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Y C LEE

Publications and source records attributed to Y C LEE.

At least 19 recordsLinked to original sources
PubMed1964

USE OF A BACTERIAL CULTURE FILTRATE AS AN AID TO THE ISOLATION AND GROWTH OF ANEROBIC SPIROCHETES.

Hardy, Paul H., Jr. (The Johns Hopkins University School of Medicine, Baltimore, Md.), Young C. Lee, and E. Ellen Nell. Use of a bacterial culture filtrate as an aid to the isolation and growth of anaerobic spirochetes. J. Bacteriol. 87:1521-1525. 1964.-A previously described procedure that permits colonial growth of laboratory-adapted anaerobic spirochetes on surface streaked media was applied to the isolation of new spirochetal strains from human oral microflora. Primary isolates were readily cultivable as colonies, but they were found to be nutritionally dependent upon other microorganisms with which they are normally associated in vivo. Spirochetal growth was first noted around colonies of oral bacteria; pure cultures were then grown on media fortified with the culture filtrate of one such organism-a microaerophilic diphtheroid.

Bacteria↗
PubMed1963

COLONIAL GROWTH OF ANAEROBIC SPIROCHETES ON SOLID MEDIA.

Hardy, Paul H., Jr. (The Johns Hopkins University School of Medicine, Baltimore, Md.), Young C. Lee, and E. Ellen Nell. Colonial growth of anaerobic spirochetes on solid media. J. Bacteriol. 86:616-626. 1963.-A total of 14 strains of treponemes and one of Borrelia vincentii were cultivated as colonies on agar plates inoculated under ordinary atmospheric conditions, but incubated anaerobically. Every spirochete strain produced diffuse colonies with growth located primarily in the agar; in addition, eight treponeme strains also produced discrete colonies with surface growth. These represented stable colonial variants in several strains, and in one the spirochetes in the surface colonies showed permanently altered cellular morphology. Sufficient variation in colonial morphology was noted between strains to suggest that this may have some value in further classification of this group of microorganisms. Several conditions contributed to the successful growth of all strains as colonies, the most important of which was the use of a firm jelling agar preparation, Ionagar No. 2, that permitted preparation of plates with a final agar concentration of 0.7%. Agar concentrations of 0.9%, or above, in media inhibited treponeme growth.

Agar↗
PubMed1963

Innocent murmurs.

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Heart Auscultation↗