Biomedical subjects

Xiao-Ying Wu

Publications and source records attributed to Xiao-Ying Wu.

7 recordsLinked to original sources

PubMed2005

[Operation performance of membrane nitrification bioreactor for ammonia-containing wastewater].

The operation performance of membrane nitrification bioreactor to treat ammonia-containing wastewater as well as the capabilities of separation and filtration of polypropylene membrane modules were tested. The removal efficiency of ammonia was kept higher than 95% when the hydraulic retention time was set at 1 day, the influent concentration was increased up to 80 mmol (NH4+ -N) x L(-1) and the volume loading rate was increased up to 1.12 kg (NH4+ -N) x m(-3) x d(-1). The biomass in the reactor was accumulated from 5 g x L(-1) to 10 g x L(-1) within 50 days, which indicated that polypropylene membrane modules were efficient in retaining biomass. The biomass attached to the membrane also contributed to the conversion of ammonia and nitrite. When the hydraulic pressure was lower than 80cm, the increase of hydraulic pressure improved the permeation of membrane. However, when the hydraulic pressure was beyond 80cm, the increase of hydraulic pressure did not significantly improve the permeation of membrane. The permeate flux was the highest 2.51 (L x m (-2) x h(-1)) but the resistance was the lowest (2.63 x 10(-5)) m(-1) when the hydraulic pressure was about 20 cm. The results showed that the membrane nitrification bioreactor could be run normally without extra energy input.

Biodegradation, Environmental↗

PubMed2005

[Multiple factor analysis of visual function in hypermetropic children].

OBJECTIVE: To investigate the characteristics of hypermetropic children whose visual acuity (VA) is declined or accompanied by esotropia. METHODS: One hundred and ninety children were given optometry, strabismus degree and binocular vision measurement. RESULTS: Declined VA appeared in 170 children, while esotropia occurred in 173. Sixty-one got binocular single vision, 17 had fusion function, and 11 had stereoaculty. Spherical equivalent had a linear relationship with VA (P < 0.01), but not with the strabismus degree (P > 0.05). The influence factors of binocular vision were age of discovery, VA and the strabismus degree, while the stereoaculty was influenced by the strabismus degree, spherical equivalent and VA. CONCLUSION: Low VA and strabismus are the most common complaint in children. Ametropia and strabismus do great harm to juvenile binocular vision, and stereoaculty is damaged seriously. We suggest an early examination of visual function in children.

Adolescent↗

PubMed2004

[Differential analysis of two-dimension gel electrophoresis profiles of human lung squamous carcinoma and tumor-adjacent tissue].

BACKGROUND & OBJECTIVE: Carcinogenesis of lung squamous carcinoma is a complex process involving multiple events and steps. Though some molecular pathogenesis studies on human lung cancer have been undertaken successfully in gene (DNA) and transcription (mRNA) levels, the carcinogenic mechanism is still unclear. At present, there is no special molecular marker for early-stage diagnosis and prognosis evaluation. The objective of this study was to establish two-dimensional electrophoresis profiles with high resolution and reproducibility from human lung squamous carcinoma tissue and paired normal tumor-adjacent bronchial epithelial tissue, and to identify differential expression proteins. METHODS: The total proteins of human lung squamous carcinoma tissue and paired normal tumor-adjacent bronchial epithelial tissue were separated by immobilized pH gradient (IPG)-based two-dimensional gel electrophoresis (2-DE). The differential expression proteins were analyzed using image analysis software, then identified using mass spectrometry and database searching. RESULTS: (1) For tumor tissues, the average protein spots of 3 gels were 1349+/-67; and 1235+/-48 spots were matched with the average matching rate of 91.5%. For control, the average protein spots of 3 gels were 1297+/-73; and 1183+/-56 spots were matched with the average matching rate of 91.2%. The average position deviation of matched spots was 0.873+/-0.125 mm in IEF direction, and 1.025+/-0.213 mm in SDS-PAGE direction. (2) A total of 1069+/-45 spots were matched between the electrophoretic maps of 15 human lung squamous carcinoma tissue and paired normal tumor-adjacent bronchial epithelial tissue. Forty differential proteins were identified by peptide mass fingerprint(PMF), some proteins were the products of oncogenes, and the others involve in the regulation of cell cycle and signal transduction. CONCLUSION: In this study, the well-resolved, reproducible 2-DE patterns of human lung squamous carcinoma and adjacent normal bronchial epithelial tissues were established and certain differential proteins were characterized. These data will be helpful for screening the biomarker to further study on human lung squamous carcinoma.

Amino Acid Sequence↗

PubMed2004

[2-DE profiling and differential analysis of human bronchial epithelial tissues in different stages of carcinogenesis].

BACKGROUND & OBJECTIVE: The carcinogenesis of bronchial epithelial cells is a complex multiple-stage process involving multiple genes, but its mechanism remains unclear. Studying this process with proteomic approaches may identify carcinogenesis-associated proteins, which are important for elucidating carcinogenic mechanism of human lung squamous carcinoma. This study was designed to optimize the protein preparation methods for bronchial epithelial tissues, to establish two-dimensional gel electrophoresis profiles of human bronchial epithelial tissues from different stages in carcinogenic process, and to perform differential analysis and provide a basis for identifying carcinogenesis-associated proteins of lung squamous carcinoma. METHODS: After obtaining samples of the normal, metaplasia, dysplasia, carcinoma tissues of human bronchial epithelia, modified deoxycholate- trichloroaetic acid (DOC-TCA) precipitation was used to extract and purify the total proteins of bronchial epithelial samples. Immobilized pH gradient two-dimensional polyacrylamide gel electrophoresis (2-DE) was used to separate the total proteins of the samples. After silver staining, ImageMaster 2-DE image analysis software was applied to analyze 2-DE images. Some selected differential protein spots were identified by peptide mass fingerprint (PMF) based on matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and database search. RESULTS: The total proteins extracted with the method described here were used to perform 2-DE. 2-DE patterns with high resolution and reproducibility from different stages were obtained. The average spots for normal epithelium, metaplasia, dysplasia and invasive carcinoma were 1189.50+/-39.89, 1227.00+/-37.90, 1273.00+/-43.31, and 1326.00+/-66.63, respectively. The test was repeated, which showed that there were average 1216 +/- 75 spots among 3 gels of the same metaplasia tissues and 1082 +/- 67 spots were matched. The average matching rate was 89.3% and protein spots in 3 gels had good reproducibility. The average position deviation of matched spots in different gels was 0.865+/- 0.247 mm in IEF direction, and 0.971+/- 0.104 mm in SDS-PAGE direction. The 2D images of 32 samples were compared, which showed a significant difference of the number of average protein spots among the four groups (P< 0.05). The average differential spots between the low and advanced stages were 31.50 +/- 7.67, 41.00 +/- 9.07, and 56.00 +/- 8.96, respectively. Twenty-three protein spots chosen randomly from dysplasia and invasive carcinoma groups were identified by PMF, some of which were involved in the cell proliferation, differentiation, cycle regulation, signal transduction and tumor occurrence. CONCLUSIONS: (1) Improved DOC-TCA precipitation is a preferable method for protein preparation from bronchial epithelial tissues. (2) We established well-resolved, reproducible 2-DE profiles of human bronchial epithelial tissue in different stages of carcinogenesis. Some differentially expressed-proteins may be related to carcinogenesis of bronchial epithelia. These results provide a fundamental basis for further study of carcinogenic mechanism of lung squamous cancer and screening its specific marker.

Bronchi↗

PubMed2004

[Establishment of protein profile of human small cell lung cancer cell line NCI-H446].

BACKGROUND & OBJECTIVE: Small cell lung cancer (SCLC) is particularly aggressive, and characterized by rapid growth and early metastasis. At present, there is no data concerning SCLC two-dimensional polyacrylamide gel electrophoresis (2-DE) reference map,and its protein profiles in public databases. This study was to establish a well-resolved, reproducible 2-DE map of proteome in SCLC cell line NCI-H446, and analyze its protein profiles. METHODS: Two-DE was applied to separate the total proteins of NCI-H446 cells, which were then silver-stained in the gel. Well-separated protein spots were selected from the gel by ImageMaster 2D analysis system. Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS), peptide map fingerprinting (PMF),and database searching were used to identify the protein spots. RESULTS: Clear,well-resolved, reproducible 2-DE patterns of proteome in NCI-H446 cells were obtained. The average protein spots of 3 gels were 1506+/-74; and 1412+/-56 spots were matched with an average matching rate of 93.4%. The average deviation of spot position was (0.96+/-0.27) mm in IEF direction, and (1.24+/-0.41) mm in SDS-PAGE direction indicating relatively good reproducibility of the protein spots. Fifty-eight proteins were identified, certain proteins were products of oncogenes, and others were involved in cell cycle regulation, and signal transduction. CONCLUSIONS: A reference map of NCI-H446 cells was established,certain proteins were identified by MALDI-TOF-MS and PMF. These data will be useful for establishing human SCLC proteome database.

Amino Acid Sequence↗

PubMed2004

[Improvement of protein preparation methods for bronchial epithelial tissues and establishment of 2-DE profiles from carcinogenic process of human bronchial epithelial tissues].

OBJECTIVE: To optimize the protein preparation methods for bronchial epithelial tissues, to establish two-dimensional gel electrophoresis profiles from different stages tissues of human bronchial epithelial carcinogenic process, and to provide a base for identifying carcinogenesis-associated proteins of lung squamous carcinoma. METHODS: After obtaining samples of the normal-metaplasia-dysplasia-carcinoma tissues of human bronchial epithelia, improved deoxycholate-trichloroaetic acid (DOC-TCA) precipitation was used to extract and purify the total proteins of bronchial epithelial samples. Immobilized pH gradient two-dimensional polyacrylamide gel electrophoresis (2-DE) was used to seperate the total proteins of the samples. After silver staining, ImageMaster 2-DE image analysis software was applied to analyze 2-DE images. RESULTS: The total proteins extracted with improved DOC-TCA precipitation was used to perform 2-DE. 2-DE patterns with high resolution and reproducibility from different stages were obtained. The average spots for normal epithelium, metaplasia, dysplasia and invasive carcinoma were 1190 +/- 63, 1 227 +/- 69, 1272 +/- 71 and 1326 +/- 82, respectively. One of the squmous metaplasia tissues was chosen and repeated 2-DE for 3 times. Averagely 1216 +/- 75 spots were detected among the 3 gels of metsplasia tissues and 1082 +/- 67 spots were matched. The average matching rate was 89.3% and protein spots in the 3 gels had a good reproducibility. The average position deviation of matched spots in different gels was 0.835 +/- 0.247 mm in IEF direction, and 0.921 +/- 0.104 mm in SDS-PAGE direction. CONCLUSION: Improved DOC-TCA precipitation is a proper method for protein sample preparation of bronchial epithelial tissues. The well-resolved, reproducible 2-DE profiles from different stage tissues of human bronchial epithelial carcinogenic process have been primarily established.

Bronchi↗

PubMed2003

Study on relationship between expression level and molecular conformations of gene drugs targeting to hepatoma cells in vitro.

AIM: To increase exogenous gene expression level by modulating molecular conformations of targeting gene drugs. METHODS: The full length cDNAs of both P(40) and P(35) subunits of human interleukin 12 were amplified through polymerase chain reaction (PCR) and cloned into eukaryotic expressing vectors pcDNA3.1(+/-) to construct plasmids of P(+)/IL-12, P(+)/P(40) and P(-)/P(35). These plasmids were combined with ASOR-PLL to form two targeting gene drugs [ASOR-PLL-P(+)/IL-12 and ASOR-PLL-P(+)/P(40) + ASOR-PLL-P(-)/P(35)] in optimal ratios. The conformations of these two drugs at various concentrations adjuvant were examined under electron microscope (EM) and the drugs were transfected into HepG2 (ASGr+) cells. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) was performed with total RNA extracted from the transfected cells to determine the hIL12 mRNA transcript level. The hIL12 protein in the cultured supernatant was measured with enzyme-linked immunosorbent assay (ELISA) 48 hours after transfection. RESULTS: Targeting gene drugs, whose structures were granular and circle-like and diameters ranged from 25 nm to 150 nm, had the highest hIL-12 expression level. The hIL-12 expression level in the group co-transfected with ASOR-PLL-P(+)/P(40) and ASOR-PLL-P(-)/P(35) was higher than that of ASOR-PLL-P(+)/IL-12 transfected group. CONCLUSION: The molecular conformations of targeting gene drugs play an important role in exogenous gene expression level, the best structures are granular and circle-like and their diameters range from 25 nm to 150 nm. The sizes and linking styles of exogenous genes also have some effects on their expression level.

Carcinoma, Hepatocellular↗