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Biomedical subjects

Y Ise

Publications and source records attributed to Y Ise.

At least 19 recordsLinked to original sources
PubMed2004

Role of the calcium/calmodulin-dependent protein kinase ii (CaMKII) in the morphine-induced pharmacological effects in the mouse.

Calcium/calmodulin-dependent protein kinase II (CaMKII) is a family of multifunctional protein kinases that activates signaling pathways. The present study was designed to ascertain whether CaMKII could play a substantial role in the expression of morphine-induced antinociception, hyperlocomotion and rewarding effect in the mouse. An i.c.v. pretreatment with a CaMKII inhibitor KN-93 failed to affect the antinociception and hyperlocomotion induced by s.c. administration of a prototype micro-opioid receptor agonist morphine. In contrast, the morphine-induced place preference was significantly attenuated by i.c.v. pretreatment with KN-93. The levels of phosphorylated-CaMKII (p-CaMKII) in the limbic forebrain, but not in the frontal cortex and the lower midbrain, were significantly increased in morphine-conditioned mice, whereas the levels of CaMKII in three brain regions obtained from morphine-conditioned mice were not changed. This up-regulation of p-CaMKII in the limbic forebrain obtained from morphine-conditioned mice was significantly inhibited by i.c.v. pretreatment with KN-93. These results provide evidence that the increase in CaMKII activity in the mouse limbic forebrain may contribute to the rewarding effect, but not the antinociception and the hyperlocomotion, induced by morphine.

Animals↗
PubMed2001

Effects of fluvoxamine on morphine-induced inhibition of gastrointestinal transit, antinociception and hyperlocomotion in mice.

In the present study, the effects of fluvoxamine on the inhibition of gastrointestinal transit, antinociception and hyperlocomotion induced by morphine were investigated in mice. Treatment with morphine (10 mg/kg, subcutaneously (s.c.)) inhibited gastrointestinal transit and hyperlocomotion. These effects were attenuated by fluvoxamine in a dose-dependent manner. On the other hand, morphine (1 mg/kg, s.c.)-induced antinociception was significantly potentiated by fluvoxamine. These results suggest that fluvoxamine combined with morphine may be useful for the treatment of not only depression but also pain in cancer patients without constipation or activation of the mesolimbic dopaminergic system. However, further understanding and clinical studies will be required to confirm this possibility.

Analgesics, Opioid↗
PubMed2000

Modulation of opioidergic system on mecamylamine-precipitated nicotine-withdrawal aversion in rats.

RATIONALE: Several lines of evidence indicate that central opioid systems may be involved in the behavioral effects of nicotine. We previously reported that mecamylamine-precipitated nicotine-withdrawal aversion can be evaluated using the conditioned place preference paradigm. OBJECTIVES: In the present study, modulation of opioidergic systems in mecamylamine-precipitated nicotine-withdrawal aversion was investigated. METHODS: Male Sprague-Dawley rats were chronically treated s.c. with 10 mg/kg/day (-)-nicotine tartrate using an osmotic minipump. After nicotine treatment for 7 days, conditioning sessions were performed. In the morning, the rats were treated with mecamylamine (0.3-3.0 mg/kg, s.c.), hexamethonium (1.0-3.0 mg/kg, s.c.), naloxone (0.1-1.7 mg/kg), or saline (1.0 ml/kg, s.c.) in one compartment for 60 min. In the evening of the same day, rats were treated with the other treatments and confined to the other compartment for 60 min. Rats were treated with morphine (3.0 mg/kg, s.c.) or TAN-67 (56.0 mg/kg, s.c.) 30 min prior to mecamylamine injection in the conditioning session. On the next day of conditioning, tests were performed. RESULTS: Mecamylamine, which is known to pass the blood-brain barrier, produced a dose-dependent place aversion. However, hexamethonium, which fails to penetrate the blood-brain barrier, failed to produce a place aversion. Mecamylamine-precipitated nicotine-withdrawal aversion was significantly attenuated by pretreatment with the mu-opioid receptor agonist morphine and the highly selective delta-opioid receptor agonist TAN-67, which was administered 30 min before mecamylamine injection in the conditioning session. Moreover, naloxone at doses (0.1-1.7 mg/kg) that alone failed to show a place aversion in non-treated rats, produced a dose-dependent place aversion in rats that had been chronically treated with nicotine. CONCLUSIONS: These results suggest that central opioid systems may be involved in nicotine-withdrawal aversion.

Analgesics↗
PubMed1999

Mecamylamine-precipitated nicotine-withdrawal aversion in Lewis and Fischer 344 inbred rat strains.

Many studies have indicated that Lewis and Fischer 344 inbred rat strains show marked differences in behavioral responses to abused drugs. In the present study, we sought to determine whether these two strains of rats show different responses in mecamylamine-precipitated nicotine-withdrawal aversion using the conditioned place preference paradigm. Rats were treated subcutaneously with 10 mg/kg/day nicotine for 7 days using an osmotic minipump. After chronic nicotine infusion, the nicotinic receptor antagonist mecamylamine produced a significant place aversion in Lewis, but not in Fischer 344 rats. These results suggest that mecamylamine-precipitated nicotine-withdrawal aversion is strongly regulated by genetic factors.

Animals↗
PubMed1998

An autopsy case of cytokeratin 7-positive minute adenocarcinoma of the lung with systemic metastases.

We describe a 60-year-old woman with leg pain. Although metastatic bone tumor and atypical cells mimicking signet-ring cells in the bone marrow picture were observed, systemic survey revealed no primary lesion. The patient died two months after admission from systemic progress of the disease. Autopsy revealed a small focus of adenocarcinoma within the right upper lobe of the lung and systemic metastases without any particular changes in the gastrointestinal tract. The tumor cells of the lung were diffusely positive for cytokeratin 7, whereas cytokeratin 20 immunoreactivity was weak and focal, and that supported the lung origin of the present tumor. Moreover, the tumor cells in the bone marrow showed a similar pattern in immunoreactivity. These findings suggest that cytokeratin 7 and cytokeratin 20 immunoreactivity is helpful for the premortem diagnosis of the metastatic tumor of unknown origin.

Biomarkers, Tumor↗
PubMed1997

Attenuation of mecamylamine-precipitated nicotine-withdrawal aversion by the 5-HT3 receptor antagonist ondansetron.

The effect of ondansetron (0.01-0.1 mg/kg, s.c.), a selective 5-HT3 receptor antagonist, on mecamylamine-precipitated nicotine-withdrawal aversion was examined in the conditioned place preference paradigm. Male Sprague-Dawley rats were chronically treated subcutaneously with 9 mg/kg/day (-)-nicotine tartrate using an osmotic minipump. After nicotine treatment for 7 days, mecamylamine (1 mg/kg, s.c.), a nicotinic receptor antagonist, produced place aversion in nicotine-dependent rats. This aversive effect was dose-dependently antagonized by pretreatment with ondansetron 30 min prior to the conditioning. These results suggest that ondansetron may attenuate the place aversion associated with nicotine withdrawal, and may be useful for the treatment of nicotine dependence.

Animals↗
PubMed1996

Mecamylamine-precipitated nicotine-withdrawal aversion in rats.

The present study examined a rapid and convenient model for evaluating nicotine dependence using the conditioned place preference paradigm. Rats were chronically infused subcutaneously with 9 mg/kg per day nicotine using an osmotic minipump. After nicotine infusion for 7 days, the nicotinic receptor antagonist mecamylamine produced a place aversion in nicotine-dependent rats, but not in acute nicotine-treated rats or sham-operated rats. These results suggest that the mecamylamine-precipitated withdrawal aversion in rats chronically treated with nicotine may result from physical dependence on nicotine, and may be useful for studying the physical dependence on nicotine.

Analysis of Variance↗
PubMed1988

Immunological studies on the respiratory burst oxidase of pig blood neutrophils.

Recently, a flavin enzyme (pI 5.0), that is probably responsible for superoxide (O2-)-generated oxidase activity, was separated by isoelectric focusing-polyacrylamide gel electrophoresis (IEF-PAGE) from neutrophil membranes in our laboratory [(1987) J. Biol. Chem. 262, 12316-12322]. In the present work, we performed immunological studies on this enzyme derived from pig blood neutrophils. The enzyme extract obtained on IEF-PAGE was injected into guinea pigs to raise antibodies. IgG antibody against the pI 5.0 protein inhibited maximally 54% of the O2- -generating activity of the membrane-solubilized oxidase, whereas the normal serum IgG was not inhibitory at all. Our results further confirmed that the enzyme (PI 5.0) is one of the component(s) of the O2- -generating system. The enzyme gave rise to a band corresponding to a major protein of 72 +/- 4 kDa on both non-denaturing and SDS-PAGE. Immunoblotting after SDS-PAGE demonstrated labelling of peptides of 70-72, 28-32 and 16-18 kDa.

Animals↗
PubMed1988

Molecular mechanisms underlying lymphocyte recirculation. I. Functional, phenotypical and morphological characterization of high endothelial cells cultured in vitro.

Large-scale extravasation of lymphocytes takes place in vivo under physiological conditions in lymph nodes at very specialized vascular segments called high endothelial venules (HEV). When circulating lymphocytes leave the blood, they first bind to endothelial cells of HEV (HE cells) and subsequently enter lymph nodes by crossing the endothelial lining of HEV. Although the lymphocyte-HEV interaction has recently been the subject of intense research by many laboratories, it has been studied almost exclusively by the use of the lymphocyte-binding assay in which lymphocyte binding is examined on nonviable HEV present on frozen sections and, hence, no dynamic interaction between HE cells and lymphocytes has been studied. We report herein that endothelial cells of rat HEV can be grown in vitro and that the lymphocyte-HEV interaction can be studied dynamically using viable cells in culture vessels. The identification of the cultured line, termed Ax, as HE cells was based on their phenotypic, morphological, cytochemical and biochemical characteristics, and most importantly on its in vitro behavior, particularly in terms of its specific ability to interact with mature lymphocytes. Phenotypic analysis demonstrated that not only did monoclonal antibodies, known to react with HE cells, recognize the Ax but also a monoclonal antibody raised against the Ax specifically recognized HE cells in vivo, as determined by an immunoperoxidase staining of frozen sections, supporting the notion that the cell strain, Ax, is derived from HEV. This Ax, even after long-term culture (greater than 50 passages), allowed mature, but not immature, lymphocytes to bind to the cell surface and subsequently transport bound cells underneath their cytoplasm. This phenomenon was inhibited in a dose-dependent manner by various reagents known to inhibit lymphocyte recirculation in vivo. The cultured line derived from HE cells should provide a means to investigate the biochemical nature of lymphocyte-HEV interaction, and to understand the molecular mechanisms underlying the large-scale lymphocyte traffic taking place in vivo.

Animals↗
PubMed1987

Detection of toxoplasma IgM antibody by passive latex agglutination reaction.

Three major components (designated Sp-1, -2 and -3) of the microsomal pellet of Toxoplasma gondii (Tp) were isolated by Ultragel AcA 44 gel filtration chromatography from the microsomal pellet solubilized with detergents. Of these, Sp-2 proved to be most reactive with anti-Tp antibodies and its reactivity with IgM and IgG antibodies varied with the concentration at which it was used for sensitizing latex particles. Sp-2 antigen reacted with IgM antibody alone when latex particles were sensitized with less than or equal to 100 micrograms of this antigen/mg of particles, and its reactivity with IgG antibody appeared and increased progressively with increasing sensitizing concentrations of this antigen. Based on this finding, a method of direct measurement of anti-Tp IgM antibody in serum by passive latex agglutination has been developed. Polyacrylamide disc gel electrophoretic analysis of Sp-2 antigen in the presence of SDS revealed four constituents of 43, 35, 28 and 14 kDa. All these components reached with both IgM and IgG when tested by immunoblotting.

Animals↗
PubMed1987

Interaction of hepatitis B surface antigen with serum albumin of various species on polystylene latex particles.

Polystylene latex particles coated with serum albumin of various species, including non-primate serum albumin, were agglutinated by sera containing hepatitis B surface (HBs) antigen and hepatitis Be (HBe) antigen and by purified HBs antigen. Monomer and polymer albumin separated from human serum albumin preparations on latex particles were found to react with HBs antigen. Monomer from non-primate serum albumin preparations bound to latex particles was also found to have the ability to react with HBs antigen, but polymer of non-primate serum albumin did not. The mechanism of reaction between HBs antigen and the latex-bound serum albumin of various species is discussed.

Animals↗
PubMed1985

Detection of circulating antigens in sera of rabbits infected with Toxoplasma gondii.

Experiments were performed to investigate the usefulness of an enzyme-linked immunosorbent assay utilizing avidin-biotin interaction as a diagnostic tool for detection of Toxoplasma antigen in blood. The lower limit of sensitivity of the assay by this method was ca. 4 ng/ml, and standard assays provided a linear plot of antigen concentration over a range up to 250 ng/ml. In rabbits inoculated subcutaneously with trophozoites of the RH strain, Toxoplasma antigen became demonstrable in the circulation 3 days after injection, before emergence of antibody in serum and development of parasitemia. Analysis of the antigen in serum from infected rabbits by high-permeation liquid gel chromatography suggested the occurrence of antigens of four different molecular weights, greater than or equal to 400,000, 220,000, 130,000, and 45,000. Of these antigens, those of molecular weights 220,000 and 130,000 showed a conspicuous elevation with time after infection.

Animals↗