Biomedical subjects

Xiao-xia Wang

Publications and source records attributed to Xiao-xia Wang.

7 recordsLinked to original sources

PubMed2006

[Expression of Crg-2 by a bacteria recombinant system of adenovirus].

AIM: To express an immunochemotactic and angiostatic anti-tumor molecule, CXC chemokine Crg-2, by E. coli recombinant system of adenovirus. METHODS: By E. coli recombination system of adenovirus, the shuttle vector pShuttle-cmv/crg-2 was co-transfected into BJ5183 E. coli with adenovirus backbone plasmid pAdEasy-1. The recombinants were transfected into 293 packaging cells and adenovirus was packaged and amplified. The molecular weight and bioactivity of recombinant protein were determined by Western blot and chemotaxis assay, respectively. RESULTS: Recombinant adenovirus genome backbone bearing crg-2 gene, pAd/crg-2, was successfully obtained as evidenced by endonucleases digestion and PCR. Adenovirus was packaged and amplified to a titre of 4x10(9) TCID50/L. The cultured supernatant of the infected 293 cells contented a protein of nearly 10,000 and presented a significantly chemotatic effect towards activated spleen lymphoblasts. CONCLUSION: Recombinant adenovirus Ad/crg-2 obtained by E. coli recombinant system of adenovirus can efficiently express bioactive Crg-2 chemokine.

Adenoviridae↗

PubMed2005

[Effects of maxillary distraction osteogenesis on the velopharyngeal configuration of cleft palate patients].

OBJECTIVE: To study the effects of internal maxillary distraction osteogenesis(DO) on the velopharyngeal configuration of cleft palate patients. METHODS: Ten patients with severe maxillary hypoplasia secondary to cleft lip and palate patients (7 males and 3 females, average age 20.1 years old) had undertaken high step LeFort I osteotomy, and internal maxillary distraction devices were applied to advance the maxilla. Before surgery, when DO was completed and 6 months after DO was completed, oriented lateral cephalograms at rest position of each patient were taken, and 6 measure indexes of velopharyngeal configuration were collected and analyzed. RESULTS: All patients had successfully accomplished maxillary DO and the maxilla had been averagely advanced 11.3 mm. PNS-PhW, C-PhW, UL and ANS-PNS-T had all significantly increased, and UD had significantly decreased when DO was completed and 6 months after DO was completed as compared with pre-surgery. No significant linear correlation was found between maxilla advancement distance and velopharyngeal configuration changes. CONCLUSION: Correction of maxillary hypoplasia secondary to cleft palate surgery by using internal maxillary DO can increase the velopharyngeal cavity depth, and may impair velopharyngeal competence, but the compensatory changes of velopharyngeal soft tissue can alleviate this impairment to certain extent.

Adolescent↗

PubMed2005

[Development of a prototype of space vegetable-cultivating facility for ground-based experiments].

OBJECTIVE: To develop a ground-based experimental prototype of space vegetable-cultivating facility (GEPSVF), so as to solve the main key techniques related to higher plant cultivation in space environmental conditions, and to further lay a foundation for future development and application of the prototype of space vegetable-growing facility. METHOD: Based on detailed demonstration and design of technique plan, the blueprint design and machining of components, whole facility installment, debugging, trial operations and verification experiments were done. RESULT: The parameters in the growing chamber such as temperature, relative humidity, wind velocity, total pressure, O2 partial pressure, CO2 partial pressure and water content of the growing media were totally and effectively controlled; the light source was electronic fluorescent lamp; the average vegetable-producing output reached 60 g (fresh weight) d-1. CONCLUSION: The prototype could operate stably; its actual performance indexes achieved the expected ones basically; some of its operating principles can adapt to space microgravity condition. Therefore, the prototype is a good beginning for future development of space vegetable-producing facilities.

Atmospheric Pressure↗

PubMed2005

[An experimental study of the effect of structure sealing of infrared CO2 sensor on its accuracy].

OBJECTIVE: To study the effect of structure sealing of infrared CO2 sensor on its accuracy. METHOD: Two experiments were designed. The IR CO2 sensor based on infrared principle and no comparing lighting path with only one beam of light were calibrated in one experiment, and in the other experiment the sensors were placed in an environment simulating the practical condition. The temperature, pressure and standard gas were kept same in the two experiments while CO2 concentrations in the dead volume were varied. RESULT: Readings from the sensors varied with the variation of the CO2 concentration in the dead volume of the sensors. CONCLUSION: The structure sealing of the IR CO2 sensor has great influence on its accuracy. A sealed dead volume in the IR CO2 sensor can decrease or eliminate the effect.

Atmospheric Pressure↗

PubMed2005

[The transformation of fibroblast-like cells originating from different tissue into osteoblasts].

OBJECTIVE: To discuss whether fibroblast-like cells can transform into osteoblasts under specific condition during distraction osteogenesis. METHODS: Fibroblast-like cells were cultured in vitro. BMP-2, TGF-beta(1), BMP-2 and TGF-beta(1) were added into the medium, and the cellular change were observed by histochemistry and immunohistochemistry methods. The distraction was given on the membrane of fibroblast-like cells, and the cellular change were observed under the distraction. RESULTS: fibroblast-like cells from the joint could transform into osteoblasts under the effect of BMP-2 or BMP-2 with TGF-beta(1). Fibroblast-like cells from other regions could not transform into osteoblasts. CONCLUSION: The osteoblasts during distraction osteogenesis are multi-origin.

Animals↗

PubMed2004

[The effect of platelet-rich plasma on the distraction osteogenesis].

OBJECTIVE: To observe the effect of the platelet-rich plasma (PRP) on osteogenesis during bone distraction. METHODS: The osteoblasts were cultured in vitro. The rabbits were used as the animal model of distraction osteogenesis. The proliferation of osteoblasts was analysed through MTT methods. The osteogenesis was observed by histochemical and histoimmunochemical methods. RESULTS: PRP stimulated the proliferation of osteoblasts and facilitated distraction osteogenesis. CONCLUSIONS: PRP could accelerate the osteogenesis during bone distraction. The application of PRP in clinical practice might shorten the period of distraction osteogenesis.

Animals↗

PubMed2004

[Development of a ground-based experimental facility for space waste material processing with microorganism].

OBJECTIVE: To develop a ground-based experimental facility for microorganism waste processing, which will be used to recover nutrient from plant inedible biomass essential for growth and development of plants. METHOD: After technical parameters and performance requirements were defined, planning demonstration, drawing design, fabrication, debug and plant inedible residue-biodegradation tests by microorganisms were conducted. RESULT: The facility worked well, and the parameters, such as energy consumption, volume and weight, met the design requirement. The water-treated quality and the ability of treating plant residual by microorganism were better than the demands. The ground-based results demonstrated that total organic carbon (TOC) degradation above 92.1%, and chemical oxygen demand (COD) reduction over 95.5% could be maintained. CONCLUSION: The facility has reasonable technical indexes, and smooth and reliable performances. Its major working principle is suitable for the demand of space conditions. It is capable of being utilized for biodegradation of plant inedible biomass in space.

Aspergillus niger↗