Biomedical subjects

Xing-Zhi Wang

Publications and source records attributed to Xing-Zhi Wang.

7 recordsLinked to original sources

PubMed2005

Quantitative transcript analysis in plants: improved first-strand cDNA synthesis.

The quantity and quality of first-strand cDNA directly influence the accuracy of transcriptional analysis and quantification. Using a plant-derived alpha-tubulin as a model system, the effect of oligo sequence and DTT on the quality and quantity of first-strand cDNA synthesis was assessed via a combination of semi-quantitative PCR and real-time PCR. The results indicated that anchored oligo dT significantly improved the quantity and quality of alpha-tubulin cDNA compared to the conventional oligo dT. Similarly, omitting DTT from the first-strand cDNA synthesis also enhanced the levels of transcript. This is the first time that a comparative analysis has been undertaken for a plant system and it shows conclusively that small changes to current protocols can have very significant impact on transcript analysis.

Blotting, Southern↗

PubMed2005

[The analysis of rbcS gene function by post-transcription gene silencing in Nicotiana benthamiana].

A system of virus-induced post-transcriptional gene silencing for studying rbcS gene function was established and optimized using tobacco rattle virus vector and Nicotiana benthamiana as experimental materiaes. The following analyses were conducted: phenotypic characterization of rbcS gene silenced plants, transcription levels of rbcS gene by RT-PCR; protein levels of rbcS by the antibodies of rbcS and rbcL and photosynthetic pigments wntents in rbcS silenced plants by HPLC method. The results showed that the seedlings at 21-24-day-old and Agrobacterium concentration at OD600 = 1-1.5 gave the best results for gene silencing. The expression level of rbcL was very likely regulated by rbcS, and rbcS gene did not relate to the collection of photosynthetic energy. Probability analysis showed that the tobacco rattle virus vector system is a useful and effective technique to study rbcS gene function via post-transcriptional gene silencing.

Agrobacterium tumefaciens↗

PubMed2004

Preparation of polyclonal antibodies of Rubisco large and small subunits and their application in the functional analysis of the genes.

Spinach Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) large (rbcL) and small (rbcS) subunits were separated by SDS-PAGE, and protein amount and purity were determined by Bradford assay. Polyclonal antibodies against rbcL and rbcS subunit were generated in female BALB/c mice and had no cross-reaction with each other. A total of 81 microg antigens were used and 0.3 ml anti-sera with titer of 1:5000 were yielded. The antibodies were also applicable to study rbcL and rbcS in tobacco plant Nicotiana benthamiana. Potato virus X vector pGR107 induced silencing of rbcS gene by Agrobacterium in Nicotiana benthamiana was performed. The expression level of rbcL and rbcS was lower in rbcS silenced plants than that in control plants as detected by the corresponding antibodies. This implied that the expression of rbcL was regulated by rbcS.

Animals↗

PubMed2003

Molecular cloning and characterization of betaine aldehyde dehydrogenase gene from Suaeda liaotungensis and its use in improved tolerance to salinity in transgenic tobacco.

cDNA encoding betaine aldehyde dehydrogenase (BADH) from the halophyte Suaeda liaotungensis has been cloned, sequenced and expressed in tobacco (Nictiana tabacum 89). The full-length cDNA is 1506 base pairs (bp) long and encodes a 502 amino-acid polypeptide. The cDNA fragment coding for the mature enzyme was cloned into vector pCAMBIA-1301 for expression in tobacco. Southern blotting analysis showed that that the Badh gene was integrated into the genome of tobacco. Tobacco expressing BADH survived on MS medium containing 200 mM NaCl, whereas the untransformed plants turned yellow after about 20 d and died.

Aldehyde Oxidoreductases↗

PubMed2003

[Plants as bioreactor for the production of pharmaceutical proteins].

Transgenic plant as bioreactor has been used to produce recombinant proteins for medicinal purposes, including mammalian antibodies, blood substitutes and vaccines. As the demand for biopharmaceuticals is expected to increase, transgenic plants have the potential to provide virtually unlimited quantities of proteins for use as tools in both human health care and the bioscience. This paper reviews the recent developments in this field and the prospect of commercial applications.

English Abstract↗

PubMed2002

[Research progress in salt tolerance genes of SOS in Arabidopsis thaliana].

The soil salination is a significant abiotic stress for agricultural production and ecological environment. The research on salt tolerance represents an important part for basic plant biology. Genetic analysis of salt tolerance genes in Arabidopsis has become a central issue in this research areas. In recent years, efforts from some laboratories in the world have led to some significant progresses in this field. In this paper, we will review the progress in salt tolerance genes SOS (salt overly sensitive): SOS1,SOS2 and SOS3.

English Abstract↗

PubMed2002

[Research progress of cellulose synthase genes in higher plant].

Cellulose is a major component in plant cell wall. About 180 billion tons of cellulose are produced per year in nature. The commercial importance of cellulose makes the genes coding it one of attractive targets for plant genetic engineering. A number of cellulose synthase genes have been first cloned from plant species by Delmer's group in 1996. Recently, research achievement has been obtained in accumulating to understanding the cellulose synthase function,location,and the gene function. The paper summarized the research progress of cellulose synthase genes in higher plants.

English Abstract↗