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Y K Su

Publications and source records attributed to Y K Su.

10 recordsLinked to original sources
PubMed1994

[Quantitative analysis of the cellular DNA and RNA contents of hydatidiform mole and its clinical significance].

The cellular DNA and RNA contents of 152 patients with hydatidiform moles (69 benign and 83 malignant) were analysed by flow cytometry (FCM), and strict follow-up was implemented. The chorionic villi of twelve cases in early pregnancy were used as normal control and another 12 cases of choriocarcinoma as positive control. Retrospective as well as prospective studies were conducted. The results showed that the accuracy rates of diagnosis of DNA aneuploid and RNA were respectively 66.7% and 82.6% in benign moles, 68.7% and 81.9% in invasive moles. Statistically the RNA parameter was significantly more sensitive the DNA parameter (P < 0.05). Analysis with both RNA and DNA parameters in predicting malignancy, the accuracy rate was 94.0%. Our study suggests that both the aneuploidy DNA and RNA contents may be important indicators for forecasting malignant change. The RNA parameter is more sensitive than the DNA parameter, and the use of both parameters is more accurate than either one alone. In addition, the concept of "standard DNA index" is raised.

Aneuploidy↗
PubMed1993

Flow cytometric analysis of cellular DNA and RNA contents of hydatidiform mole. Two significant parameters indicating malignant nature and prognosis.

The cellular DNA and RNA contents of trophoblastic tissue of 86 patients with trophoblastic disease and chorionic tissue of 10 patients in early pregnancy were analysed by flow cytometry (FCM) and strict follow-up was carried out. The result showed the rate of accurate diagnosis by DNA and RNA determinations were 60.0% and 85.7% respectively in benign moles; 86.7% and 86.7% in invasive moles; and 100% and 100% in choriocarcinoma. Statistically the pseudopositive percentage of RNA was clearly lower than that of DNA (0.01 < P < 0.05). In addition, the trophoblastic cells in curetted molar tissue and the uterine tissue later proved by surgery of 10 invasive moles were simultaneously assayed for comparing their DNA and RNA contents, showing statistical significance (P < 0.001). The DNA and RNA contents in different cellular cycles were analysed by FCM and there was no statistical significance among benign, malignant moles and choriocarcinoma (P > 0.05). Our study suggests that aneuploidy DNA content may be an indicator for malignant tendency and the RNA parameter rather than DNA is more sensitive for the prognosis of hydatidiform mole.

Adult↗
PubMed1992

Delivery by direct intrauterine transfer of gametes.

A nurse, 27, primary sterility for 5 years, had normal menstruation, normal size of uterus anterior. HSG: Rt. tube was not visible, Lt. tube slightly enlarged at ampulla. Ovaries were over-stimulated by clomiphene, HMG and hCG.8 ova were taken through vaginal aspiration under B-mode ultrasound scanning. Direct introduction of ova and washed sperms simultaneously into the uterus on Sept. 19, 1991 resulted in successful single pregnancy up to 36(+1) weeks. A 2 250 g normal baby boy was delivered on May 15, 1992 spontaneously. The base and prospect of this manoeuver are discussed with a reference to Craft's procedure.

Adult↗
PubMed1992

[Delivered by direct intrauterine transfer of gametes].

A nurse, 27, primary sterility for 5 years with normal pelvic findings. Hysterosalpingogram showed right tube not visualized, and impatent, left tube with slight enlargement at ampulla. Her last menstrual period was September 5, 1991. Ovaries hyperstimulated by clomiphene, HMG and hCG, and 8 ova taken through vaginal aspiration under B scanning on September 19, 1991. Ova and washed sperms were directly introduced into uterine cavity simultaneously. It turned out to be a successful clinical pregnancy, carried on smoothly up to 36(+1) weeks. She spontaneously delivered a healthy male infant weight 2250 grams on May 15, 1992.

Adult↗
PubMed1991

[Embolus formation using bismuth polyurethane for tubosterilization observation of 259 cases].

A 50% solution of bismuth polyurethane in absolute alcohol, which remains in a liquid state at 70 degrees C and solidifies at body temperature within a few minutes, was used to form embolus in the fallopian tubes for the purpose of tube-sterilization. This compound is non-caustic and proves to be bioadaptable. A modified Foley's catheter was used to introduce the solution into the lumens of the tube by way of the uterine cavity in 259 cases. A 24 month follow-up by life table method was carried out, and 26 were pregnant with a pregnancy rate of 10.0%. The number of successful operation at the first attempt was 234 with 18 cases pregnant (7.7%). The recanalization rate of the tubes within 24 months was 11.4% including those of pregnancy. There was little side reactions postoperatively. A polyethylene 2 way tube with a rubber bulb for instillation of the bismuth polyurethane had been made on October 1990. Ways of improving the successful rate were mentioned. The authors believe that this is a useful procedure of tubo-sterilization, which is easily accepted by the patients and easily carried out by the medical personnel.

Bismuth↗
PubMed1989

[Prediction of the malignant tendency of hydatidiform mole by determination of DNA content].

The cellular DNA content of paraffin-embedded molar tissue blocks obtained from the first curettage was measured by flow cytometry in 64 cases of hydatidiform mole. Of these 64 cases 32 developed malignant changes and 32 remained persistently benign during a period of follow-up of 0.5 to 7.5 years. The proportion of aneuploid was 59.4% in patients demonstrating malignant changes and 34.4% in those with persistent benign diseases. The difference was statistically significant (P less than 0.05). This preliminary result suggests that DNA aneuploid may be used as a parameter for the prediction of possible malignant changes and selecting of indications for prophylactic chemotherapy.

Aneuploidy↗
PubMed1983

Coding assignments of each mRNA synthesized in vitro and protein components of CPV of silkworm Bombyx mori.

The coding properties of each mRNA synthesized in vitro of CPV have been examined. The mRNAs were separated by polyacrylamide gel electrophoresis and isolated from the gel slices. The individual mRNA was then translated into the wheat germ cell-free protein synthesizing system. The products of translation were analyzed by the immuno-reaction with the 125I-IgG of each of the five electrophoretic protein components of CPV. The coding assignments were deduced on the basis of the immuno-reaction as follows: mRNA1, mRNA3 and mRNA9 encode group P1 of viral proteins, mRNA2 and mRNA6 encode group P2 and P3, respectively. mRNA7 and mRNA8 encode group P4 while mRNA9 encode group P5. There are more than 10 polypeptides in the CPV virion as demonstrated by two-dimensional electrophoresis.

Animals↗