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Biomedical subjects

Y C Hong

Publications and source records attributed to Y C Hong.

At least 19 recordsLinked to original sources
PubMed1998

Measurement of prostaglandin E2 and leukotriene B4 in the gingival crevicular fluid.

UNLABELLED: The arachidonic acid metabolites prostaglandin E2 (PGE2) and leukotriene B4 (LTB4) are inflammatory mediators which are likely to be involved in the pathogenesis of periodontal disease. PGE2 mediates vasodilatation, increases vascular permeability, enhances pain perception by bradykinin and histamine, alters connective tissue metabolism and enhances osteoclastic bone resorption. LTB4 causes the accumulation of inflammatory cells in the inflamed sites, and degranulation of polymorphonuclear leukocytes. OBJECTIVE: To measure gingival crevicular fluid (GCF) levels of PGE2, LTB4 and periodontal health. METHODS: The periodontal condition of 24 subjects was evaluated on the basis of plaque index, gingival index, probing depth, and attachment level. GCF samples were collected from one or two site(s) of each sextant per subject and the volume was measured using Periotron 6000. Samples were then assayed for PGE2 and LTB4 using a competitive enzyme immunoassay. Mean PGE2 and LTB4 levels were determined for each subject and group means compared. RESULTS: Significant differences in the levels of PGE2 and LTB4 were found between patients with periodontitis, and non-periodontitis individuals (P < 0.001). The PGE2/LTB4 levels were positively correlated with the clinical parameters (P < 0.01) and reduced markedly after phase 1 of the periodontal treatment (P < 0.01). The total amount and concentration (ng ml-1) of LTB4 was positively correlated with the gingival index (P < 0.01). CONCLUSIONS: These results indicate that the levels of PGE2 correlated with the severity of the periodontal status, and the levels of LTB4 correlated with gingival inflammation. Thus, our data suggest that the total amounts of PGE2/LTB4 may be good indicators for periodontal inflammation.

Adult↗
PubMed1997

Magnesium inhibits nickel-induced genotoxicity and formation of reactive oxygen.

Nickel compounds are recognized to cause nasal and lung cancers. Magnesium is an effective protector against nickel-induced carcinogenesis in vivo, although its mechanisms of protection remain elusive. The effects of magnesium carbonate on the cytotoxicity and genotoxicity induced by nickel subsulfide were examined with respect to the inhibition of cell proliferation, micronuclei formation, DNA-protein cross-link formation, and intranuclear nickel concentration. The generation of reactive oxygen by nickel chloride was also analyzed by observing 8-hydroxy-deoxyguanosine formation from deoxyguanosine in the presence and absence of magnesium chloride. The suppression of up to 64% of the proliferation of BALB/3T3 fibroblasts by nickel subsulfide (1 microgram/ml) was reversed by magnesium. The nickel compound increased not only the number of micronuclei but also the amount of DNA-protein cross-links examined with CHO and BALB/3T3 cells, respectively. These genotoxic effects of nickel were again lessened by magnesium carbonate. In addition, the cellular accumulation of nickel increased 80-fold with nickel subsulfide treatment and decreased with magnesium carbonate treatment. Nickel also enhanced 8-hydroxy-deoxyguanosine formation in the presence of H2O2 and ascorbic acid, where magnesium played another suppressive role. In fact, inhibition by magnesium was still observed even in the absence of nickel treatment. These results suggest that the protective role of magnesium in nickel-induced cytotoxicity and genotoxicity can be attributed to its ability to reduce either the intracellular nickel concentration or reactive oxygen formation.

Animals↗
PubMed1997

Cytotoxicity and multinucleate giant cell formation in Chinese hamster lung fibroblast caused by crocidolite and chrysotile.

The mechanism of carcinogenic action of asbestos remains unclear but the physical properties of the fiber appear to be important in this process. Asbestos may cause multinucleate giant cell formation primarily by interfering with the normal course of mitosis. We evaluated the cytotoxicity and multinucleate giant cell formation induced by crocidolite and chrysotile in Chinese hamster lung fibroblast (V79 cell) with observation of phagocytic activities. Asbestos fibers were rapidly ingested by V79 cells and most fibers were inside the cells. Cytotoxicity was evaluated by observing inhibition of V79 cell proliferation with trypan blue exclusion test. For determination of frequency of multinucleate giant cells, the cells were treated with different doses of crocidolite or chrysotile for 72 hours. Crocidolite and chrysotile induced cytotoxicity in V79 cells in a dose-dependent manner. The pattern of inhibition of cell proliferation is similar for both types of fibers, but chrysotile was more potent at the highest level (20.0 micrograms/ml) of fiber concentration. There was a good relationship (regression coefficientcrocidolite = 0.02, P < 0.01; regression coefficientchrysotile = 0.04, P < 0.01) between the dose of both asbestos fibers and the frequency of multinucleate giant cells. Chrysotile was again more potent at inducing multinucleate giant cells in higher levels of fiber concentrations. We found that asbestos fibers were cytotoxic after phagocytosis and induced multinucleate giant cells by interfering mitosis.

Animals↗
PubMed1995

[Determination of the progesterone antagonist lilopristone in serum by RP-HPLC].

To 1.0 ml of serum containing lilopristone were added RU486 solution (internal standard, IS) and 1 ml of 1.0 mol.L-1 NaOH. The mixture was extracted with diethyl ether for 2 times. After extraction, the combined organic phase was evaporated to dryness and the residue was dissolved in the mobile phase and washed with petroleum ether. After centrifugation, 20 microliters of the lower layer was subjected to HPLC. A muBondapak-C18 (10 microns) column (30 cm x 3.9 mm) was used and the column temperature was kept at 50 degrees C. The flow rate of mobile phase (methanol-dichloromethane--0.01 mol.L-1 phosphate buffer, pH 4.0, 67:5:28 v/v) was 1.1 ml.min-1 and UV detection was performed at 302 nm. The retention times of lilopristone and IS were 6.85 and 9.07 min respectively and the detection limit was 10 ng.ml-1 (S/N > or = 4) serum. The extraction recoveries of lilopristone and IS were over 85%. The relative standard deviations were 2.21 to 4.23%. This method has been applied to study the pharmacokinetic of lilopristone in rats.

Animals↗
PubMed1991

The periapical tissue reactions to a calcium phosphate cement in the teeth of monkeys.

A calcium phosphate cement, Grossman sealer, and Sargenti N2 were compared under conditions where root canals of monkey incisors were deliberately overfilled and the apical tissue responses were evaluated histologically. The periapical tissues exposed to Sargenti N2 revealed severe irritation at all times through the 6-month experimental period. The reactions to Grossman sealer were milder but persisted throughout the observation period. The calcium-phosphate-cement treated animals showed mild tissue irritation after 1 month, but thereafter the adverse tissue reactions were minimal. New bone formation adjacent to the cement was also observed. These results point to the possibility that calcium phosphate cement might be used in simplified endodontic procedures. The compatibility of calcium phosphate cement with the periapical tissue suggests that the cement may have other applications in dentistry and medicine.

Animals↗
PubMed1990

Prevalence of mottled enamel after 12 years of water fluoridation in Chung-hsing New Village.

The prevalence of mottled enamel (dental fluorosis) was investigated among children, aged 6 to 15 years, in Chung-hsing New Village and in Tsao-tun Village. The drinking water in Chung-hsing New Village has been fluoridated for 12 years at a level of 0.6 ppm, while the drinking water in Tsao-tun contains a negligible level of 0.08 ppm. The degree of mottled enamel was assessed by using Dean's Index and the Community Fluorosis Index (CFI), the latter being derived from Dean's Index for the purpose of comparison. In Dean's Index, 6 classes based on the severity of the mottling, are identified: normal, questionable, very mild, mild, moderate, and severe. The results showed that children in Chung-hsing had whiter teeth than those in Tsao-tun Village. In the fluoridated group, 5.8% of children fell into the "very mild" mottling category, as compared to 0.9% in Taso-tun, the control area. For the "mild" class, the rate was 1.9% for Chung-hsing New Village and 0.4% for Tsao-tun. With only one exception in Tsao-tun, none of the children in the two groups showed mottling in the "moderate" or "severe" classes. The Community Fluorosis Index (CFI) was 0.15 for the Chung-hsing group, indicating that the occurrence of mottled enamel is very low when compared with data from fluoridation programs in other countries. It is concluded that in Taiwan, a 0.6 ppm fluoride concentration in drinking water, which has previously been reported to be effective in prevention of dental caries, is associated with only negligible mottling of enamel. Even when mottling does occur, the degree is very mild and only small chalky spots can be seen on the teeth.

Adolescent↗
PubMed1990

Histologic reactions to a newly developed calcium phosphate cement implanted in the periapical and periodontal tissues.

A newly developed calcium phosphate cement (CPC), basically composed of tetracalcium phosphate, Ca4(PO4)2O and dicalcium phosphate dihydrate, CaHPO4.2H2O or anhydrous calcium phosphate, CaHPO4, was tested in this study. When combined with water, the cement hardens and produces hydroxylapatite resembling the principal mineral of teeth and bones. Therefore, the CPC should be highly compatible with the body. However, before its clinical application in dentistry, the biocompatibility of CPC in the potential environments of implantation needs confirmation. Eight monkeys were used in our study to test the material in the periapical and marginal periodontal regions. Surgically created periodontal defects were implanted with CPC and hydroxylapatite (HA) (Calcitite 4060). In another application, CPC was pushed into the periapical areas via overfilling of the root canals. Sargenti N2 served as the control material in the periapical test. Generally, only a limited inflammatory response to CPC was found after 6 weeks of implantation in the periodontal area. In the 16-week specimens, the adverse reaction was negligible, and bone regeneration was marked and directly surrounding the CPC. The bone formation activity and biocompatibility in general were found to be even better in the periapical region. The results suggest that the CPC may have a good potential in future clinical applications, although many issues remain to be investigated.

Animals↗
PubMed1989

Influence of water fluoridation on the eruption of permanent teeth in Chung-Hsing New Village, Taiwan.

Along with an evaluation of the effects of 12 years' water fluoridation in the prevention of caries, the present study was conducted to explore the influence of water fluoridation on the eruption of permanent teeth. The survey was carried out in fluoridated Chung-Hsing New Village and the control town of Tsao-Tun from October to December, 1984. Dental examinations were performed on 3,459 children Chung-Hsing New Village and 4,610 in Tsao-Tun, at ages ranging from 3 to 15 years. The results showed that in general, the mean tooth eruption time of girls was earlier than that of boys, and that teeth in the lower jaw emerge sooner than their homologues in the upper jaw, except for premolars. In both fluoridated and control areas the order of tooth eruption was very similar except for the teeth of girls in the upper jaw. The eruption sequence was first premolar, canine then second premolar in the girls of Chung-Hsing New Village, while first premolar, second premolar then canine in those of Tsao-Tun. By comparing the eruption time of permanent teeth in the children of fluoridated and control areas, it was found that they were very alike in general, the only difference being observed in the premolars of the sexes. The premolars of the children in the fluoridated area emerged in the oral cavity 2.5 to 4.5 months later than did their counterparts in the control area.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1985

Enhanced fluoride uptake from mouthrinses.

It has been shown in laboratory and animal studies that tooth enamel becomes considerably more reactive toward low levels of fluoride after receiving pre-treatment which forms dicalcium phosphate dihydrate (DCPD) in the enamel as an intermediate. This in vitro study was undertaken to determine the effect on human enamel fluoride uptake of incorporating DCPD-forming rinses into a conventional fluoride rinsing program. In one group, enamel samples received a one-minute rinse with 0.2% NaF each day for 20 days. Samples in the second group were treated identically, except that they were also given a one-minute DCPD-forming rinse prior to the first, sixth, eleventh, and sixteenth fluoride rinses. At the end of the rinsing program, all samples were washed in 1 M KOH to remove labile fluorides. The enamel biopsy data showed that the fluoride uptake resulting from 0.2% NaF alone was not statistically significant, whereas uptake produced by the DCPD-forming plus NaF rinses was significant. The results suggest that the DCPD-forming rinse may improve the efficacy of fluoride rinse programs.

Apatites↗