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Xiao-ping Liu

Publications and source records attributed to Xiao-ping Liu.

13 recordsLinked to original sources
PubMed2006

[Identification on Spora Lygodii by X-ray diffraction fingerprint spectra method].

OBJECTIVE: To set up a new identification and analysis method of Spora Lygodii. METHOD: To get the extracts of Spora Lygodii using methanol, chloroform and petroleum ether as solvent and the extracts were identified, analyzed by X-ray diffraction Fourier fingerprint spectra. RESULTS: Experiments and analysis were carried out on three samples. The standard X-ray diffraction Fourier fingerprint spectra and characteristic diffraction peaks were obtained. There were some differences among the spectra of the extracts, but the characteristic diffraction peaks were obvious. CONCLUSION: The experimental result indicated that X-ray diffraction Fourier fingerprint spectra can be used to identify and analyze the Chinese traditional herb Lygodium japonicum (Thunb.) Sw.

Ferns↗
PubMed2006

[Detection of MSH2 gene mutation by PCR].

OBJECTIVE: To establish a genetic diagnosis method for a novel MSH2 mutation. METHODS: A specific primer on the mutated site of MSH2 was synthesized and PCR was conducted using the specific primer and another downstream primer. PCR products were electrophoresed and then the carriers with the novel gene mutation of the carriers or non-carriers were identified. RESULTS: MSH2 in a hereditary nonpolyposis colorectal cancer family were successfully found. CONCLUSION: The method is effective and simple for genetic diagnosis of the novel mutation in MSH2.

Base Sequence↗
PubMed2005

[Surgery for iatrogenic obstruction of the second hepatic portal].

OBJECTIVE: To summarize the effects of surgery for iatrogenic obstruction of the second hepatic portal and the curative effects thereof. METHODS: Seven patients, 5 males and 2 females, aged 25-38, with iatrogenic obstruction of the second hepatic portal, due to blocking of the reflux of hepatic vein by translocated stent in 4 cases and due to occlusion of hepatic vein local thrombosis and endometrial hyperplasia caused by broken wires in 3 cases, underwent removal of the foreign bodies and plastic operation of the second hepatic portal under temporary circulatory pause and hypothermic general anesthesia via the approach of incision of the pericardium and inferior vena cava. The patients were followed up for 6 months to 5 years. RESULTS: After the operation the liver shrank and ascites was decreased significantly in all patients. The liver functions of all patients were normal during the follow-up and no complication or signs of recurrence were found. Ultrasonography and CT scan showed no stricture and stenosis. CONCLUSION: Translocation of stent is the common reason of iatrogenic obstruction of the second hepatic portal. Operational procedure including removal of the foreign bodies and plastic operation of the second hepatic portal under temporary circulatory pause and hypothermic general anesthesia via the approach of incision of the pericardium and inferior vena cava is an effective radical surgical treatment for this complication.

Adult↗
PubMed2005

[The endovascular repair of ruptured aortic aneurysm].

OBJECTIVE: To discuss the safety, feasibility, efficacy and problem of endovascular repair (EVR) for ruptured aortic aneurysm (RAA). METHODS: Fourteen patients with RAA were obtained diagnosis and anatomic data of proximal and distal neck through magnetic resonance imaging and CT. The patients with hypotension were accepted anti-shock treatment. Stent-grafts were deployed in proper position of RAA under X-ray fluoroscopic. RESULTS: The time from rupture to operation was 50 min to 21 d. Three cases had plenty of blood in left pleural cavity and 2 cases were done under bleeding shock condition. Five cases with RAA were fixed by bifurcated stent-grafts and others by straight stent-grafts. Follow-up term was 1 month to 38 months. One with ruptured thoracic aortic aneurysm died 4 h later after operation, another one died of left thoracic cavity infection 9 months later, and others lived well without complication. CONCLUSION: The EVR is a safe, efficient and feasible method. It will be a potential alternative to treat RAA in future, but more suitable for RAA with better proximal and distal landing zones.

Adult↗
PubMed2005

[The endovascular repair of aortic dissection: early clinical results of 178 cases].

OBJECTIVE: To discuss the safety, feasibility, efficacy and problems of endovascular repair for aortic dissection. METHODS: From June, 1998 to Dec, 2004, 178 aortic dissections were treated by stent-grafts, including 76 acute cases and 102 chronic cases, 19 cases with Stanford A and 159 cases with Stanford B. Under local or general anesthesia, every stent-graft was deployed at the proper position of first tear entry through femoral artery under X-ray fluoroscopic. The changes of hemodynamic in true and false lumen, visceral and limbs blood supply were investigated after operation. RESULTS: 10 cases combined with left common carotid artery or left subclavian artery or hepatic artery and superior mesenteric artery bypass. 36 left subclavian arteries were covered simultaneously without bypass and the average blood pressure of left brachial artery was (61.6 +/- 23.7) mm Hg. The stent-grafts were deployed above thoracic 8 in 159 cases and below thoracic 8 in 19 cases. This group included 3.4% 30-day death rate, 12.9% endoleak rate after deployment, and without misplace of stent grafts, migration, rupture, conversion to open surgery and paraplegia complication. The average operation time 1.5 h (0.5-4.3 h), blood loss 140 ml (30-500 ml), movement recover time 1.8 d (0.5-21.0 d), food recover time 1.5 d (0.5-9.0 d). The true lumen blood supply in most of damaged visceral arteries were improved. Follow up between 1 month to 76 months, the endoleak rate was 6.4% one month later. CONCLUSION: The endovascular repair is a safe, efficacy and feasible method to aortic dissection. The long term results keep in follow up.

Adult↗
PubMed2005

[Adenovirus containing c-FLIPs gene induces antiapoptosis activation of lymphocyte in vitro].

OBJECTIVE: To investigate the efficacy and mechanisms of recombinant adenovirus containing c-FLIPs (cellular FLICE-inhibitory protein short) in inducing antiapoptosis activation of lymphocyte in vitro. METHODS: c-FLIPs gene was cloned from total RNA of lymphocyte with RT-PCR methods, then constructed recombined adenovirus Ad.c-FLIPs by pAdeasy system. H9 cells infected with Ad.c-FLIPs produced antiapoptosis derived from anti-Apo-1 antibody, and those antiapoptosis evaluated with PI stain flow cytometry (FCM) and Hoechst stain. RESULTS: c-FLIPs gene was successfully cloned from lymphocytes and incorporated into recombinant adenovirus Ad.c-FLIPs.After exposure to anti-Apo-1 for 24 h, the apoptosis of H9 cells preconditioned by Ad.c-FLIPs for 24 h as measured by Hochst stain or FCM significantly decreased, compared with non-preconditioned cells, 3.60% +/- 0.21% vs. 48.33% +/- 7.41%, respectively, indicating that Ad.c-FLIPs preconditioning protected H9 cells against apoptosis induced by anti-Apo-1. CONCLUSION: Recombinant adenovirus Ad.c-FLIPs effectively induced anti-apoptosis activation of lymphocyte.

Adenoviridae↗
PubMed2005

[Study on classification and treatment of vulvovaginal candidiasis].

OBJECTIVE: To determine the clinical manifestations of vulvovaginal candidiasis (VVC) and to study the mycologic eradication rate of different miconazole treatment courses for VVC. METHODS: Three hundred cases of VVC were recruited. The Candidas were cultured. A prospective and randomized study was performed to compare the treatment effect of 3 day miconazole (400 mg/d), 6 day miconazole (400 mg/d), and 7 day miconazole (200 mg/d) for uncomplicated and complicated VVC. RESULTS: Among 300 cases of VVC, uncomplicated, complicated and recurrent VVC were 56.0%, 44.0% and 9.7% (29/300) respectively. C. albicans was isolated most frequently 90.3% (271/300), followed by C. glabrata (7.3%), C. tropicalis (1.3%), C. krusei (0.7%), and C. parapsilosis (0.3 %). Mycologic eradication rate of 3 day, 6 day and 7 day miconazole courses for uncomplicated VVC at day 14 was 96.0%, 93.5% and 98.0%, respectively (P > 0.05). Eradication rate of 3 day, 6 day and 7 day miconazole courses for complicated VVC at day 14 was 86.7%, 92.5%, and 86.4%, respectively (P > 0.05). Eradication rate of 3 day, 6 day and 7 day miconazole courses for uncomplicated VVC at day 35 was 93.8%, 95.3%, and 89.8%, respectively (P > 0.05). Eradication rate of 3 day, 6 day and 7 day miconazole courses for complicated VVC at day 35 was 89.7%, 97.3% and 86.8%, respectively (P > 0.05). CONCLUSION: Treatment of VVC should be individualized, and women with complicated VVC achieve superior mycologic eradication by a 6 day miconazole course.

Adult↗
PubMed2004

[A mutation IVS2+1G>A in EXT2 gene causes hereditary multiple exostoses].

OBJECTIVE: To identify the gene causing hereditary multiple exostoses in a Chinese pedigree. METHODS: Linkage analysis was carried out in the family using microsatellite markers on chromosome 8, 11 and 19 respectively. To detect the mutation, the whole coding sequence and the intron-exon boundaries of the candidate gene were amplified and sequenced. The reverse transcriptase-polymerase chain reaction (RT-PCR) was performed to amplify the mutated mRNA. RESULTS: The disease-causing gene of the family was linked to the EXT2 locus on chromosome 11. A mutation IVS2+1G>A was detected in EXT2 and resulting in 221 bp deletion from 316 to 536 of coding sequence(CDS), which was co-segregated with the disease phenotype. This change led to deletion from codon 106 to codon 178 and subsequent 2 nucleotides, producing a frameshift and truncated protein of 125 aa. CONCLUSION: The mutation IVS2+1G>A is the disease-causing mutation in the Chinese pedigree with hereditary multiple exostoses.

Exostoses, Multiple Hereditary↗
PubMed2003

[Prevention and management of frequent complications after endovascular repair of infrarenal abdominal aortic aneurysm].

OBJECTIVE: To prevent and manage frequent complications after endovascular repair of infrarenal abdominal aortic aneurysm (AAA). METHODS: The data of 71 cases of infrarenal abdominal aortic aneurysm (AAA) treated by endovascular repair were analysed retrospectively. The reasons, managements, results and prognosis of frequent complications were investigated. RESULTS: Seventy-one cases of infrarenal AAA were treated by endovascular repair with 100% success rate. There was no surgical conversion to open aneurysm repair. There were 8 cases of primary endoleak, 1 case of nervous complication and acute thrombosis. An average follow-up period was 26 +/- 5 months. Three persistent endoleaks and 4 secondary endoleaks were found during the follow-up period. The endoleak rate was 9.8% (7/71) within 1 month postoperatively and mortality rate was 1.3% (1/71). Total mortality rate was 4.2% (3/71). Two patients died from acute myocardial infarction and one from acute heart failure. CONCLUSIONS: Endovascular treatment of abdominal aortic aneurysm is technically feasible and can effectively exclude aortic aneurysms from the circulation. Endoleak is a chief complication after endovascular repair of infrarenal AAA.Additional procedures and follow up are very important. Endoleak with enlarged aneurysm should be treated actively.

Aged↗
PubMed2003

[The change of blood supply pattern in visceral arteries of Stanford B dissection after endovascular repair].

OBJECTIVE: To discuss the change of blood supply pattern in visceral arteries of Stanford B dissection. The visceral arteries include celiac trunk (CA), superior mesenteric artery (SMA) and renal artery (RA). METHODS: By retrospectively analysing the clinical data of 52 cases with Stanford B dissection, the blood supply pattern of visceral arteries was confirmed by aortography and the changes before and after endovascular repair were compared. RESULTS: After repair: the stenosis lesions disappeared in 7 cases supported by true channel completely but one. Twenty-two visceral arteries supported by true and false channel simultaneously recovered true channel chiefly but one. One recovered true channel chiefly and one had no change in 2 visceral arteries supported by false channel completely. Four recovered true channel chiefly and one had no change in 5 visceral arteries without blood support. 88.9% blood support got better and 11.1% blood support had no change in 36 damaged visceral arteries after endovascular repair. CONCLUSION: Blood support from true and false channel simultaneously is the chief pattern in the injured visceral arteries before repair; Endovascular repair technique is benefit to recovering the blood support of true channel.

Adult↗
PubMed2002

[Expression of growth hormone receptor mRNA in hepatocellular carcinoma and matched para-cancer cirrhotic liver tissue].

BACKGROUND & OBJECTIVE: The growth hormone receptor mRNA is abundant in normal liver tissue, but its expression was not studied in detail in the tissue of liver cancer or para-cancer cirrhotic liver. This study was designed to investigate the expression of growth hormone receptor mRNA in hepatocellular carcinoma and para-cancer cirrhotic liver tissue. METHODS: The expression of growth hormone receptor mRNA was detected in 37 specimens of hepatocellular carcinoma and their para-cancer liver tissue by RT-PCR. RESULTS: The expression rate of growth hormone receptor mRNA in hepatocellular cancerous tissue (30/37, 81%) was significantly lower than that in cirrhotic liver tissue (32/32, 100%) (P < 0.05). The expression rate in undifferentiated hepatocellular carcinoma (1/4, 20%) decreased further (P < 0.05). The expression of growth hormone receptor mRNA in cirrhotic liver tissue [ratio of integrated Optical Density (riOD) 30.77% +/- 8.24%, n = 32] was significantly lower than that in normal control liver tissue (riOD 44.93% +/- 6.25%, n = 5) (P < 0.05). The expression in severe cirrhotic liver tissue (riOD 21.90% +/- 4.72%, n = 8) decreased further (P < 0.05). CONCLUSIONS: The down-regulated expression of growth hormone receptor mRNA was related to the pathological stage of hepatocellular cancer and the stage of liver cirrhosis.

Adult↗