Biomedical subjects

Xiajing Tang

Publications and source records attributed to Xiajing Tang.

3 recordsLinked to original sources

PubMed2006

Expression of Hsp70 and Hsp27 in lens epithelial cells in contused eye of rat modulated by thermotolerance or quercetin.

PURPOSE: To investigate the dynamic expression of heat shock protein (Hsp)70 and Hsp27 in lens epithelial cells (LECs) of contused eyes and the effects of heat shock and quercetin. METHODS: Rat eyes were randomized into three groups: contusion, heat shock, and quercetin. Eye contusions were induced by dropping a 20 g steel ball from a height of 20 cm for 100 times. Heat shock was induced by raising the core body temperature to 40.5-41.5 degrees C for 8 min, 2-3 h before eye contusion. Quercetin, an inhibitor of Hsp expression, was given via gavage (100 mg/kg) 2-3 h before eye contusion. Experimental eyes were enucleated at 0, 1, 3, 6, and 24 h following each group treatment for the detection of Hsp70 and Hsp27 expression in LECs using RT-PCR. Lens sections were examined by immunohistochemistry with antibodies to Hsp70. RESULTS: Basic expression of Hsp70 and Hsp27 was observed in LECs. Expression of Hsp70 in LECs was increased after contusion in a time-dependent manner. Preconditioning hyperthemia (45 degrees C, 8 min) resulted in a significant increase of Hsp70 expression compared to the base level, and enhanced the expression in each subgroup in contrast to the corresponding subgroup in the contusion group. However, Hsp70 levels in the 1-6 h subgroup in the quercetin group was significantly lower. There was no notable change of expression of Hsp27 between each group. Immunostaining of Hsp70 was prominent in LECs of contusion eyes and heat shock eyes compared with those in normal eyes. Immunostaining of Hsp70 was faint in LECs of quercetin eyes. CONCLUSIONS: Increased expression of Hsp70 in LECs of contused eyes may play a protective role against degeneration of lens protein. Thermal preconditioning possibly protects against lens injury by increasing the expression of Hsp70. Endogenous protective mechanisms may be important in eye contusion.

Animals↗

PubMed2006

Clinical evaluation using Custom Control Software technology in coaxial phacoemulsification.

PURPOSE: The aim of this study was to evaluate the clinical outcomes of the Custom Control Software technology in coaxial phacoemulsification. METHODS: This prospective, randomized, double-masked clinical trial was conducted at the Eye Center, Affiliated Second Hospital, College of Medicine, Zhejiang University. The patients were grouped according to nuclear sclerosis and randomly assigned to undergo conventional phacoemulsification or phacoemulsification using Millennium Custom Control Software (CCS). All surgeries were performed by a single surgeon. The phaco time and average power of phacoemulsification were recorded and then calculated as absolute phaco time (APT). Visual acuity, pachymetry, endothelial cell density and anterior chamber flare count of both groups were noted at preoperatively day 1 and 3 months after surgery. RESULTS: The APT of the CCS group was statistically lower than Conventional group in grades II, III and IV (P < 0.05) nuclear sclerosis, but the two groups had no statistically significant differences in the grade I group. At day 1 post surgery, there were significant differences in the thickened pachymetry (P = 0.047) and anterior chamber flare counts (P = 0.008) between two groups. At 3 months post surgery, the endothelial cell loss ratio showed statistical difference (P = 0.032) between two groups. CONCLUSIONS: Using CCS technology in coaxial phacoemulsification can provide lower APT and better power efficiency. This new power modulation may make the surgery safer and more effective than Conventional phacoemulsification.

Aged↗

PubMed2005

Progressive polymorphic congenital cataract caused by a CRYBB2 mutation in a Chinese family.

PURPOSE: To report and identify the genetic defect that causes progressive polymorphic congenital cataracts affecting a large five generation Chinese family. METHODS: Family history and phenotypic data were recorded, and the phenotypes were documented by slit lamp photography. Genetic linkage analysis was performed on the known genetic loci for autosomal dominant congenital cataract (ADCC) with 41 short tandem repeat polymorphic markers. Mutations were screened by DNA sequencing and restriction fragment length analysis (RFLP). RESULTS: A significant two point LOD score was generated at marker D22S420, D22S539 and D22S315 for 22q11.2. The highest observed LOD score was 6.26 (theta=0.00) with marker D22S315. Mutation screening of the CRYBB2 gene in this family revealed an C-->T transition at position 475 (Q155X) of the cDNA sequence, creating a novel SpeI restriction site that cosegregated with affected members of the pedigree, but was not present in unaffected members or any of the 100 unrelated individuals tested. CONCLUSIONS: Our finding expands the spectrum of cataract phenotypes caused by the Q155X mutation of CRYBB2, confirms the phenotypic heterogeneity of this mutation and suggests the mechanism that influences the congenital cataract formation in different ethnic backgrounds.

Adult↗