Biomedical subjects

Xiang Huang

Publications and source records attributed to Xiang Huang.

23 records · Page 2Linked to original sources

PubMed2003

[Circadian rhythms of DNA synthesis and telomerase expression in hepatic cancer transplanted in nude mice].

OBJECTIVE: To study the circadian rhythms of DNA synthesis and telomerase expression in hepatic cancer transplanted in nude mice. METHOD: Sixteen BALB/C mice were synchronized with an alternative lighting regimen with 12 h for light and 12 h for darkness (12:12 LD) for 4 weeks. Hepatic cancer cells (SMMC-7721) were implanted into both flanks of each mouse. One week after transplantation, sampling from the tumor was conducted at 3, 9, 15 and 21 h after light onset (HALO). Single cell suspension was obtained and stained with propidium iodide. The cellular DNA content was measured by flow cytometry. Telomerase activity was measured by PCR-ELISA assay. Data were documented by ANOVA and Cosinor analysis. RESULT: The proportion of tumor cells in phase G1, S, G2/M and telomerase activity varied according to circadian time with statistical significance, and the telomerase activity showed a synchronized variation. The distribution curves of both phase S and the expression level of telomerase were fit for Cosinor changes. CONCLUSION: DNA synthesis and telomerase expression of SMMC-7721 cells transplanted into the nude mice varies according to the circadian rhythm. The results provide a guidance for laying down the chemotherapy protocol for human tumors, especially when the telomerase inhibitor was used as the anti-cancer agent.

Animals↗

PubMed2002

[Cultivation of fibroblasts derived from urethral scar and the effects of salviae miltiorrhizae and ligustrazine on its growth].

OBJECTIVE: To explore the possible effect and related mechanism of salviae miltiorrhizae (SM) and ligustrazine in the treatment of urethral scar. METHODS: In vitro cultivation of fibroblasts derived from urethral scar has been developed. The effects of these two medicines on its morphology and proliferation have been detected based on the in vitro culture system. RESULTS: The spindle cells were found to become elliptical in shape. The two medicines inhibited the proliferation of fibroblasts derived from urethral scar, and this may be resulted from their inhibitive effects on cell division rather than cell necrosis or degradation. CONCLUSION: Salviae multiorrhizae and ligustrazine as effective scar inhibitors may have application prospects in the treatment of urethral stricture.

Adult↗

PubMed2002

[Correlation between expression of Fas protein and hair cell apoptosis in basilar papilla of chicken].

OBJECTIVE: To study the relationship between expression of Fas protein and hair cell apoptosis following drug ototoxicity in the chicken inner ear. METHOD: The damaged chicken inner ear model was developed by a subcutaneous injection of kanamycin at a daily dose of 100 mg/(kg.d) for 10 consecutive days. The Fas protein positive cells and apoptotic cells were examined by immunohistochemistry and TUNEL method at 1, 3, 7, 14, 21 days post-treatment. RESULT: TUNEL positive cells were localized to luminal layer of the sensory epithelia in the basilar papilla, there were much more apoptosis cells at the seventh day post-treatment. Fas positive cells began to increase at the first day post-treatment, and expression level of Fas protein increased to its peak at the third day after the last injection. At the fourteenth day post-treatment, TUNEL positive cells decreased following a low expression of Fas protein in the basilar papilla. CONCLUSION: There is a relationship between hair cell apoptosis and high expression of Fas protein in chicken inner ear following chronic kanamycin ototoxicity, Fas protein may contribute to regulate and control hair cells apoptosis.

Animals↗

PubMed2002

[Audiological characteristics of auditory neuropathy].

OBJECTIVE: To study the auditory electrophysiological characteristics of the auditory neuropathy. METHODS: Ten patients were diagnosed as auditory neuropathy. The history, pure tone audiometry, stapedial reflex, auditory brainstem response, electrocochleogram, distortion product otoacoustic emission and his contralateral suppression of white noise, middle latency response and slow cortical response were assessed. Five patients were investigated with brain CT or MRI. RESULTS: The patients were of both genders. The average age was 20.3 years old and most of them were teenagers. Nine patients complained of bilaterally hearing impairment, while one patient who did not complain of hearing loss had a tinnitus of both ears. The pure tone audiogram showed a middle or moderate sensorineural hearing loss at low frequencies 0.5 kHz and/or 0.25 kHz in nineteen ears but a normal audiogram in one ear. Stapedial reflex was absent in nineteen ears, and threshold elevated in one ear while his pure tone audiogram revealed an ascending curve. Auditory brainstem response cannot be recorded or only wave V or/and wave I presented. All of the patients were evoked a distortion product OAE, which cannot be suppressed by contralateral white noise. Electrocochleogram demonstrated AP absent or very small amplitude in most ear and the--SP amplitude were 0.595 microV, while they had a normal slow cortical response but except one patient presented normal middle latency response. The findings of CT or MRI were normal. CONCLUSION: The pure tone audiogram could preserve unchanged at early stage of auditory neuropathy, but their stapedial reflex and auditory brainstem response are absent or threshold elevated. The precise pathologic site of this disorder is yet to be defined, but it may locate in the auditory system below brainstem.

Adolescent↗

PubMed2002

[Sudden deafness with large vestibular aqueduct (case report and literatures review)].

OBJECTIVE: To study the clinical feature of large vestibular aqueduct syndrome and diagnostic basis of large vestibular aqueduct on high resolution computer tomography. METHOD: Report a 18 year old man who presented a sudden deafness in right ear and his left ear arised the same disorder before 11 months. Since childhood his both ears have occurred sensorineural hearing loss, which causations were reserved unknown. The temporal bone axially oriented high resolution computer tomography scanning showed a enlarged vestibular aqueduct without associated cochlear anomalies of both ears. In addition the literatures about this disorder are reviewed. RESULT: The large vestibular aqueduct syndrome without association cochlear anomalies may occurs acquired sensorineural hearing loss and appears to be at high risk for sudden deafness. CONCLUSION: It is important to identify followed by high-resolution computer tomography whether a large vestibular aqueduct syndrome when a causal uncertain sensorineural hearing loss or/and a sudden deafness onset in childhood is represented.

Adolescent↗