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Y Hayase

Publications and source records attributed to Y Hayase.

At least 73 records · Page 4Linked to original sources
PubMed1992

Partial purification and characterization of a factor for the enhancement of colony formation in vitro by myeloid progenitor cells.

We have purified a factor, hematopoietic promoting factor (HPF), from porcine kidney extract (PKE), which exhibits a promoting activity on granulocyte/macrophage (GM) colony and burst-forming-unit-erythroid (BFU-E)-derived colony formation by progenitors from murine bone marrow cells in vitro. The addition of HPF resulted in an enhancement of the GM colonies as well as BFU-E-derived colonies, but did not enhance the colony-forming-unit-erythroid (CFU-E)-derived colony formation. HPF was added to the BFU-E cultures together with cytokines, such as recombinant murine interleukin-3 (IL-3), recombinant murine GM colony-stimulating-factor (GM-CSF) and recombinant human G-CSF, which have all been shown to enhance BFU-E growth. The combination of HPF plus these cytokines resulted in an enhancement of benzidine negative colony formation in comparison to the case of each cytokine alone; however, no increase was found on BFU-E colony formation. HPF is able to enhance the granulopoiesis and erythropoiesis in vitro. And the synergistic activity of HPF is significantly affected by the presence of cytokines in the cultures.

Animals↗
PubMed1991

Comparative studies of the colony-promoting activity of porcine kidney extract with several interleukins and colony-stimulating factors.

Porcine kidney extracts (PKE) possess colony-promoting activity (CPA) which stimulates primitive hematopoietic cells in the presence of granulocyte/macrophage colony-stimulating factor (GM-CSF), but PKE itself does not stimulate colony formation on murine bone marrow cells. We have compared the CPA of PKE with that of recombinant cytokines or CSFs such as interleukin-1 alpha (IL-1 alpha), IL-3, IL-6, granulocyte colony-stimulating factor (G-CSF), GM-CSF and macrophage colony-stimulating factor (CSF-1). All of these factors were less potent than PKE. Furthermore, the combinations of IL-1 alpha or PKE with G-CSF, GM-CSF, IL-3 or IL-6 were examined in the presence of one of these factors such as CSF. It is found that PKE acts synergistically with G-CSF, GM-CSF, IL-3 and IL-6, showing enhancement ratios of 10, 2.5, 4.2 and 30, respectively. The combination of IL-1 alpha resulted in poor colony formation in contrast with those of PKE, except for CSF-1. These results suggest that the CPA of the factor(s) in PKE differ from the cytokines and CSFs tested in this study, and is significantly affected by various types of CSF.

Abdominal Muscles↗
PubMed1991

Enhancement of murine erythropoiesis in vitro by a porcine kidney extract.

We have examined the effect of porcine kidney extract (PKE) on the growth of erythroid progenitor-derived colonies in a methylcellulose culture. The addition of PKE resulted in an enhancement of burst-forming-unit-erythroid (BFU-E)-derived colonies, and the enhancement of the colony was also observed in a low concentration of erythropoietin (Epo) and fetal calf serum (FCS). The activity of PKE on BFU-E was compared with the erythroid growth factors, such as recombinant murine interleukin-3 (IL-3), recombinant murine granulocyte/macrophage colony-stimulating factor (GM-CSF) and recombinant human granulocyte colony-stimulating factor (G-CSF) which are all well known to stimulate BFU-E growth. IL-3 showed a potent burst-promoting activity (BPA), but GM-CSF and G-CSF did not enhance BFU-E growth in our culture conditions. In the cultures supplemented with Epo, the rapid loss of BFU-E was prevented with IL-3; however, PKE alone did not prevent the disappearance of BFU-E. These results suggest that PKE possesses a BPA-like activity which is considered an enhancement of BFU-E.

Animals↗
PubMed1991

Properties of colony promoting activity in procine kidney extract.

Aqueous extracts prepared from procine kidneys (PKE) possess colony-promoting activity (CPA) which increases the number of granulocyte and macrophage colonies in semi-solid cultures of mouse bone marrow cells (BMC) in the presence of colony-stimulating factor (GM-CSF). PKE was totally inactivated by 15 mM N-ethylmaleimide, but was resistant to 5 mM dithiothreitol (DTT), 50 mM sodium metaperiodate and a mixture of diisopropylether-n-BuOH (3:2). The proportion of deoxyribonucleic acid (DNA)-synthesizing cells of the PKE-responsive cells was about one-half in comparison to those of CSF-responsive cells, as estimated using the hydroxyurea (HU) suicide method. Upon marrow preincubation with PKE in liquid culture for 24 h, the suicide rate of the colony forming unit in culture (CFU-C) by HU increased to 3 times compared to that of the control. Since cyclophosphamide (CY) induces a change in the number of CFU-C, the effects of PKE on BMC obtained from CY injected mice were investigated. On day 1, the number of PKE-responsive cells significantly increased by about 2.3 times in comparison with that of control, whereas the number of CFU-C per 1 x 10(4) cells significantly decreased to about one-eighth of that of control. These results suggest that a sulfhydryl group(s) is required for the appearance of the colony-promoting activity of PKE, and glycoproteins, glycopeptides or hydrophobic components are not required; they also suggest that PKE may act on immature granulocyte/macrophage progenitors, which are younger than CSF-responsive CFU-C.

Animals↗
PubMed1990

Secondary structure in formylmethionine tRNA influences the site-directed cleavage of ribonuclease H using chimeric 2'-O-methyl oligodeoxyribonucleotides.

In order to cleave RNA at specific positions in Escherichia coli formylmethionine tRNA, RNase H and complementary chimeric oligonucleotides consisting of DNA and 2'-O-methyl-RNA (Inoue et al. (1987) FEBS Lett. 215, 327] were used. Specific cleavages in the D loop, anticodon loop, T psi C loop, anticodon stem, and acceptor stem were investigated. Virtually unique hydrolyses with RNase H were observed at the T psi C loop, anticodon stem, and acceptor stem when relatively longer chimeric oligonucleotides (20-mer) were used. An efficient cleavage at the anticodon was obtained with a chimeric 13-mer when the higher structure of the tRNA was broken by hybridization with a 20-mer at the acceptor as well as the T psi C stem region. It was found that stabilities of hybrids with chimeric oligonucleotides and the presence of minor nucleosides affect the cleavage of tRNA by this approach.

Anticodon↗
PubMed1990

Protein analysis of newly isolated variants of echovirus type 18 by electrophoresis and western blotting.

The protein patterns of two newly isolated antigenic variants of echovirus type 18 were compared with those of the prototype strain. Electrophoretic mobilities of VP1 and VP2 of the variants differed from those of the prototype strain. The antiserum against the prototype strain reacted to VP0 and VP2 in the Western blotting experiment. In addition, the variants differed from the prototype strain in growth behavior in the cultured cells.

Blotting, Western↗
PubMed1990

Surveying cis-acting sequences of pre-mRNA by adding antisense 2'-O-methyl oligoribonucleotides to a splicing reaction.

We chemically synthesized antisense 12 mer 2'-O-methylribonucleotides and surveyed a scanning (signal-tracking) process as well as sequences within a beta-globin transcript acting in the splicing reaction in vitro. The pre-mRNA transcript contained the sequences of the first exon, first intron, and a major part of the second exon of the human beta-globin gene. We found that the antisense 2'-O-methylribonucleotides could anneal effectively to the target site in the pre-mRNA during the splicing reaction. A 2'-O-methylribonucleotide complementary to the donor (5') splice site completely inhibited authentic splicing and activated an upstream cryptic donor site. A 2'-O-methylribonucleotide complementary to the branch site inhibited normal branch formation and greatly reduced subsequent generation of the spliced product. Six other 2'-O-methylribonucleotides complementary to loci in the exons or the intronic region between the donor and branch sites had no significant effect on the splicing reaction. These observations suggest that an extensive scanning of the present pre-mRNA across the six regions tested is not essential for the splicing reaction. We propose that a short antisense 2'-O-methylribonucleotide provides a practical and convenient method to examine cis-acting sequences of RNA. The advantages of this method in comparison with site-directed mutagenesis or deletion are discussed.

Base Sequence↗
PubMed1990

An acoustically investigated case of phonetic change in higher vertical dimension.

This report presents the phonetic changes of a patient whose occlusal vertical dimension is extended. His chief complaint is esthetic restoration of his anterior teeth, but his occlusal vertical dimension is abnormally shortened by long-time attrition. Therefore we extended his occlusal vertical dimension, and reconstructed his occlusion, and carried out acoustic observations.

Aged↗
PubMed1989

Design of RNA enzymes distinguishing a single base mutation in RNA.

RNA enzymes (ribozymes) which can cleave RNA by recognizing sequences of 9-15 bases are described. Substrates must contain UX (X = U, C or A). A ribozyme consisting of two oligoribonucleotides (19 mer and 15 mer) was shown to cleave a ribo 11 mer catalytically with Km and kcat values of 0.53 microM and 0.03 min-1, respectively. A non-cleavable substrate-ribozyme complex containing 2'-O-methylnucleoside was prepared and CD spectra were compared at different temperature. In order to obtain an efficient ribozyme, a one-strand RNA with a chain length of 37 was prepared. The ribozyme was shown to distinguish a single base mutation in mRNA's which were prepared by transcription of two synthetic DNA duplexes coding for positions 7-26 of c-Ha-ras protein. The mutant (Val-12) mRNA which had GUU was cleaved but the wild type mRNA which contained GGU was not changed, when treated by the ribozymes in the presence of Mg2+.

Base Sequence↗
PubMed1989

[PARCOR analysis in experimental plate wearer's sound and its application to evaluation of plate formation].

The study demonstrates the relationship between palatal formation and human utterance by PARCOR analysis. PARCOR (partial autocorrelation) coefficient 'k' in speech wave was derived from the conception of linear prediction of human speech production by ltakura. Linguistic opposition was also adapted for the evaluation of plate forms and postions. Investigations were conducted to six adult men as the subjects who wore the experimental plates divided by four steps. PARCOR coefficients of the plate wearer's sounds were shifted as the plate area increase, and linguistic oppositions were individually proposed. Conclusions were represented as follows; Phonetic opposition of /p/X/m/ and /d/X/m/ (in 'stop' to 'nasal' opposition) were influenced by the width of the plates. Opposition of /t/x/s/ (in 'stop' to 'fricative' opposition) was significantly influenced by the frontal positions in oral cavity. Phonetic oppositions were also influenced from vowel environments to the consonants. As the plate areas were increased in order of full plate, 1/2 plate and 1/4 plate, the evidency of the oppositions were decreased.

Denture, Complete, Upper↗
PubMed1989

[Acoustic investigation of various palatal rugae].

We use some palatal plates with some Kinds of palatal rugae; ready made, patterned, individually copied and omitted, and studied the influence of the pronunciation acoustically concerning the Formant Frequency.

Dental Impression Technique↗
PubMed1989

Design of RNA enzymes for sequence-dependent cleavage of RNA.

We have designed synthetic ribozymes which can cleave specific sites of RNA. An undecamer, 5S rRNA and transcripts using T7 RNA polymerase were hydrolyzed at the X position of RNA containing (N)nUX(N)n (N = A, G, C and U; X = A, C and U) by these ribozymes.

Base Sequence↗
PubMed1988

Lysosomal enzyme activities in the cerebral microvessels in spontaneously and renal hypertensive rats.

In an attempt to clarify the role of lysosomal enzymes in the developmental mechanisms of the changes of cerebral microvessels under hypertensive conditions, the activities of acid phosphatase, N-acetyl-beta-glucosaminidase, and beta-glucuronidase in the isolated microvessels from the cerebral cortex of spontaneously and renal hypertensive rats were biochemically studied. The activities of all the enzymes were higher than those in normotensive control animals, although there was a variation in intensity according to the age and kind of enzymes. The enzyme activities in spontaneously hypertensive rats showed a tendency to increase with advancing age. Hypertension seems to increase activities of lysosomal enzymes in cerebral microvessels, and this activation may in turn play a role in the development of further hypertensive cerebrovascular and cerebral changes.

Acetylglucosaminidase↗
PubMed1988

Effect of hypertension on lysosomal enzyme activities in aortic endothelial cells.

In order to obtain information about the changes in lysosomal enzyme activities in arterial endothelial cells under hypertensive conditions, a biochemical study was performed on 5 lysosomal enzymes, acid phosphatase, N-acetyl-beta-glucosaminidase (NAGase), cathepsin B, cathepsin D and beta-glucuronidase, in endothelial cells isolated by an enzymatic technique from the aorta of spontaneously and renal hypertensive rats, and normotensive control rats. The aortic endothelial cells in the old spontaneously and the renal hypertensive rats showed increased activities of enzymes examined in comparison with those in the age-matched control rats. Endothelial cells in young spontaneously hypertensive rats did not show any elevated enzyme activities compared with those in the controls, and the enzyme activities tended to increase with aging. From this, it is deduced that hypertension activates lysosomal enzyme activities in aortic endothelial cells. The differences in the activities of NAGase, cathepsin B and cathepsin D between hypertensive and control animals increased markedly with advancing age. These activated lysosomal enzymes seem to be involved in the developmental mechanism of arterial endothelial cell injury in hypertension and in further development of hypertensive vascular changes.

Acetylglucosaminidase↗
PubMed1988

Sequence-specific cleavage of RNA using chimeric DNA splints and RNase H.

To cleave RNA molecules using E. coli RNase H in a site-specific manner, a short oligodeoxyribonucleotide (3-5 mer) linked with oligo(2'-O-methyl)ribonucleotide(s) was designed to be used as a DNA splint. Our model experiments with ribooligomer the splint duplexes (9 mers) and RNase H demonstrated that a tetradeoxynucleotide cluster seems to be sufficient for the enzyme recognition and the short DNA-containing splint directs a unique cleavage of RNA by RNase H. The method could be applied to longer ribooligonucleotide substrates. For example, when 3'm (GA)d(AGAA)m(GGU)5' was used as a hybridization strand, 32pUCUUUCUUCUUCCAGGAU was cleaved specifically between U11 and C12 to yield 32pUCUUUCUUCUU. This method will have a variety of applications for the study of RNA.

Base Sequence↗