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Y Fontaine

Publications and source records attributed to Y Fontaine.

7 recordsLinked to original sources

Purification and properties of the DNA-binding protein HPB12 from Bacillus subtilis nucleoid.

We report the purification to homogeneity of a 12 KDa protein (HPB12) present in the nucleoids of Bacillus subtilis. From the purification data the abundance of the protein was estimated to about 20,000 monomers per cell. The HPB12 protein is heat-stable and acid-soluble and binds preferentially to supercoiled and linearized double-stranded DNAs. The binding of the protein to the supercoiled DNA occurs very rapidly and appears to be cooperative. Moreover, the complexes are extremely stable and do not dissociate after 90 min. These properties are consistent with a role of the HPB12 protein in the structure of the B. subtilis chromosome.

Bacillus subtilis↗

Transformation of a pericardial cyst observed on long-term follow-up.

An atypical pattern of pericardial cyst was observed on a routine chest radiograph of a healthy 25-year-old woman. It appeared as an important elevation of the right diaphragm. The cystic nature of the mass was easily disclosed by ultrasonography and computed tomography, but its location--thoracic, diaphragmatic, or subphrenic--remained questionable. Finally, a percutaneous puncture yielded 1300 mL of clear liquid. Despite this huge volume, the cyst had been asymptomatic and did not recur after complete aspiration. Retrospectively, owing to previous files, we could recognize the evolution of a pericardial cyst from the age of 13 years. At that time, it was much smaller and was evaluated by a series of aggressive investigations, although it had a more typical pattern and was confined to the right cardiophrenic angle.

Adult↗