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Biomedical subjects

Y H Chuang

Publications and source records attributed to Y H Chuang.

26 records · Page 2Linked to original sources
PubMed1991

Dystrophin immunostaining of muscle from Chinese patients with various neuromuscular diseases.

The localization of dystrophin was studied using the immunohistochemical method of diagnostic muscle specimens from 68 patients, aged 9 days to 65 years, with various neuromuscular disorders. Additionally muscle specimens from 2 normal humans and 2 normal mice were used as positive controls, and those from 2 mice with x-linked muscular dystrophy as negative controls. The specimens from all 14 Duchenne muscular dystrophy (DMD) patients, including one with preclinical DMD, showed negative dystrophin staining except for two which had 0.2% to 0.8% positive fibers. The mdx mice also showed negative dystrophin staining. In Becker muscular dystrophy (BMD), muscle fibers stained in a patchy or discontinuous fashion. Two symptomatic DMD carriers exhibited a distinct mosaic pattern of dystrophin positive and negative fibers. In contrast, dystrophin was present in all 7 biopsies from patients with 4 other types of muscular dystrophy (limb-girdle, congenital, myotonic and facioscapulohumeral). Other specimens, those from normal humans and control mice, revealed homogeneous immunostaining along the surface membranes of all muscle fibers. We thus conclude that immunohistochemical dystrophin staining can aid in differentiating DMD from preclinical DMD or BMD, as well as in the detection of DMD carriers.

Adolescent↗
PubMed1989

Acute effects of fever, fasting and aspirin on infant rat gastric mucosa.

Clinical experience shows that young children with gastrointestinal bleeding have frequently had some preceding febrile illness for which aspirin was administered. Febrile young children often have poor food or liquid intake, or have been in a fasting state because of diarrhea, vomiting or anorexia. The objective of this study was to determine the acute effects of fever, fasting and oral aspirin administration on the gastrointestinal mucosa. One hundred and sixty-eight infant rats, from 21 to 28 days of age and weighting from 70 to 120 g were studied. Random assignment was made to eight groups (Grs): Control (Gr I); aspirin administration only (Gr II); fasting only (Gr III); fever only (Gr IV); aspirin and fever (Gr V); fasting and fever (Gr VI); aspirin and fasting (Gr VII); and aspirin, fever and fasting (Gr VIII). Aspirin was given orally in a single daily dose of 200 mg/kg for two days. Fever was induced by an intraperitoneal injection of 0.6 ml salmonella vaccine. Fasting time lasted from 40 to 48 hours (8 hours prior to the beginning of the experiment to the end of study). The severity of the gastric bleeding was estimated by scoring the number of petechiae and the percentage of the hemorrhagic erosion area from grade 0 to 3. Results showed that rats in Grs VII and VIII had significantly more severe grades of petechiae and hemorrhage than the other groups. These were the groups where the risk factors of fasting and aspirin administration coexisted. In addition to fasting, Gr VIII had fever, but this group did not show more gastric mucosal damage than Gr VII showed.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMedDate not supplied

Change of chemokines during immunotherapy in asthmatic children.

Histamine-releasing factor (HRF) consists of a group of cytokines that can cause basophil/mast cell to release histamine, however, the composition of HRF still remains undefined. This study was designed to measure the concentrations of chemokines in asthmatic children receiving immunotherapy. Peripheral blood mononuclear cells culture supernatants were obtained from six asthmatic children before and four, eight months after immunotherapy (IT). The levels of monocyte chemotactic and activating factor (MCAF), macrophage inflammatory protein-1a (MIP-1a), regulated on activation normal T-cell expressed and secreted (RANTES) and interleukin-8 (IL-8) spontaneously and after stimulation with PHA and mite allergen in the supernatants. The data showed: 1) The levels of MCAF and MIP-1a increased four months, and decreased eight months, after IT; 2) By contrast, the level of RANTE increased after IT; 3) The level of IL-8 also tended to increase after IT. Abnormal chemokine production may contribute to the pathogenesis of bronchial asthma and restoration of normal chemokine production may be used to partially explain the clinical efficacy of immunotherapy.

Adolescent↗