Biomedical subjects

Xiang-liang Yang

Publications and source records attributed to Xiang-liang Yang.

7 recordsLinked to original sources

PubMed2006

[Methodological study on magnetic enzymic immunoassay for detecting free hCGbeta subunit].

AIM: To establish a novel magnetic enzyme immunoassay (MEIA) for detecting human choriogonadotropin free beta subunit (hCGbeta). METHODS: Two monoclonal antibodies (mAb) were used for conjugating with FITC and with alkaline phosphatase(AP) respectively, which incorporated magnetic solid phase separation. Magnetic beads were coupled with sheep anti-FITC antibody as solid phase, and phenolphthalein monophosphate was used as substrate to set up MEIA for detecting hCGbeta. RESULTS: The sensitivity of hCGbeta MEIA kit reached 0.1 IU/L. The intraassay variation and inter-assay variation was 8.5% and 14% respectively, with the average recovery rate of dilution of 92.5%. The kit showed no cross reactivity to LH, TSH and FSH, while a cross reactivity of 1.2% to intact hCG at concentration of 1000 IU/L. The time of efficacy of hCGbeta MEIA kit was longer than 14 months. CONCLUSION: hCGbeta MEIA kit is better than hCGbeta radio immunoassay and ELISA kit, which can provide a high-qualitative and cheap hCGbeta kit for market.

Antibodies, Monoclonal↗

PubMed2006

[Design and manufacture of microneedles array for transdermal drug delivery].

It is very important to disrupt the stratum corneum structure and to create pathways allowing transport of macromolecules, as the traditional transdermal drug delivery has been severely limited by the skin barrier. With the development of the Micro Electro-Mechanical System (MEMS), it becomes possible for microneedles array to strengthen the transdermal drug delivery. In addition to the increase of the skin permeability, it can also be used to deliver drugs into skin, such as insulin and vaccine, providing a new direction for drug delivery systems. In this paper, we review the development and applications in transdermal drug delivery of microneedles' array. The commercial prospects and recommendations for the future research work are also represented.

Administration, Cutaneous↗

PubMed2005

[Progress in research on triptolide].

To further understand triptolide, this paper has introduced the pharmacology, pharmacokinetics, toxicity, the clinic application and semi-synthesis of triptolide on basis of importance and significant contents of reference which have been consulted in the past twenty years. Presently triptolide and Tripterygium wilfordii have been a hot spot of modernization of Chinese traditional medicine. It is very important to develop a new dosage form of high effect and low toxicity by making use of advanced technology according to its characteristics.

Animals↗

PubMed2005

[Enhanced absorption of breviscapine photosomes in small intestine of rats].

OBJECTIVE: To investigate whether the absorption of breviscapine can be enhanced by using breviscapine photosomes. METHOD: Testing the uptake and intestinal permeability of breviscapine powder and breviscapine photosomes by using intestine perfusion technique and reverted gut sac method. RESULT: The uptake and permeability coefficient of breviscapine were increased in photosomes. The absorption process obeyed the Fick's law in the range of 0-100 microg x mL(-1). CONCLUSION: The absorption breviscapine photosomes is enhanced by increasing the permeability through a passive mechanism.

Animals↗

PubMed2005

[Isopropyl myristate molecular gels and drug-loaded transdermal capability].

AIM: To prepare of isopropyl myristate (IPM) molecular gels and investigate of its transdermal capability. METHODS: Microstructure of IPM gels was studied by scanning electron microscope (SEM) and optical microscope (OM). The rheology and thixotropy of IPM gels were investigated by viscosity. Triptolide was used as model drug to investigate its transdermal capability. RESULTS: The microstructure of IPM gels was a three-dimension network formed by the aggregation of Span 60 in IPM, which was rod-like tubular aggregate. It has good rheology and thixotropy. There was a good linear correlation between the accumulative permeated amount per unit area and the time for triptolide-loaded IPM gels. The permeation process agreed with zero order pharmacokinetics. The average permeability through rat skin for triptolide was 19.26 ng x cm(-2) x h(-1), which was 2.92 times of triptolide unguents obtained commercially available. CONCLUSION: Isopropyl myristate molercular gel can be formed by span 60 assemblies. Transdermal capability drug-loaded IPM gels was better than that of triptolide unguents.

Administration, Cutaneous↗

PubMed2004

Supercritical fluid extraction of sapogenins from tubers of Smilax china.

Supercritical CO(2) fluid extraction (SFE-CO(2)) was used to extract the sapogenins after acid hydrolysis from Smilax china tubers. The influence of extraction variables, such as modifier, pressure, temperature and extraction time, were studied. SFE-CO(2) was found to produce higher yield than conventional solvent extraction. The highest yield (0.454%) of sapogenins, mostly containing diosgenin, was obtained using 35 MPa pressure, 65 degrees C and 95% EtOH as a modifier for 180 min, higher than that obtained with conventional extraction methods (0.385%).

Chromatography, Supercritical Fluid↗

PubMed2004

[Characterization of Me. PEG-PLA copolymer nanoparticles prepared by modified spontaneous emulsion-solvent evaporation].

AIM: Characterization of poly (D, L-lactic acid)/monomethylether terminated/polyethylene glycol (Me. PEG-PLA) block copolymers nanoparticles. METHODS: Me. PEG-PLA block copolymers were prepared by bulk polymerization. A series of nanoparticles were made from Me. PEG-PLA block copolymer by modified spontaneous emulsion-solvent evaporation technique. RESULTS AND CONCLUSION: The structure of copolymer was performed by means of 1HNMR and FT-IR. The morphological examination of nanoparticles was performed by means of atomic force microscope (AFM). Results indicated that nanoparticles exhibited a smooth spherulite and core-shell structure. The hydrophilic shell is consisted of PEG segments and hydrophobic core is consisted of PLA segments. Zeta potential of nanoparticles was zero and further indicated core-shell structure. The particle size and size distribution of nanoparticles were measured by laser light scattering technique. The effective particle size range was from 70 to 160 nm and showed a normal distribution.

Drug Carriers↗