Xue-xin Gu | PubMed Health

PubMed2006

[Determination of scopolamine and atropine in Flos Daturae by RP-HPLC].

OBJECTIVE: To develop a quantitative analytical procedure of scopolamine and atropine in Flos Daturae using RP-HPLC. METHOD: The two alkaloids were separated on a Hypersil BDS C18 column (4.6 mm x 250 mm, 5 microm) with a mobile phase of 0.02 mol x L(-1) sodium acetate buffer (containing 0.02% triethanolamine and the pH was adjusted to 6.0 with acetic acid)-methanol (60:40) and a detection wavelength of 215 nm. The flow rate was 1.0 mL x min(-1) and the column temperature was maintained at room temperature. RESULT: The mean recovery was (99.6 +/- 1.8)% for scopolamine and (100.4 +/- 1.5)% for atropine. CONCLUSION: This method was simple, accurate and sensitive.

Atropine↗

PubMed2004

[Solid phase extraction and room temperature phosphorimetry coupling technique and its application].

The principle and application in the environmental analysis of Solid Phase Extraction (SPE) combined with Room Temperature Phosphorescence (RTP) analysis were reviewed. The principle, device, separation modes, operation procedures and application of SPE were commented. The merits and shortages in the operation procedure and quantitative analysis of classic RTP were also summarized. The potential application in the trace sample analysis and the advantage of coupling technique of SPE and RTP were indicated. The sensitivity and selectivity of the coupling technique could be greatly increased because of the strong enrichment ability of the SPE materials and the high selectivity of RTP. So far the technique has been successfully used in the analysis of polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and polychlorinated dibenzofurans (PClDBFs) in the environmental samples according to their phosphorescence emission after preconcentration on the Whatman 1PS filter paper and C18 or C8 SPE membranes.

Adsorption↗