Biomedical subjects

Y F He

Publications and source records attributed to Y F He.

25 records · Page 2Linked to original sources

PubMed1994

Normal ranges for bone loss rates.

We reported previously that the variability in bone loss rates among postmenopausal women decreases dramatically during the first few years of followup. In this paper, we have examined the distributions of bone loss rates measured at the calcaneus, distal radius and proximal radius. The incidence of physical impairment was five times greater among women with bone loss rates faster than 2 S.D. below the mean. Because the rate of change in bone density was skewed at the lower end of the distribution (representing rapid bone loss), the influence of values at the extreme ends of the distribution were statistically removed in order to estimate the normal distribution of bone loss rates. For the convenience of clinicians, the upper and lower limits of the 90 and 70% normal ranges are presented. Because average bone loss rates vary with age, normal ranges are provided separately by age group. The width of each normal range decreased by at least half after 3 or 4 years of followup, compared to less than 1 year. Consequently, measured loss rates which were well within the normal range at 1 year were sometimes far outside the normal range for longer followup times. We conclude that followup duration has a profound effect on estimates of the normal range, and must be considered when interpreting the clinical significance of measured loss rates.

Adult↗

PubMed1993

A new method for vertebral fracture diagnosis.

A number of methods have been proposed for estimating the prevalence of vertebral fractures. Most methods are based on the distribution of normal vertebral dimensions in the population. However, these methods fail to identify a significant proportion (20-30% or more) of fractures documented on serial radiographs. This may occur because vertebral size varies between individuals as a result of differences in body size (and possibly other factors), and a normal range based on population reference data may be too large. In this paper, a new method is described for identifying vertebral fractures that are missed using diagnostic criteria based on vertebral dimension distributions of the population. This new method is based on calculating the average vertebral size, and statistical confidence limits, for the individual. The average vertebral size method was evaluated by testing its ability to identify incident fractures (which were identified from changes in dimensions compared to previous radiographs), using only the final film. The new method correctly identified most (81% of crush and 83% of wedge) incident fractures on the final radiograph. In contrast, criteria based on population distributions correctly identified only 53% of crush and 72% of wedge incident fractures. Using prospective data, prevalent fractures identified using both population-based and individual size-based criteria predicted the risk of incident fractures. Furthermore, incident fractures identified using both methods (population- and individual-based criteria) were associated with increased back pain. These data suggest that both types of prevalent deformities are important indicators of disease.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗

PubMed1993

Declining bone loss rate variability with increasing follow-up time.

Bone loss rates are believed to vary substantially among post-menopausal women. The belief, however, might be largely based upon comparisons of loss rates measured with considerable error. This issue arises because the precision of the bone mass instrumentation (1-2% errors in measuring bone mass) is similar in magnitude to the loss rate (typically 1-2% of bone mass per year). The 'true' variability in loss rates, however, can be estimated by adjusting for instrument errors. An equation is presented which estimates the true variability in loss rates from a study sample. The adjustment was examined using a cohort of post-menopausal, Japanese-American women living in Hawaii. The study examined the calcaneus, distal, and proximal radius sites. The results suggest that measurement errors did not markedly inflate the bone loss variability. Even after adjustment for measurement errors considerable variability in loss rates remained. The variability was examined for various follow-up durations. Both the observed and adjusted loss rates had smaller standard deviations over long intervals than over short intervals. This result suggests that the more extreme rates of change in bone mass over a year or two were often not sustained. The implications of this finding for sample size calculations in longitudinal studies are examined. The results also indicate that the length of follow-up and instrument precision should be taken into consideration when comparing the proportion of fast losers between populations.

Absorptiometry, Photon↗

PubMed1992

A method for estimating the uncertainty of future bone mass.

The development of statistical models for estimating fracture probability is a promising method for quantitating and optimizing the clinical utility of bone mass measurements. Earlier models have assumed that future bone mass could be predicted exactly and were, therefore, limited to analyses that assume the loss rate is known in advance. Since bone loss rates may vary over time and cannot be predicted accurately, we have developed a new model, based on empirical data, that estimates the degree of uncertainty associated with predicted bone mass. Without a bone mass measurement, the population mean must be assumed for an individual. For the calcaneus, the standard deviation of the population distribution is about 60 mg/cm2. By measuring bone mass, one can determine how close or far from the mean an individual's true bone mass is, with a standard deviation (SD) of about 3 mg/cm2. Without a subsequent bone mass measurement, our model predicts that the uncertainty (standard deviation) in calcaneal bone mass will increase approximately sixfold (relative to the reproducibility at the initial measurement) over a period of five years for women under age 60, from 3 mg/cm2 to 19 mg/cm2. The five-year increase in uncertainty is approximately fourfold for women over age 60, from 3 to 13 mg/cm2. However, the uncertainty in bone mass for an individual five years after the initial measurement is still only one third to one fifth that of the entire population, and can be reduced to the initial level by obtaining another measurement. Furthermore, the predicted (or measured) values are usually much better estimates of an individual's true bone mass than simply assuming the population average.(ABSTRACT TRUNCATED AT 250 WORDS)

Aged↗

PubMed1992

[Pattern visual evoked potential and pattern electroretinogram in patients with retinitis pigmentosa].

The pattern visual evoked potential (PVEP) was analyzed in 22 patients (42 affected eyes) with retinitis pigmentosa, and in 9 cases the pattern electroretinogram (PERG) was simultaneously examined to find that (1) PVEP could be recorded in 31 (73.8%) and was normal in 17 (40.5%) of the affected eyes; (2) PVEP was related to visual acuity, being abnormal in all the eyes with visual acuity below 0.2, but normal in most eyes with visual acuity over 0.2; (3) the changes in PERG and PVEP were mostly consistent; and (4) PVEP and PERG might well be utilized for the morbid evaluation of central retina in patients with retinitis pigmentosa.

Adolescent↗

PubMed1991

Increased DNA binding and transcriptional activity associated with transcription factor Sp1 in K562 cells transfected with the myeloid-specific c-fes tyrosine kinase gene.

Myeloblast cell line K562, when stably transfected with the human genomic c-fes sequence encoding a proto-oncogene tyrosine-protein kinase, acquires the characteristics of more mature granulocytic cells (WS-1 cells) and the ability to undergo differentiation (Yu, G., Smithgall, T. E., and Glazer, R. I. (1989) J. Biol. Chem. 264, 10276-10281). To explore the role of transcription factors in the differentiation process, WS-1 cells were analyzed for the presence of DNA-binding proteins capable of interacting with the 5'-long terminal repeat (LTR) region of human immunodeficiency virus (HIV)-1, that contains the binding sequences for transcription factors Sp1 and NFKB. Southwestern blotting and mobility shift assays revealed the presence of Sp1 in K562 and WS-1 cells. The DNA-binding activity of Sp1 was significantly greater in WS-1 cells than in K562 cells, despite the detection by immuno-blotting of equivalent quantities and degrees of heterogeneity of Sp1 in both cell lines. DNA footprinting of the HIV-1 5'-LTR demonstrated that two of the three Sp1-binding sites and both NFKB binding sequences were protected by nuclear extracts from WS-1 cells, while no protection was afforded by nuclear extracts from K562 cells. Analysis of transcription in vitro by primer extension revealed enhanced initiation of transcription from the HIV-1 5'-LTR by nuclear extracts from WS-1 cells, but not from K562 cells. These data indicate that the response evoked by the c-fes tyrosine-protein kinase leads to enhanced DNA binding activity of Sp1 and NFKB, that results in the activation of transcription from the HIV-1 5'-LTR.

Blotting, Southern↗

PubMed1991

[Effect of (-)-stepholidine on blood pressure and alpha-adrenoceptor agonists-, KCl- and CaCl2-evoked contractions of aortic strips].

Iv (-)-SPD lowered the blood pressure in anesthetized rat, the ED50 value was 5.1 +/- 2.5 mg.kg-1. In the experiments of rat and rabbit aortic strips, (-)-SPD 0.3-100.0 mumol.L-1 inhibited the contraction initiated by clonidine (alpha 2) and phenylephrine (alpha 1) and shifted the dose-response curve to the right parallely without change in maximum response. The inhibitory ratio of (-)-SPD acting on alpha 2/alpha 1 adrenergic receptors was about 7.2, and (-)-SPD thus was predominant inhibition on alpha 2 adrenergic receptors. In the experiment of aortic strips from reserpinized rabbits, the inhibition of (-)-SPD on contraction evoked by clonidine was diminished markedly. The results suggest that (-)-SPD stimulated mainly the alpha 2-adrenergic receptors of presynaptic nerve endings. Moreover (-)-SPD 1 mumol.L-1 inhibited the release of intracellular Ca2+ initiated by NE. (-)-SPD 3-30 mumol.L-1 blocked the voltage-dependent Ca2+ channel.

Animals↗