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Biomedical subjects

Y Handa

Publications and source records attributed to Y Handa.

At least 217 records · Page 12Linked to original sources
PubMed1984

Analyses of lysophosphatidic acids by gas chromatography mass spectrometry without hydrolytic pretreatment.

Lysophosphatidic acids having different fatty acyl moieties were directly analysed by gas chromatography/mass spectrometry after silylation. Trimethylsilyl derivatives of various LPAs escaped thermal degradation, and electron impact and chemical ionization of these derivatives yielded many ions which were useful for the structural determination. It was found by this new method that five species of lysophosphatidic acids were formed in rat plasma during incubation at 37 degrees C.

Animals↗
PubMed1984

Chondroma of the tongue. Report of a case.

A case of chondroma which occurred in the midline of the dorsum of the tongue in a 40-year-old Japanese male is reported together with a review of the literature. Histological investigation revealed that this lingual chondroma had the characteristics of hyaline cartilage. We regard the metaplastic transformation theory as the best explanation of histogenesis of lingual chondroma at the lateral margin, while cartilaginous rests are the origin of lingual chondroma on the dorsum of the tongue.

Adult↗
PubMed1984

Phosphorus-free antihypertensive lipid from dog peritoneal dialysate, Peritoneal Dialysate Depressor-II.

A phosphorus-free lipid with a transient antihypertensive effect was found in a dog peritoneal dialysate by a modified procedure. On silicic acid chromatography of the crude extract, a new hypotensive factor and a new hypertensive factor were separated besides the known antihypertensive phospholipid resembling lysolecithin (Peritoneal dialysate depressor-I). The new antihypertensive factor was purified almost completely by gel filtration on a Sephadex LH-20 column, and found to contain no phosphorus. It was tentatively named Peritoneal Dialysate Depressor-II. The main spot detected on preparative thin-layer chromatography by charring with ethanolic sulfuric acid reagent was found to be responsible for the antihypertensive action. This factor caused a sharp decrease in systemic arterial blood pressure of rats, cats and guinea pigs when injected into the femoral vein. There was no significant change in the heart rate during this hypotension. The hypotensive activity was not affected by previous treatment of the rats or guinea pigs with antimuscarinic, antihistaminic, beta-adrenergic-blocking or ganglionic-blocking agents. The antihypertensive effect of the factor was unaffected on rats pretreated with yohimbine, indomethacin or theophylline. In spinal or reserpinized rats, no detectable reduction of the depressor activity was observed. The factor did not affect isolated guinea pig ileum. Its depressor activity was not changed significantly by its treatment with proteases. No prostaglandin E or F series compounds were detected in the preparation by fluorometric assay with 15-hydroxyprostanoate oxido-reductase and resazurin. The hypotensive factor caused no aggregation of cat or rabbit platelets.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1984

Electrophysiological and morphological cell polarity and iodine metabolism in cultured porcine and human (normal and Graves') thyroid cells.

In cultured porcine and human thyroid cells, electrophysiological and morphological studies showed that cultivation in the presence of TSH, prostaglandin E2 (PGE2) or dibutyryl cyclic AMP (dbcAMP) maintained normal cell polarity with iodine incorporation and organification. Cells cultivated in the absence of these substances had inverted cell polarity and lacked iodide incorporation. In the presence of TSH, PGE2 or dbcAMP, the thyroid cells formed follicles with normal cell polarity and the microvilli pointed toward the follicle lumina. Intracellular resting potentials were -60mV and the electrical potentials in the follicle lumina were negative at -20mV. The transmembrane potential differences (p.d.) between the follicle lumina and the epithelial cells were -40mV and those between the epithelial cells and the culture media -60mV. In the absence of TSH, PGE2 or dbcAMP, the thyroid cells formed 'domes' or hollow spheres with inverted cell polarity and the microvilli pointed toward the culture media. Intracellular resting potentials were -40mV, being less negative than those in the presence of TSH, PGE2 or dbcAMP. The electrical potentials in the 'dome' or hollow sphere cavities were positive at +19mV. The transmembrane p.d. between the culture media and the epithelial cells was -40mV and that between the epithelial cells and the cavities -60mV, indicating that electrophysiologically the cell polarity was inverted in the absence of TSH, PGE2 or dbcAMP. No significant differences in electrophysiology and iodine metabolism were observed between normal and Graves' human thyroid cells in culture.

Animals↗
PubMed1984

[Note on the mechanism of producing the plateau waves].

Isotope cisternography was performed and ventricular system size was evaluated on serial computerized tomography scans in 57 patients with plateau waves in their continuous intracranial pressure recordings. The patients were assigned to three groups based on the presence or absence of communicating hydrocephalus and their intracranial pressure level: the first group comprised 23 patients with high intracranial pressure and without hydrocephalus, resulting from supratentorial brain tumor, benign intracranial hypertension and superior sagittal sinus thrombosis; the second group included 22 patients with either high intracranial pressure or communicating hydrocephalus, resulting from intracranial aneurysmal rupture: and the third group was composed of 12 patients with normal intracranial pressure and communicating hydrocephalus, resulting from intracranial aneurysmal rupture. Plateau waves in the first group patients were characterized as large plateau like formations recurring at varying intervals, with a height of 50-100 mmHg and a duration of 10 to 30 minutes. Plateau waves in the second group patients had a height of 20 to 60 mmHg and a duration of 3 to 15 minutes. The waves in the second group patients were smaller in amplitude and shorter in duration as compared with the waves in the first group patients. Plateau waves in the third group patients had an amplitude of 15 to 40 mm Hg and a duration of 3 to 10 minutes. They were the smallest in amplitude and the shortest in duration as compared with the plateau waves seen in the first and the second group patients.(ABSTRACT TRUNCATED AT 250 WORDS)

Brain Neoplasms↗
PubMed1984

[Clinical studies on preoperative abdominal arterial infusion of anticancer agents].

Operable patients with gastrointestinal cancer were injected once with MMC 0.4 mg/kg, ADM 0.8 mg/kg or KW 2083 1 mg/kg through the abdominal artery 7 to 15 days before operation. Biopsy was made on liver tissues during operation, and changes of serum biochemical test values were observed before and after operation. In 7 cases of MMC 20 mg arterial infusion, 7 cases of ADM 40 mg and 6 cases of KW 2083 50 mg liver biopsy showed fibrosis around the central vein. Eosin bodies, neutrophils or necrotic lesions were observed in some other cases. Serum biochemical test values were transiently affected with anesthesia, but these changes will have no unfavorable influence on the results of operation and following treatment.

Abdomen↗
PubMed1984

[Intracranial benign chondroblastoma. A case report and review of the literature].

A case of benign chondroblastoma extending from the nasal cavity to the frontal region, was reported. A 1-year and 9-month old girl was admitted to our hospital in April 1981 because of generalized convulsion. On admission, she was intact neurologically and had It. nasal obstruction and It. eye discharge. Her laboratory examinations, including serum electrolytes and A1-P, were all in normal range. Plain skull X-P showed marked calcification from midfrontal region to the nasal cavity, and destruction of the frontal base especially in It. side. Plain CT showed isodensity mass with spotty calcification in the nasal cavity and marked high density mass in the frontal region. By contrast enhancement, nasal mass was markedly enhanced and new enhanced mass appeared around the intracranial calcification. Lt. ext. CAG showed the enlarged middle meningeal artery which is the feeder of the tumor, fine tumor vessels and slight tumor stain. Lt. int. CAG only showed the mass effect. After three-stage operation of bifrontal craniotomy, sublabial transnasal approach and rhinotomy, all the tumor was removed and she was discharged with no neurological deficits. The tumor was well demarcated and consisted from solid, cystic and calcified region. The solid region was grayish and easy-hemorrhagic. Histological examination revealed characteristic features of benign chondroblastoma, such as diffuse proliferation of polygonal or round cells, foci of chondroid matrices, scattered polynuclear giant cells and some mitosis.

Brain Neoplasms↗
PubMed1984

[Regional cerebral blood flow and neurological conditions in patients with surgically treated hypertensive intracerebral hemorrhage].

Regional cerebral blood flow (rCBF) was measured in 49 patients with surgically treated hypertensive intracerebral hemorrhage (putaminal hemorrhage 27: thalamic hemorrhage 22). The hematoma evacuation was performed within 14 days after the hemorrhage. For the measurement of rCBF, the intracarotid xenon-133 injection method was used. Two rCBF studies were performed on all patients, the first giving the resting state of the brain perfusion, the second after Prostaglandin E1 (PGE) administration into the internal carotid artery 10 minute after the first study. (PGE was administered 10 micrograms in 5 minutes.) In all patients blood pressure and arterial carbon dioxide tension were examined before and after each CBF study. The evaluation was made by comparing the rCBF maps obtained before and after PGE administration. (A rate of rCBF increase was calculated by dividing each rCBF value obtained after PGE administration by each rCBF value obtained before PGE administration (control value), and the ratio was multiplied by 100.) The PGE-induced reaction patterns were divided into four; the first is the diffuse increase pattern, characterized by a diffuse increase of rCBF values in the hematoma-evacuated area and the surrounding brain; the second is the diffuse decrease pattern, characterized by a global decrease of rCBF values in the hematoma-evacuated area and the surrounding brain; the third is the pattern of intracerebral steal effect, characterized by a decrease of rCBF in the hematoma-evacuated area and an increase of rCBF values in the surrounding brain; and the fourth is the pattern of inverse cerebral steal effect, characterized by an increase of rCBF in the hematoma-evacuated area and a decrease of rCBF in the surrounding brain.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1983

[Left ventricular diastolic performance in patients with myocardial infarction: assessment with backward method of radionuclide angiography].

Radionuclide technique in the evaluation of left ventricular (LV) diastolic performance has been applied in patients with myocardial infarction (MI). But the late diastolic LV volume curve obtained from the radionuclide angiogram is unreliable, whenever we sum up radioactivities by ECG-triggered method (conventional method) because of respiratory arrhythmia. The purpose of the present study was (1) to estimate the reliability of diastolic LV volume curve obtained from the radionuclide angiogram by our new method (backward method: backward ECG-gated radionuclide angiogram to the preceding R wave), and (2) to determine the availability of Phase 1 max and Phase 3 max as the indexes of LV diastolic performance. We analyzed LV volume curve and its dV/dt curve in 29 cases by both conventional and backward methods. The LV diastolic period was equally divided into three time intervals. The early, mid and late trisections of the diastolic period were expressed as phase 1, phase 2 and phase 3, respectively. A Phase max was defined as maximum dV/dt in the phase corrected for end-diastolic counts. At first, ejection fraction (EF), Phase 1 max, Phase 2 max and Phase 3 max were compared between the two different methods to estimate the reliability of diastolic LV volume curve obtained from a backward method. The backward method correlated well with the conventional method regarding EF and Phase 1 max (r = 0.965 and r = 0.940, respectively), but there was no correlation regarding Phase 3 max. This indicated that LV diastolic volume curve obtained from the backward method was reliable. In the second place, we analyzed LV dV/dt curve obtained from the backward method in 8 controls and 28 MI patients to determine the availability of Phase max as the indexes of LV diastolic performance. Phase 1 max was reduced earlier than a decrease of EF, and Phase 3 max was increased prior to a decrease of EF in MI. These data indicated that Phase 1 max and Phase 3 max as the indexes of LV diastolic performance were more sensitive than the indexes of LV systolic performance. Also MI patient had an abnormality in early diastolic filling and an increased atrial contraction in late diastolic filling of the left ventricle. The mechanism of abnormal LV early diastolic filling, even in the absence of abnormal systolic function, might be impaired LV suction because of fibrosis or the relatively ischemic myocardium. In mild to moderate LV failure atrial contraction compensated abnormal early diastolic filling, but there was no such a compensation in severe LV failure. It may suggest the limitation of atrial function as a booster pump.

Aged↗
PubMed1983

[Clinical application of a new right subcostal approach in the evaluation of interatrial septal motion].

We developed a new echocardiographic approach to detect the interatrial septum (IAS) by tilting a transducer leftward, cephalad and backward from the right subcostal area. This technique could allow us to visualize the IAS moving perpendicularly to the ultrasonic beam. In normal subjects the IAS showed a small posterior deflection moving toward the left atrium due to atrial contraction following the P wave of the electrocardiogram. Following the onset of ventricular ejection the IAS rapidly moved anteriorly. During diastole the IAS showed an initial rapid posterior displacement and then a more gradual slope, reflecting rapid and slow filling phases, respectively. The magnitude and configuration of IAS motion showed variations dependent on atrial conditions of cardiac diseases. In atrial fibrillation there were f waves on the IAS echogram and in mitral stenosis a septal notch was recorded at the timing of a mitral opening snap. In mitral valve prolapse there was also a midsystolic notch of the IAS echogram almost coincident in time with the onset of a late systolic murmur. On the other hand, the IAS revealed a systolic increased excursion or paradoxical motion in mitral or tricuspid regurgitation, respectively. In hypertension and myocardial infarction the atrial kick of the IAS echogram showed an exaggerated excursion. In 125 out of the consecutive 150 cases (83.3%) we could record satisfactory IAS echograms by this new approach.

Adolescent↗