyte DNA damage in cigarette factory workers measured by the Comet assay.

To investigate whether there were separate and combined effects of occupational exposure to tobacco dust and smoking on lymphocyte DNA damage, 148 workers from a cigarette manufacturing factory (107 occupationally exposed to tobacco dust from the production department and 41 unexposed controls who were managerial workers) were included in the study. The Tail Moment (TM) of Comet assay was used to measure DNA damage. The two groups had similar mean age, mean duration of work and smoking prevalence. The exposed workers had a larger TM than that of the controls (mean+/-S.D.: 43.43+/-13. 77 vs. 38.89+/-8.98, p<0.05). Smokers had significantly larger TM than non-smokers (47.25+/-14.02 vs. 38.90+/-10.75, p<0.001). Analysis of variance after adjustment for age and gender showed that occupational exposure and smoking had a significant and independent effect on Tail Moment (p=0.025 and p=0.002, respectively) and there was a significant positive two way interaction between the two factors (p=0.019). Age and gender had no significant effect on TM. The present study suggests that smoking and tobacco dust exposure can induce lymphocyte DNA damage and there is a synergistic effect of tobacco dust exposure and smoking on DNA damage.

Adult↗

PubMed1999

Ca2+ response of rat mesangial cells to ATP analogues.

The aim of this investigation was to characterise the effects of ATP analogues and UTP on the single cell intracellular Ca2+ concentration ([Ca2+]i) in cultured rat mesangial cells. Typically, there were two phases in the Ca2+ response to the agonists, an initial fast transient peak and a subsequent slower decline, or plateau, phase. For the peak amplitude in [Ca2+]i the agonists had about equal effect. But when taking in consideration the percentage of responding cells and the integrated Ca2+ response over 1 min, the order of efficacy of nucleotide agonists (100 microM) was UTP = ATP > ATPgammaS > ADP = 2MeS-ATP (2-methylthio-ATP). Adenosine, AMP and beta,gamma-Me-ATP (100 microM) had no effect. Suramine (100 microM) and reactive blue (50 microM) decreased the number of responding cells. Removing Ca2+ from the bath diminished neither the peak in [Ca2+]i nor the percentage of responding cells, but the average [Ca2+]i increase in 1 min was significantly reduced. The results indicate that P2Y2 receptors are present in rat mesangial cells but it cannot be excluded that there are receptors distinct from P2Y2 which also mediate a rise in [Ca2+]i.

Adenosine↗

PubMed1999

Swelling behavior and mechanical properties of chemically cross-linked gelatin gels for biomedical use.

Cross-linked gelatin gels are used as biomaterials in living tissues, either as bioadhesives or as devices for sustained drug release. As these applications involve surgical insertion of gels, the effect of cross-linking on mechanical properties is relevant. The effect of cross-linking on the gel water uptake is also relevant for the kinetics of drug release. Equally important is the influence of initial amounts of gelatin in the gelling solutions. We investigated the influence of cross-linking (with glutaraldehyde) and of gelatin content upon the equilibrium water content and tensile properties of resulting gels. A range of gels was prepared with gelatin contents of 10 to 40% wt, and cross-linked with 2.5 to 50% wt glutaraldehyde. The increase in gelatin content and degree of cross-linking reduced the water uptake of gels from about 60-65% wt to about 50% wt. The tensile characteristics were differentially affected. While the increase of cross-linking induced a decrease of toughness and elasticity and an increase of stiffness, it did not affect the ultimate strength of gels. On the contrary, the increase of gelatin content induced a definite increase of the ultimate strength but did not significantly affect the other properties.

Biocompatible Materials↗

PubMed1999

Standard growth curve chart for body height of Chinese adolescent for orthodontic diagnosis.

OBJECTIVE: To make a standard growth curve chart on the body height of Chinese adolescents. METHODS: Statistical data from 32,524 Sichuan students aged 7 to 19 years were surveyed in 1995. Mean values of the annual increment of body height and the ages of the pubertal growth periods were analyzed. RESULTS: During the period between 9 and 13 years of age, females grow faster than their male peers. After age 13 years, males surpass females, and the difference in physical growth and development becomes more pronounced. CONCLUSIONS: The new standard growth curve chart on the body height of Chinese adolescent may be applied to better understand the growth pattern of Chinese orthodontic patients.

Adolescent↗

PubMed1999

[Clinical study on treatment of rectal carcinoma with Chinese herbal medicine and high dose fluorouracil emulsion via rectal infusion].

OBJECTIVE: To study the clinical significance of rectal infusion of Chinese herbal medicine (CHM) plus high dose fluorouracil emulsion in treating rectal carcinoma. METHODS: The 86 patients of rectal carcinoma were randomly divided into CHM plus chemotherapy group and single chemotherapy group, and the changes of T-lymphocyte cell subset, natural killer (NK) activity, pathologic picture and clinical symptoms were analysed statistically before and after treatment. RESULTS: In the single chemotherapy group, the CD3 cell and NK activity decreased after treatment and most of the peri-carcinoma cellular infiltration were in grade I. In the CHM plus chemotherapy group, the CD3, CD4/CD8 and NK activity restored and CD8 lymphocyte decreased markedly after treatment in comparing with those before treatment (P < 0.05, P < 0.01), and most of the peri-carcinoma cellular infiltration concentrated in grade III. CONCLUSION: Chemotherapy alone could suppress cellular immune function significantly, significant immunoregulation and improvement of symptoms (as mucous-bloody stool, frequent defecation) could be obtained by combination therapy of CHM and chemotherapy.

Adenocarcinoma↗

PubMed1999

[Studies on standard growth curve chart on body height of Chinese adolescent for orthodontic diagnosis].

OBJECTIVE: To make a standard growth curve chart on the body height of Chinese adolescent. METHODS: Statistical data were surveyed in 1995, which were obtained from subjects consisting of 32,524 students aged from seven to nineteen years in the province of Sichuan. RESULTS: Mean values of the annual increment of body height and the ages of the pubertal growth periods were analyzed. CONCLUSION: The new standard growth curve chart on the body height of adolescent chinese were applied for better understanding the growth pattern of chinese orthodontic patients.

Adolescent↗

PubMed1998

GIPC, a PDZ domain containing protein, interacts specifically with the C terminus of RGS-GAIP.

We have identified a mammalian protein called GIPC (for GAIP interacting protein, C terminus), which has a central PDZ domain and a C-terminal acyl carrier protein (ACP) domain. The PDZ domain of GIPC specifically interacts with RGS-GAIP, a GTPase-activating protein (GAP) for Galphai subunits recently localized on clathrin-coated vesicles. Analysis of deletion mutants indicated that the PDZ domain of GIPC specifically interacts with the C terminus of GAIP (11 amino acids) in the yeast two-hybrid system and glutathione S-transferase (GST)-GIPC pull-down assays, but GIPC does not interact with other members of the RGS (regulators of G protein signaling) family tested. This finding is in keeping with the fact that the C terminus of GAIP is unique and possesses a modified C-terminal PDZ-binding motif (SEA). By immunoblotting of membrane fractions prepared from HeLa cells, we found that there are two pools of GIPC-a soluble or cytosolic pool (70%) and a membrane-associated pool (30%). By immunofluorescence, endogenous and GFP-tagged GIPC show both a diffuse and punctate cytoplasmic distribution in HeLa cells reflecting, respectively, the existence of soluble and membrane-associated pools. By immunoelectron microscopy the membrane pool of GIPC is associated with clusters of vesicles located near the plasma membrane. These data provide direct evidence that the C terminus of a RGS protein is involved in interactions specific for a given RGS protein and implicates GAIP in regulation of additional functions besides its GAP activity. The location of GIPC together with its binding to GAIP suggest that GAIP and GIPC may be components of a G protein-coupled signaling complex involved in the regulation of vesicular trafficking. The presence of an ACP domain suggests a putative function for GIPC in the acylation of vesicle-bound proteins.

Adaptor Proteins, Signal Transducing↗

PubMed1998

Biodegradation in vitro and retention in the rabbit eye of crosslinked poly(1-vinyl-2-pyrrolidinone) hydrogel as a vitreous substitute.

To elucidate the relatively short retention of crosslinked poly(1-vinyl-2-pyrrolidinone) hydrogels in the eye when used as potential vitreous substitutes, a 14C-labeled hydrogel was produced and subjected to both in vitro biodegradation assays and in vivo experiments. The polymer was synthesized by the free-radical copolymerization of 99% 1-vinyl-2-pyrrolidinone with 1% 14C-methyl methacrylate in the presence of ethylene glycol dimethacrylate (0.1%) as crosslinking agent. The in vitro protocol for assessing the biodegradation included the incubation of hydrogel with hydrolases (trypsin or collagenase), followed by examination of changes in its physical characteristics and by monitoring its residual radioactivity, as well as by detection of possible degradation products. Within the maximum duration of experiments (4 weeks), none of the procedures indicated biodegradation of polymer. The hydrogel was also injected into the vitreous humor of rabbits and followed up to 4 weeks. Residual radioactivity measurements of the vitreous contents indicated that 50% of the polymer was removed by the end of this period. Histopathologic examination revealed cell infiltrates of the mononuclear phagocyte system in both vitreous and retinal tissue. A possible phagocyte-mediated mechanism for the dissipation of hydrogel is discussed.

Animals↗

PubMed1998

Clinical results of implantation of the Chirila keratoprosthesis in rabbits.

AIMS/BACKGROUND: An ideal keratoprosthesis (KPro) would closely resemble a donor corneal button in terms of its surgical handling, optics, and capacity to heal with host tissue in order to avoid many of the complications associated with the KPros which are currently in clinical use. This study was carried out to assess the long term clinical outcomes on implantation of the core and skirt poly(2-hydroxyethyl methacrylate) KPro in animals. METHODS: 20 KPros were made and implanted as full thickness corneal replacements into rabbits and followed for up to 21 months to date. RESULTS: 80% of the prostheses have been retained, with a low incidence of complications such as cataract, glaucoma, and retroprosthetic membrane formation which are frequently associated with KPro surgery. CONCLUSIONS: KPros of this type may offer promise in the treatment of patients for whom penetrating keratoplasty with donor material carries a poor prognosis. Refinement of the KPro and further animal trials, including implantation into abnormal corneas, are however mandatory before human implantation could be planned.

Animals↗

PubMed1998

[The development of SH-C1 autobiochemical analyzer].

As a miniature automatic analyzer, SH-C1 analyzer can be divided into five parts: the diluter, the circular reaction plate, the photometer, the electronic control board and the main microcomputer system with CRT monitor. The paper discusses its general structure and its key parts: the double-beam high precision photometer and the diluter controlled by microprocessors. The paper also describes the software system and the properties of the instrument.

Autoanalysis↗

PubMed1998

[Sequencing of a beta-amylase gene from Bacillus firmus].

The gene encoding a beta-amylase from Bacillus firmus 725 was sequenced. The sequenced DNA of 2012 bp contains one open reading frame of 1406 nucleotides without a translation stop codon. The deduced amino acid sequence homology with those known bacterial and some plant beta-amylase was 98% for Bacillus polymyxa 72, 98% for Bacillus polymyxa ATCC8523, 82% for Bacillus circulans, 54% for Clostridium thermosulfurogenes, 49% for Bacillus cereus BQ10-S1, 50% for Bacillus cereus var. mycoides, 36% for barley, and 36% for soybean Eleven well-conserved regions were found among the amino acid sequences of the nine beta-amylases.

Amino Acid Sequence↗

PubMed1998

Altered irinotecan and SN-38 disposition after intravenous and oral administration of irinotecan in mice bearing human neuroblastoma xenografts.

The antitumor activity of irinotecan in vitro primarily results from its hydrolysis by carboxylesterase to the active metabolite SN-38. The present study was conducted to evaluate the effect of human neuroblastoma xenografts on irinotecan and SN-38 disposition after i.v. and oral irinotecan administration. Non-tumor-bearing mice and mice bearing three different human neuroblastoma xenograft lines (NB1691, NB1643, and NBEB) were given irinotecan (10 mg/kg) by short i.v. injection into the tail vein or by oral gavage. Serial plasma samples were obtained, processed to isolate irinotecan and SN-38 lactone, and assayed with a sensitive and specific high-performance liquid chromatography assay. Noncompartmental and compartmental pharmacokinetic analyses were performed. A four-compartment model was used for analysis of irinotecan and SN-38 concentration-time data after i.v. administration. The presence of tumor increased irinotecan systemic exposure (1.2-3.8-fold; P < 0.05) after i.v. and oral administration in mice bearing neuroblastoma xenografts compared to non-tumor-bearing mice. Moreover, SN-38 systemic exposures were higher (1.3-3.8-fold; P < 0.05) in mice bearing human neuroblastoma xenografts as compared to non-tumor-bearing mice, with the greatest effect observed after oral administration of irinotecan. A schematic model is presented to provide a mechanistic basis for our observations. These results emphasize the need to perform preclinical pharmacokinetic studies to evaluate the influence of tumor on drug disposition.

Administration, Oral↗

PubMed1997

Expression and functional domains of rabbit liver UDP-glucuronosyltransferase 2B16 and 2B13.

Southern blot analysis has demonstrated that the 5' portion of the rabbit liver dexamethasone-inducible UDP-glucuronosyltransferase (UGT) 2B13 RNA is related in sequence to a family of UGT genes (Tukey, R. H., Pendurthi, U. R., Nguyen, N. T., Green, M. D., and Tephly, T. R. (1993) J. Biol. Chem. 268, 15260-15266). To identify these additional gene transcripts, rabbit liver cDNA libraries were screened with a 5' conserved 330-base pair UGT2B13 cDNA fragment, resulting in the isolation and characterization of several rabbit liver UGT cDNAs. One such clone, called pGT11, encodes a putative glycoprotein that is 78% similar to rabbit UGT2B13. The new UGT has been designated UGT2B16. The UGT2B16 gene is expressed as a single 4200-base RNA transcript that is regulated only in adult rabbits. The predicted NH2-terminal 25 amino acids of UGT2B16 are identical to that of rabbit liver UGT2B13, with the remainder of the protein being 77% similar to UGT2B13. Expressed UGT2B16 protein in COS-1 cells was active toward 4-hydroxybiphenyl, similar to that of UGT2B13. However, UGT2B16 efficiently conjugated 4-hydroxyestrone and 4-tert-butylphenol, substrates that are not efficiently catalyzed by UGT2B13. To further characterize the structural domains of UGT2B16 and UGT2B13, a series of chimeric cDNAs were constructed that contained portions of both UGT2B16 and UGT2B13. Chimeric 2B163002B13531, which contained the amino-terminal UGT2B16 amino acids 1-300 followed by amino acids 301-531 of UGT2B13, as well as chimeric 2B163582B13531 and 2B164342B13531 proteins, catalyzed the glucuronidation of 4-hydroxyestrone, indicating that the carboxyl terminus of UGT2B13 could substitute for those same regions on UGT2B16. However, the replacement of the carboxyl end of UGT2B13 with 2B16300-531 or 2B16434-531 dramatically impaired the catalytic function of the chimeric proteins. These results indicate that the carboxyl end of UGT2B13 plays an important role in the functional and possible conformational state of the protein.

Amino Acid Sequence↗

PubMed1997

Keratoprosthesis results in animals: an update.

BACKGROUND: The report presented is an update on continuing development work on modified PHEMA core-and-shirt KPros in animals. METHODS: Two variations (improved wet-eye, and dry-eye) of a prototype core-and-skirt Chirila KPro are described. The clinical success rate on implantation of these versions of the Chirila KPro was assessed. RESULTS: It was found that a significant improvement in retention rate was shown in the improved model but that the dry-eye model failed early in two of the three implanted. CONCLUSIONS: The significance of the improved strength and the reasons for disappointing results with the early dry-eye KPros are discussed. Ongoing work is briefly outlined.

Animals↗

PubMed1997

Histologic evaluation during healing of hydrogel core-and-skirt keratoprostheses in the rabbit eye.

PURPOSE: We developed two models that are modifications of our original poly(2-hydroxyethyl methacrylate) (PHEMA) core-and-skirt keratoprosthesis. In these keratoprostheses, the mechanical strength of the skirt has been considerably increased with divinyl glycol (DVG) as a cross-linking agent during polymerization. In one (KPro I), methyl methacrylate (MMA) was added as comonomer to increase cell adhesion, and in the other (KPro II), HEMA was polymerized with DVG without comonomer. The aim of this study was to evaluate the process of healing and biocolonization and to ascertain whether KPro I demonstrates better ingrowth than the mechanically stronger KPro II, after implantation in rabbit eyes. METHODS: Ten rabbits were used for each model and studied at five predetermined end points up to 26 weeks. The device was implanted as a full-thickness keratoprosthesis covered with a conjunctival flap. RESULTS: Neither prosthesis demonstrated extrusion or retroprosthetic membrane formation. There was no significant difference between the two types of prosthesis with respect to tissue ingrowth and surrounding tissue melting. Histologically, inflammation was not severe, but calcification was seen in most specimens. Evidence of biodegradation of the prosthesis also was seen. CONCLUSION: In our original keratoprosthesis, fibrovascular invasion had occurred into the prosthetic skirt, but wound dehiscence and low mechanical strength resulted in an unfavorable outcome. In this series, the mechanical properties were improved, and KPro II was stronger than KPro I. Therefore KPro II would be the preferred polymer combination for surgical manipulation. However, biodegradation and calcification require further investigation into the degree and significance of these adverse reactions.

Animals↗

PubMed1995

[Placental tissue histomorphometry in hydrops fetalis].

Placentae from 10 hydrops fetalis with hemoglobin Bart's disease were studied by tissue volumetry on the relative percentage of placental proper to its components, the surface area of villi and villi capillaries, and by comparing with 6 placentae from premature deliveries of 30-36 weeks. The placental weight, volume, percentage of placental proper and the villi volume, as well as the surface area of villi were significantly increased as compared with the controls. The relatively decreased volume percentage of intervillous space volume and surface area of villi capillaries resulted in a reduction of maternal-fetal exchanging surface area. We suggest careful prenatal scanning for Thalassemia and avoiding marriage between local peoples to reduce the incidence of hemoglobin Bart's disease.

Anthropometry↗

PubMed1994

[A case-control study on risk factors in etiology of Parkinson's disease].

Risk factors of Parkinson's disease (PD) were studied by the case-control method (1:1) in 90 cases. It was suggested that the high positive rate (34.4%) in PD family history was related to "genetic susceptibility". There was history of head injury, rheumatic fever, seizure, taking anti-psychosis drug or contact with toxins, but any one factor could not completely explain the etiology of PD. We believe that coordinating effects of both susceptibility in genetics and environmental toxins may explain the mechanism of PD.

Aged↗

PubMed1993

Coordinate and noncoordinate regulation of synaptic vesicle protein genes during embryonic development.

Because the formation of a mature nerve terminal requires the accumulation of large quantities of synaptic vesicles, the expression of synaptic vesicle proteins would be expected to correlate with synaptogenesis. However, previous studies have provided conflicting evidence on this point. We have examined the developmental pattern of expression of mRNA and protein for three RNAs derived from two genes coding for synaptic vesicle membrane proteins. For these experiments, we cloned a chick p65 (synaptotagmin) cDNA using a reduced stringency screen with a rat p65 cDNA probe. We examined p65 expression in chick forebrain in conjunction with that of synaptophysin II. RNase protection assays for p65 and the two isoforms of synaptophysin II (Bixby, 1992) show essentially coordinate increases of these three mRNAs in embryonic forebrain during the peak period of synaptogenesis (E17 to E20). However, each of the three mRNAs has a distinct temporal pattern of expression during the early stages of embryogenesis. In the ciliary ganglion, upregulation of synaptophysin II mRNA correlates very well with synaptogenesis. Our results suggest that the regulation of expression of vesicle membrane protein mRNA can serve as a marker for synaptogenesis, despite temporal differences in early expression patterns. In contrast to mRNA expression, assays for vesicle protein expression show a relatively steady rise in both p65 and synaptophysin II throughout the embryonic period, without a sharp increase corresponding to that seen in message levels. These results suggest that the expression both of the p65 and of the synaptophysin II proteins is post-transcriptionally regulated.

Amino Acid Sequence↗