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Biomedical subjects

Y Goussault

Publications and source records attributed to Y Goussault.

At least 37 records · Page 2Linked to original sources
PubMed1984

The cholesterol content of HDL2 and HDL3 subfractions of high density lipoproteins in different normocholesterolemic populations.

Two normocholesterolemic populations, selected for either high triglyceridemia or low HDL cholesterol content, both known to have increased artery disease risks, were studied for their cholesterol content in HDL2 and HDL3 subfractions. These subfractions were isolated by a precipitation method. The results showed that, in both populations, total HDL cholesterol values were similar and HDL2 and HDL3 cholesterol content were decreased when compared with a control population. In percentage of total HDL cholesterol, the HDL2 cholesterol subfraction appeared significantly diminished only in female subjects for both populations studied.

Adolescent↗
PubMed1984

Nature of the interaction between Ricinus communis agglutinin and blood cells.

Binding of Ricinus communis agglutinin (RCA 120) to carbohydrate receptors of human lymphocytes and erythrocytes is enthalpically driven. As in the case of simple saccharides, the delta S contribution is always unfavorable to the interaction. This result is different from that observed for other lectins and might indicate that hydrophobic interactions do not play a dominant role in binding of RCA 120 to cell surfaces.

ABO Blood-Group System↗
PubMed1984

Nature of the receptor sites for galactosyl-specific lectins on human lymphocytes.

The nature of the receptors for four lectins specific for D-galactosyl residues was examined in human lymphocytes. The cells were fixed with formaldehyde to avoid subsequent cell lysis, treated with pronase, sialidase and organic solvents, and the binding of the lectins to the treated cells measured. The results show that the bulk of the receptors for peanut agglutinin (PNA) and ricin (RCA 60) are glycoproteins, whereas those for Ricinus communis agglutinin (RCA 120) and soybean agglutinin (SBA) are distributed nearly equally between membrane glycoproteins and glycolipids.

Glycolipids↗
PubMed1984

Modification of biological activities of Ricinus communis agglutinin by cross-linking with formaldehyde.

Formaldehyde treatment of Ricinus communis agglutinin, a nonmitogenic toxic 120 000 molecular weight (MW) lectin, yielded two distinct protein fractions: one was heterogeneous and contained high molecular weight lectin polymers (greater than 120 000), and the other was a homogeneous 120 000 MW protein. Both fractions lost their cytotoxicity after formaldehyde treatment and stimulated thymidine incorporation into lymphocytes. Binding of both treated lectin fractions to lymphocytes exhibited positive cooperativity, whereas binding of untreated lectin did not.

Adult↗
PubMed1982

'Pseudohypertriglyceridemia' caused by hyperglycerolemia due to congenital enzyme deficiency.

A 76-year-old man was found to have a false hypertriglyceridemia due to a 40-fold increased glycerolemia. This metabolic change was due to a deficiency in glycerol kinase (ATP:glycerol phosphotransferase, EC 2.7.1.30) activity in the cells of this patient as shown by incubation of his white blood cells with [14 C]glycerol. Several chromatographic analyses and quantitative assays were performed on plasma and urine of this patient and of his relatives. The small number of this family's members did not allow to specify the mode of transmission of this genetic trait.

Aged↗
PubMed1982

[Xanthelasma palpebrarum and dyslipoproteinaemia: two retrospective studies (author's transl)].

In a retrospective study about 600 hyperlipoproteinaemic patients (390 men and 210 women, mean age 47), xanthelasma palpebrarum was infrequent (25 cases or 4.16 p. 100), since it was only at the third rank of chronic extra-vascular lipid deposits after arcus corneus and tendineous xanthomas. It was twice as frequent in men (5.12 p. 100) as in women (2.38 p. 100) and of regularly increasing occurrence until the sixth decade. Among the atherosclerotic risk factors, only obesity was significantly associated. The existence of atherosclerotic diseases was observed more frequently in patients with xanthelasma palpebrarum (40 p. 100) than in total hyperlipoproteinaemic population (28 p. 100). In a second retrospective study concerning 29 patients (15 men and 14 women, mean age 50), selected from the only presence of one or several xanthelasma palpebrarum, a lipoproteinaemic abnormality was observed in 27 cases (93 p. 100): 23 patients (71 p. 100) were hyperlipoproteinaemic (type IIa, IIb or IV) and in 4 out of 6 normolipidemic patients we observed an abnormal lipoprotein cholesterol distribution (LDL + VLDL cholesterol increase and HDL cholesterol decrease). In this group, we observed in nine patients (31 p. 100) unknown atherosclerotic diseases and tendineous xanthomas in other nine patients (31 p. 100).

Adolescent↗
PubMed1980

[Determination of the high density lipoprotein (HDL) cholesterol in blood serum. Comparison of 3 methods: ultracentrifugation and precipitations (author's transl)].

Three methods of assaying the high density lipoprotein cholesterol were applied simultaneously to a hundred of human sera: ultracentrifugation, precipitation by concavalin A and phosphotungstic acid. The obtained results were compared by statistical means: it was shown that the three procedures were equivalent. Some of their analytical features are discussed.

Chemical Precipitation↗
PubMed1980

[Comparative study of total cholesterol, HDL cholesterol, LDL + VLDL cholesterol. Two methods: precipitation by concanavalin A and ultra centrifugation (author's transl)].

The authors determined in 3 populations (37 controls, 59 asymptomatic hyperlipemias, and 20 cases of arterial hyperlipemia) the total cholesterol, the HDL cholesterol and the LDL + VLDL cholesterol and their ratio by two methods : precipitation by concanavalin A and ultracentrifugation (AirFuge). These various lipid parameters were compared between 3 populations in order to determine their more or less discriminant character; furthermore, was established the degree of correlation between the results obtained by precipitation with concanavalin A and by ultracentrifugation. The results show : firstly, that the LDL + VLDL was significantly higher and the ratio HDL/LDL + VLDL significantly lower in hyperlipemia and in arterial disease compared with controls ; the HDL was significantly lower in arterial disease than in controls. No lipid parameter permitted one to differentiate between asymptomatic hyperlipemia and arterial disease. Furthermore, the correlation determined by the chi square method was very strong between the results obtained by precipitation with concanavalin A and ultracentrifugation as the coefficient of simple linear regression is very little different from the value + 1. They emphasize the interest of the method of precipitation by concanavalin A which is simple, rapid and very reliable.

Adult↗
PubMed1979

Interaction between the Ricinus sanguineus agglutinin and receptor sites isolated from normal human lymphocytes.

Solubilized surface proteins from normal human lymphocytes were obtained by mild trypsin digestion. The binding of membrane components to labelled Ricinus sanguineus agglutinin (molecular weight = 120,000 daltons) was studied by a gel filtration method. The bound and unbound lectin amounts were determined from the gel filtration patterns. The binding parameters were calculated from Scatchard plots. They were compared to the parameters obtained at the same temperature for the lectin-intact lymphocyte system. The respective values for the affinity constant, were 3.5 x 10(6) M-1 and 6 x 10(6) M-1. The calculation of the number of sites per cell in each system specifies the yield of the trypsin digestion.

Cell Membrane↗
PubMed1979

[Modifications of peptide hydroxyprolinuria during Paget's disease and osteoma (author's transl)].

The values of hydroxyprolinuria were determined in 314 cases of Paget's disease and 91 cases of osteoma. During Paget's disease, the values found were related to the degree of the bony disorder. A more detailed study of the hydroxyproline polypeptides was undertaken, comparatively in a few cases of these two diseases. In Paget's disease, there was shown a relative increase in the oligopeptide fraction, whereas the polypeptide fraction was increased in osteoma. By chromatographic and electrophoretic technics, it was shown that the acid oligopeptides (containing mainly the dipeptide (Glu-Hyp) are in percentage, reduced in Paget's disease and increased in osteoma, whereas the neutral oligopeptides (containing mainly Pro-Hyp) undergo the opposite variation in these two diseases.

Female↗
PubMed1978

Activation of human B lymphocytes induced by Robinia pseudoacacia lectin in the presence of T cells.

Robinia pseudoacacia seed lectin is a potent human lymphocyte activator which is capable of activating pure T cells but not pure B lymphocytes. However, when B and T cells were cultured together, the thymidine incorporation was found to be higher than that expected from B- or T-cell cultures alone. Killing of T cells by anti-human-T-lymphocyte antigen (HTLA) serum and complement at the time of thymidine incorporation was found to be unable to suppress completely the thymidine uptake whereas treatment by anti-human-B-lymphocyte and monocyte antigen (HBLMA) serum reduced the response to some extent. Moreover, stimulated lymphoblasts were shown to bear B-cell markers (surface Ig and complement receptors) in about the same proportion as B lymphocytes present in the cultures. These results show that B cells proliferate in the presence of T cells and Robinia lectin. Finally, activation of B cells by Robinia lectin in the presence of T cells led to their maturation to plasma cells in the same way as PWM.

Antilymphocyte Serum↗
PubMed1976

Serum albumin biosynthesis and secretion by resting and lectin stimulated human lymphocytes.

Normal human peripheral lymphocytes, cultured in serum-deprived medium, synthetized and released serum albumin and some glycoproteins into the culture supernatant. With the use of [3H]leucine, it was shown that this biosynthetic activity was increased about 2-3 times when the mitogenic lectin from Robinia pseudo acacia was added to the lymphocyte culture medium.

Cells, Cultured↗
PubMed1976

Atilipidemic drugs. Part 5: Evaluation of the hypolipidemic effect of LF 178 in 191 patients affected by the atherogenic form of endogenous hyperlipoproteinemia (types IIa, IIb and IV).

The effect of isopropyl-[4'-(p-chlorobenzoyl)-2-phenoxy-2-methyl]-propionate (LF 178; procetofene; Lipanthyl), a new hypolipidemic agent, has been evaluated in 191 patients affected by endogenous hyperlipoproteinemia (IIa, IIb and IV). Data were collected from patients on active drug trial from 1 to 18 months. The daily administered dose varied from 200 to 400 mg according to pretreatment dietary stabilized lipid values. Under the controlled experimental conditions described, LF-178 lowered cholesterol by 20 to 36% in types IIa and IIb and triglycerides by 30 to 50% in types IIa and IV of H. L. P. A comparative trial of LF 178 and clofibrate indicates that the drug is significantly more potent than the reference compound after 1 month of treatment. Tolerance has always been excellent throughout the study.

Clinical Trials as Topic↗