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Biomedical subjects

Y Kaneshige

Publications and source records attributed to Y Kaneshige.

13 recordsLinked to original sources
PubMed2005

Effect of the removal of cumulus cells on the nuclear maturation, fertilization and development of porcine oocytes.

The present study was conducted to investigate the effects of attachment of cumulus cells to porcine oocytes during the process of maturation and fertilization on the nuclear maturation, fertilization and subsequent development after in vitro fertilization (IVF). In the first experiment, the cumulus cells were removed from cumulus-oocyte complexes (COCs) at 0, 24 and 42 h after the onset of maturation culture and were then cultured until reaching 42 h of cultivation. In the second experiment, COCs were denuded as described in the first experiment, then fertilized and cultured for 7 days. As a control, cumulus cells were allowed to maintain attachment to the oocytes until the end of IVF. The proportion of oocytes reaching metaphase II significantly increased with the delay in the removal treatment of cumulus cells. The proportion of normal fertilization gradually increased with delay in the removal treatment of cumulus cells from COCs until the end of IVF. However, no significant difference in the proportion of normal fertilization was found between the 42-h and control groups. The removal treatment of cumulus cells in the 0- and 24-h group significantly (p < 0.05) decreased the proportion of cleaved embryos when compared with the control, and none of them developed to the blastocyst stage. The proportion of development to the blastocyst stage was significantly higher (p < 0.05) in the control group than in the 42-h group (18.1% vs 12.4%; p < 0.05). The present study indicates that the attachment of cumulus cells to the oocyte during maturation and fertilization is important to support oocyte nuclear maturation, fertilization and subsequent embryo development. Particularly, the attachment of cumulus cells to the oocyte during IVF promotes embryonic development.

Animals↗
PubMed1992

A correlation study between blink reflex habituation and clinical state in patients with Parkinson's disease.

The neurologic states and activities of daily life of patients with Parkinson's disease were evaluated using a rating scale with subitems, and subsequently the neurologic disturbance scores and the daily activity impairment scores were obtained. Subjects consisted of 19 normal controls, and 55 ambulatory patients without marked dyskinesia who were on various anti-parkinsonian drugs. Blink reflex was elicited by paired electrical stimulation over the supraorbital nerve. The interval time between the conditioning stimulation and the test stimulation was set at 200 ms, and 5 serial ipsilateral maximal R2 amplitudes on the stimulated side were measured. The mean of the paired maximal R2 amplitude ratio (test/conditioning), expressed as a percentage, was defined as the habituation index. The habituation indices in normal controls and those with Parkinson's disease were 17.1 +/- 7.6 and 51.9 +/- 29.3, respectively (P less than 0.01). The degree of akinesia, rigidity, balance/gait and dysarthria was positively correlated with the habituation index (P less than 0.01), while tremor was not. On the whole the habituation index was found to have a significant correlation not only with the neurologic disturbance score but also with the daily activity impairment score (P less than 0.01).

Activities of Daily Living↗
PubMed1991

[A case of Guillain-Barré syndrome associated with cytomegalovirus infection].

A 23-year-old man was admitted to our hospital because of numbness in toes and finger tips. Within the next 72 hours general muscular weakness progressed so rapidly that he could not walk and necessitated a respirator. From the 18th hospital day plasmapheresis resumed 4 times every other day, which was followed by methylprednisolone pulse therapy (1,000 mg/day) for 3 successive days. The combination treatment described above brought about a dramatic recovery. Throughout the entire clinical course, enzyme immunoassay (serum) and enzyme-linked immunosorbent assay (CSF) to cytomegalovirus (CMV) were carried out serially. These results supported that he developed Guillain-Barré syndrome associated with CMV infection. Electrophysiological studies of the median and ulnar nerves were also performed serially up to 6 months after the onset. Based on the results the following comments were made; (1) In acute phase the amplitude markedly reduced with relative preservation of the distal latency, suggesting the presence of conduction block and mild demyelination. (2) Plasmapheresis, if done early enough, could prevent forthcoming secondary axonal degeneration probably by removing unknown nerve conduction blocking agents. (3) The degree of the distal latency prolongation at early phase was not a useful indicator for predicting the prognosis.

Adult↗
PubMed1990

Correlation of somatosensory evoked potentials and long loop reflexes in patients with multiple sclerosis.

Based on the results of somatosensory evoked potentials (SEPs) and long loop reflexes (LLRs) obtained from the 50 upper limbs of 25 normal controls by stimulating the median nerve, four regions were set up in a latency correlation diagram between N20 and LLR, which were assumed to mainly represent the afferent and the efferent functions, respectively. Seventy upper limbs of patients with multiple sclerosis, differently marked according to the presence or absence of pyramidal sign and/or impaired vibration sense, were plotted on the diagram. The regions to which the patient belonged turned out to be reasonably compatible with the neurological status of the patient. Simultaneous measurements of SEPs and LLRs are therefore useful to evaluate the afferent and efferent pathways in the central nervous system.

Adult↗
PubMed1990

[Age-related interrelation of vibration perception thresholds and somatosensory evoked potentials in normal subjects].

In order to establish the age-related changes of vibration perception thresholds (VPTs) and somatosensory evoked potentials (SEPs), which are known to share a common afferent pathway in the central nervous system, 119 normal subjects ranging in age from the 20s to the 90s were studied. The VPTs of the index fingertip were measured at 63 Hz, 125 Hz and 250 Hz by a Rion vibrometer, nd the latencies of the SEPs to the median nerve stimulation (N9, N13 and N20) were simultaneously evaluated. The VPT, tended to elevate from the 40s, became increased towards higher frequencies (63 Hz less than 125 HZ less than 250 Hz) in the 80s to the 90s, whereas the VPT in younger ages showed just an opposite pattern (63 Hz greater than 125 Hz greater than 250 Hz). None of the height-corrected latencies of N9, N13, N20 and central conduction time (N20-N13) revealed a significant correlation between the VPTs at any frequencies. The age-related decline of vibration sense, therefore, could not be explained solely by the degenerative changes of he posterior column and/or the delay of the peripheral nerve conduction velocity. This phenomenon was rather thought to be compatible with an age-associated characteristics of Pacinian corpuscles.

Adolescent↗
PubMed1989

[Evaluation of digital vibration threshold for asymptomatic ulnar neuropathy in patients with carpal tunnel syndrome].

Quantitative assessment of vibration threshold at 125 Hz of index and little finger tips together with measurements of median and ulnar sensory nerve conduction velocity (SNCV) was performed for 25 limbs of 15 patients with carpal tunnel syndrome (CTS), 25 limbs of 13 disease controls with neurological symptoms similar to CTS and the nearly same number of limbs of normal controls. There was no significant difference in ulnar SNCV among 3 groups. However, ulnar SNCV at wrist was significantly reduced in patients with CTS as compared with normal value. In 17 limbs with CTS, vibration threshold of index finger tips was significantly increased, and 8 of those limbs were found to have concomitant increase of vibration threshold of little finger tips. Most of patients with increase of vibration threshold of both index and little finger tips were those who used to expose to repetitive mechanical stimuli at the wrist in their occupation. It was concluded that digital vibration threshold is simple to measure yet sensitive enough to detect asymptomatic ulnar neuropathy in CTS.

Adult↗
PubMed1989

[Correlation between somatosensory evoked potentials and long loop reflexes--basic investigation and its application for multiple sclerosis].

Somatosensory evoked potentials (SEPs) and long loop reflexes (LLRs) to the median nerve stimulation were investigated on 25 normal controls and 25 patients with multiple sclerosis (MS). Fifteen normal controls were also subjected to LLR study by the common peroneal nerve stimulation. The mean height were 159 +/- 8.2 cm in normal controls and 160 +/- 8.9 cm in MS, respectively. LLRs were obtained with 100% reproducibility in all cases. Upper limb LLRs were recorded from m. abductor pollicis brevis by trigger stimulation during isotonic contraction of the thumb, while lower limb LLRs were recorded from m. peroneus longus by trigger stimulation during isotonic eversion of the foot. The threshold of LLR was lower than that of short latency reflex (H-wave) with the mean latency of 40.4 +/- 1.5 ms. The height of subjects revealed an obvious positive correlation not only with the latency of LLR but also with N 20 of SEP, whereas central conduction time was not. Furthermore, a significant correlation was present between the latencies of LLR and N 20, showing a twofold gradient of LLR against N20. There was a significantly prolonged latency difference between H-wave and LLR of lower limb as compared with that of upper limb. When the stimulation site was changed from the wrist to the elbow, the latency difference between M-wave and H-wave shortened. This fact, therefore, appears to be against "resonance hypothesis" that LLR is set off according to the intrinsic mechanical oscillation given to the muscle concerned.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1989

[A case presenting manifestations of bulbospinal muscular atrophy with senile onset, rapid progression and marked asymmetry].

A 64-year-old man was admitted to our department because of muscle cramp, atrophy and weakness of the limbs together with difficulty in walking, which had gradually progressed from age 60. About 1 year prior to admission, he had noticed hand tremor and gynecomastia. On admission, neurological examination revealed diffuse muscle atrophy and weakness of the extremities, which were more obvious on the right side with preponderance in the right leg. Bilateral postural hand tremor was also more prominent on the right hand. Fasciculations were observed both in the extremities and tongue. The remaining cranial nerves and cerebellar functions were intact. Sensation was normal except for slightly decreased vibratory sense in the distal part of the legs. Deep tendon reflexes including jaw jerk were increased with the exception of hyporeflexia of the right leg. Babinski sign was negative bilaterally. Blood examination disclosed slight elevation of CK and fasting glucose level of 110 mg/dl. Glucose tolerance test showed a diabetic pattern. CSF examination showed total protein of 74 mg/dl and IgG of 12 mg/dl. On a series of endocrinological studies, there was no significant elevation of androgen and estrogen both in serum and urine except for slight elevation of serum E1 level. Serum LH and FSH, however, were markedly high, which responded far beyond the normal range following to 0.1 mg injection of LH-RH. These results suggested that gynecomastia might be caused by dysfunction of the hypothalamus-hypophysis system. Brain CT and spine MRI showed no abnormality. Muscle biopsy obtained from the right quadriceps femoris revealed neurogenic abnormalities.(ABSTRACT TRUNCATED AT 250 WORDS)

Age Factors↗
PubMed1989

[Determination of CSF guanase activity in multiple sclerosis: a comparative study on various parameters to monitor the disease activity].

Forty two patients with multiple sclerosis (MS) were subjected to determination of CSF guanase activity, CSF IgG concentration, Tibbling's IgG index and Kurtzke's expanded disability status scale (EDSS) together with multimodal evoked potentials (MEPs), which consisted of somatosensory evoked potentials to arm and leg stimulation, visual evoked potential and auditory brainstem response. All patients were divided into active (n = 28) and inactive (n = 14) group, where an "active" was defined as a case revealing a relapse within one month prior to the study. The results were as follows: 1. CSF guanase activity was significantly high in active MS as compared with inactive MS (p less than 0.001), and the same trend was found in CSF IgG concentration (p less than 0.01), but not in IgG index. 2. Abnormal MEP was closely associated with elevated CSF guanase activity in active MS (p less than 0.05), but not in inactive MS. 3. In regard to the disease activity, EDSS was significantly high in active MS as compared with inactive MS (p less than 0.01), and EDSS was also significantly high in abnormal MEP group (p less than 0.001). 4. EDSS was closely correlated with CSF guanase activity in active MS (p less than 0.005), but not in inactive MS. 5. Judging from serial determinations of the parameters in 6 patients, CSF guanase activity, less susceptive to steroid hormone therapy than CSF immunoglobulin level, was found parallel to EDSS, however, both CSF IgG concentration and IgG index failed to correlated with EDSS. In summary, CSF guanase activity was thought to be a sensitive parameter in multiple sclerosis, reflecting not only the disease activity but also spatial involvement in the CNS.

Adult↗