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Biomedical subjects

Y Furuki

Publications and source records attributed to Y Furuki.

At least 19 recordsLinked to original sources
PubMed1997

A radiographic study of recurrent unicystic ameloblastoma following marsupialization. Report of three cases.

OBJECTIVES: To study the radiographic sequence in the recurrence of unicystic ameloblastoma following marsupialization. MATERIALS AND METHODS: Retrospective analysis of three cases of uncystic ameloblastoma. RESULTS: Six distinct stages were identified in all three cases: bone regeneration starting at the periphery of the cavity; appearance of a diffusely sclerotic band; appearance of scalloping; expansion of scalloping; formation of a multilocular pattern; and enlargement of the cystic lesion. CONCLUSION: The first radiographic sign of recurrence is scalloping of the sclerotic margin of the regenerated bone.

Adult↗
PubMed1991

Image salvage in panoramic radiography using histogram optimization.

Optical density and contrast recovery from poor quality panoramic radiographs was studied using the histogram modification technique. A series of experimental images of varying density and contrast were obtained by exposing a phantom in 4 kV steps between 50 and 94 kV. A standard of good image quality was selected by 10 observers grading the radiographs. Images were processed using the histogram specification technique and re-evaluated. All the processed images were found to be of acceptable quality. This technique was also shown to be applicable for salvaging unacceptable clinical radiographs.

Humans↗
PubMed1990

[Scoring system for screening of patients with endometriosis in infertile clinic].

An outpatient department screening method for endometriosis was prepared by designing a scoring system in a retrospective study. We compared the incidence of 21 characteristic symptoms and the finding of endometriosis in 39 infertile women with endometriosis diagnosed by laparoscopy or laparotomy and 21 women without it. A table for diagnostic scoring composed of 11 items was accomplished. The feasibility of this diagnostic scoring system was then examined in a prospective study of 72 women with endometriosis and 47 without it. The results were as follows. 1. In the retrospective study, all the women with 10 or more points had had endometriosis. Fifteen out of 20 women with -20 points or less had been without endometriosis and the remaining 5 with endometriosis (4 minimal and 1 mild stage according to AFS classification). 2. In the prospective study, a 94.5% (52/55) incidence of endometriosis was found in women with 10 or more points and 66.7% (10/15) of women with -20 points or less were without endometriosis. 3. This diagnostic scoring system, in which women with endometriosis score 10 or more points and women without it -20 points or less, has 91.2% sensitivity, 76.9% specificity, 94.5% positive predictive value and 66.7% of the negative predictive value. It is concluded that this scoring system has clinical diagnostic value in screening for endometriosis in infertile women at the outpatient department.

Adult↗
PubMed1989

[BANA hydrolase and DNP peptidase activities in the ovulatory process of PMS-hCG treated immature rat].

To investigate the roles of collagenolytic enzymes in the ovulatory process of PMS-hCG treated immature female rats (22 days old), we measured the activities of two of them in the ovary by the using synthetic substrates alpha-N-benzoyl-DL-arginine-2-naphthylamide HCl (BANA) (a collagenolytic cathepsin) and dinitrophenol-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg OH (DNP peptide) (a neutral collagenase). BANA hydrolase activities significantly began to increase after the hCG injection, reaching a peak 8-9 hours later, and then decreased sharply 10 hours later. There was also a significant increase in DNP peptidase activities 7-10 hours after the hCG injection and a significant decrease 12 hours after the injection. The present study has shown that BANA hydrolase and DNP peptidase appear during the ovulatory process of PMS-hCG-treated immature female rats, and that their significant preovulatory increases are contributory to collagenolysis in follicle rupture.

Animals↗
PubMed1988

Substrates for adenosine 3',5'-monophosphate (cAMP)-dependent protein kinase in the rat pituitary gland.

1. Substrates for cAMP-dependent protein kinase were investigated in anterior, intermediate, and neural lobes of the rat pituitary gland. In a cell-free assay system, cAMP increased phosphorylation of 17 K, 33 K, and 60 K macromolecules of the anterior lobe, 17 K, 33 K, 60 K, and 80 K macromolecules of the intermediate lobe, and 60 K, 80 K, and 85 K macromolecules of the neural lobe. 2. Other nucleotides were tested in the intermediate lobe; 8 Br-cAMP mimicked cAMP, cGMP was much less effective than cAMP or 8 Br-cAMP, and 5'-AMP showed no significant effect. The purified catalytic subunit of cAMP-dependent protein kinase evoked the same phosphorylation pattern as the endogenous kinase. 3. Maximum cAMP-dependent phosphorylation occurred at between 1 and 2 min of incubation; after 20 min, phosphorylation was reduced by 80%. This suggests the presence of phosphatase activity in the intermediate lobe. 4. When tested upon dispersed intermediate lobe cells permeabilized by high-voltage electrical discharges, cAMP increased phosphorylation of the 17 K and 33 K macromolecules.

Animals↗
PubMed1988

Radiographic characteristics of the calcifying odontogenic cyst.

4 additional cases of calcifying odontogenic cysts (COC) were reported, together with the radiographic interpretation of 138 cases from the literature. From the review of the literature and of our own cases, the root resorption is one of the most common diagnostic findings as is cystic radiolucency accompanied by radiopacities. Other radiographic characteristics, such as loculation, tooth divergency and bone resorption were emphasized.

Adolescent↗
PubMed1987

Alpha-melanocyte-stimulating hormone secretion from permeabilized intermediate lobe cells of rat pituitary gland. The role of guanine nucleotides.

The non-hydrolyzable GTP analogue, guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) and cyclic AMP potentiated the Ca2+-evoked secretion of alpha-melanocyte-stimulating hormone (alpha-MSH) from permeabilized neurointermediate lobe (IL) cells of rat pituitary gland. The enhancement by Mg-GTP gamma S (100 microM) and cyclic AMP (1 microM) depended on the intracellular Ca2+ concentration (EC50 = 4.8 +/- 1.8 and 4.6 +/- 1.7 microM; mean +/- SE, with and without Mg-GTP gamma S and cyclic AMP, respectively). A similar effect was observed with guanine nucleotide triphosphate (GTP and GppNHp). Mg was absolutely required for this event. Neither Mg-GTP gamma S nor cyclic AMP alone was effective in potentiating alpha-MSH secretion. GDP beta S blocked the Mg-GTP gamma S (100 microM) and cyclic AMP augmented secretion of alpha-MSH. Neither neomycin (which affects the process of inositol 1,4,5-triphosphate-mediated Ca2+ mobilization) or colchicine (which influences microtubule assembly) had an effect on the cyclic AMP and Mg-GTP gamma S potentiation of alpha-MSH secretion. These data suggest that the GTP-binding protein may be involved in the regulation of alpha-MSH secretion after Ca2+ entry into the cells, since the intracellular environment is controlled in the permeabilized cells.

Animals↗
PubMed1987

Adenosine 3',5'-cyclic monophosphate stimulates secretion of alpha-melanocyte-stimulating hormone from permeabilized cells of the intermediate lobe of the rat pituitary gland.

The effect of Mg-ATP and cyclic AMP on the secretion of alpha-melanocyto-stimulating hormone (alpha-MSH) from electrically permeabilized cells of rat intermediate lobe (IL) were investigated. Addition of exogenous Ca2+ stimulated alpha-MSH secretion in a concentration- (EC50 = 4.8 microM) and temperature-dependent manner. This Ca2+-evoked secretion was further enhanced by Mg-ATP and cyclic AMP. Mg-ATP was required for the fully secretory response in the electrically permeabilized IL cells and the maximal secretion was reached at 1 mM. Cyclic AMP in the presence of GTP gamma S also potentiated Ca2+-evoked alpha-MSH secretion to the same magnitude as Mg-ATP. In the absence of Ca2+ both the cyclic AMP and Mg-ATP did not stimulate alpha-MSH secretion from IL cells. The data suggest that Mg-ATP and cyclic AMP may modulate directly the secretory components rather than change intracellular concentration of free Ca2+.

Adenosine Triphosphate↗
PubMed1986

The effects of A23187 and phorbol ester on the phosphorylation of proteins in rat anterior pituitary cells.

The effects of A23187 and 12-O-tetradecanoyl-phorbol-13-acetate (TPA) on the phosphorylation of proteins in normal rat anterior pituitary cells were compared. A23187 rapidly activated the phosphorylation of at least 5 proteins (45, 47, 53, 54 and 58 kDa), which reached the maximal level in 2-10 min, and decreased gradually thereafter. In contrast, TPA activated the phosphorylation of at least 6 proteins (45, 62, 64, 72, 76 and 82 kDa), which were mostly distinguished from those activated by A23187. TPA-induced response was elicited more slowly, reached a plateau after about 10 min, and was sustained thereafter. These results suggest that the protein phosphorylation stimulated by A23187 and TPA is conducted by different mechanisms.

Animals↗
PubMed1985

[The effects of dopamine on the release of immunoreactive beta-endorphin-like peptide from the dispersed cells of the rat neurointermediate lobe].

The intermediate lobe of the rat pituitary gland is a homogeneous population of cells which synthesize and secrete various peptides related to ACTH and lipotropin derived from a common precursor, proopiomelanocortin. Catecholamine beta-receptor (beta-adrenoceptor) and dopamine receptor which are present in the intact cells of the intermediate lobe, remain functional in the enzymatically dispersed cells. In this study we investigated the mechanism by which beta-endorphin is released from the dispersed cells of the neurointermediate lobe of the rat pituitary gland. 1-Isoproterenol stimulated the release of immunoreactive beta-endorphin-like peptide (IR-beta-Ep) and the accumulation of adenosine 3', 5'-monophosphate (cAMP). On the other hand, dopaminergic drugs, apomorphine, bromocriptine, dopamine, lergotrile and lisuride, decreased the rate of release of IR-beta-Ep. Dopamine also inhibited the stimulatory effects of 1-isoproterenol on the release of IR-beta-Ep and cAMP accumulation. Dopamine antagonists, fluphenazine and sulpiride, diminished the inhibitory effects of dopamine on the release of IR-beta-Ep and cAMP accumulation which were stimulated by 1-isoproterenol. it has been reported that cholera toxin enhanced the release of IR-beta-Ep and the accumulation of cAMP in the rat neurointermediate lobe. After preincubation in the incubation medium containing 30 nM cholera toxin for 2 hours, the cells (CT cells) showed spontaneous release of IR-beta-Ep and cAMP accumulation. 1-Isoproterenol had no effect on the release of IR-beta-Ep and cAMP accumulation in CT cells. Dopamine, however, inhibited both the release of IR-beta-Ep from CT cells and cAMP accumulation in CT cells. These results suggest that dopamine may be involved in the regulatory mechanism of IR-beta-Ep release from the dispersed cells of the rat neurointermediate lobe.

Animals↗