Clinical effects and bronchoalveolar transfer of levofloxacin in patients with community-acquired pneumonia.
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Adult↗
Biomedical subjects
Y Lin
Publications and source records attributed to Y Lin.
Detection of human herpesvirus 8 DNA in acute leukemia patients.
OBJECTIVE: To determine the prevalence of human herpesvirus 8 (HHV-8) DNA in acute leukemia (AL) patients. METHODS: The presence of HHV-8 DNA sequences in peripheral blood mononuclear cells (PBMC) and bone marrow mononuclear cells (BMMC) from 50 AL patients was examined using polymerase chain reaction (PCR). Nine human hematopoietic cell lines and PBMC from 30 normal donors were also included. RESULTS: HHV-8 DNA sequences were detected in one case of acute myelogenous leukemia (AML). The specimens from the bone marrow aspirate, peripheral blood and serum of this patient were all positive. None of the normal donors and human hematopoietic cell lines showed evidence of HHV-8 DNA. CONCLUSION: The results suggest that the prevalence of HHV-8 is low in AL in China.
Acute Disease↗
Analysis of the T cell receptor V delta region gene repertoire in bronchoalveolar lavage fluid (BALF) and peripheral blood of asthmatics.
OBJECTIVE: To explore the role of gamma delta T cells in the airway of asthmatics and to identify the forces which induce and maintain the inflammatory process. METHODS: Peripheral blood (PB) and bronchoalveolar lavage fluid (BALF) were obtained from 7 asthmatic subjects and 7 nonsmoker control subjects. The percentage of gamma delta T cells in the PB and BALF was measured by immunofluorescent staining and flow cytometry. The frequency of usage and the clonality of V delta subfamilies (V delta 1-V delta 3) were assessed by RT-PCR and gene scanning. RESULTS: A higher proportion of gamma delta T cell was detected in the BALF of asthmatic subjects (7.8% +/- 4.7%) than that from control subjects (3.3% +/- 3.0%, P = 0.04). No selective usage for a particular V delta subfamily was found, but the relative expression level of V delta 1 was significantly higher in the asthmatic airway (44% +/- 13%) than in the control (19% +/- 5%, P = 0.0002). In asthmatic subjects, the monoclonal or oligoclonal expansion of gamma delta T lymphocytes was predominant in the BALF, especially V delta 1+ T lymphocytes. CONCLUSIONS: Antigenic specific gamma delta T cells might play an important role in the inducement and maintenance of airway inflammation. Persistent antigenic stimulation may be the key factor that maintains chronic airway inflammation in asthma.
Adult↗
Role of beta-adrenoceptor at different stages of bronchial asthma.
OBJECTIVE: To investigate the changes of beta-adrenergic receptor (beta AR) in peripheral lymphocytes and beta 2AR mRNA levels at different stages of bronchial asthma. METHODS: beta 2AR density and beta 2AR mRNA level in peripheral lymphocytes, cAMP and cGMP levels in blood plasma were estimated by radioligand binding assay, radioimmunoassay and RT-PCR. RESULTS: (1) Maximum bound volume (Bmax) and equilibrium dissociation constant (Kd) of beta 2AR of lymphocyte in asthma patients at remission stage were markedly higher than that in normal subjects, while cAMP levels in blood plasma showed no difference. Bmax of beta 2AR and cAMP levels in asthma patients at acute exacerbation stage were significantly lower than that in normal subjects, and Kds between these two groups were not much different. (2) Expression of beta 2ARmRNA in peripheral lymphocytes of asthmatics at remission stage was not significantly different compared with that in normals. CONCLUSIONS: Amount and function of beta AR and beta 2ARmRNA levels are related to asthmatic conditions. Changes of beta AR and beta 2ARmRNA in asthma might rather be a pathological change accompanied by the course of the disease than a primary defect.
Adult↗
[Analysis of the T cell receptor Vdelta region gene repertoire in asthmatic subjects].
OBJECTIVE: To explore the role of gammadelta T cells in the asthmatic airway inflammation and identify the forces which induce and maintain the inflammatory process. METHODS: Peripheral blood (PB) and bronchoalveolar lavage fluid (BALF) were obtained from seven asthmatic subjects and seven nonsmoking control subjects. The percentage of gammadelta T cells in PB and BALF were measured by immunofluorescent staining and flow cytometry, the frequency of usage and the clonality of Vdelta subfamilies (Vdelta(1) approximately Vdelta(3)) were assessed by RT-PCR and gene scanning. RESULTS: Higher proportion of gammadelta T cell was detected in the BALF of asthmatic subjects [(7.8 +/- 4.7)%] than that from control subjects [(3.3 +/- 3.0)%] (P < 0.05) and the relative expression level of Vdelta(1) significantly higher in the asthmatic airway [(44 +/- 13)%] than in the control group [(19 +/- 5)%] (P = 0.002). In asthmatic subjects, the monoclonal or oligoclonal expansion of gammadelta T lymphocytes was predominant in BALF, especially Vdelta(1) T lymphocytes. CONCLUSIONS: Antigenic specific gammadelta T cells might play important roles in the inducement and maintenance of airway inflammation.
Adult↗
[The role of neutrophils and interleukin-8 in pathogenesis of asthma].
OBJECTIVE: To investigate the role of neutrophils and interleukin-8 (IL-8) in pathogenesis of asthma. METHODS: Pulmonary function and bronchial provocation test were performed to identify and evaluate asthma. Superoxide anion O(2)(-.) generation of peripheral blood neutrophils, IL-8 and malondialhyde (MDA) levels in plasma and induced sputum were measured. RESULTS: The O(2)(-.) generation [(20.9 +/- 5.1) nmol] of peripheral blood neutrophils from moderate-severe asthmatics were significantly higher than that [(15.2 +/- 4.2) nmol] from mild asthmatics (P < 0.01), in which that was still significantly higher than that [(11.3 +/- 2.4) nmol] from normal subjects (P < 0.05). The O(2)(-.) generation of peripheral blood neutrophils had significantly negative correlation with PD(20)FEV(1) (r = -0.693, P < 0.05). The levels of IL-8 [(585 +/- 75) ng/L, (791 +/- 103) ng/L] and MDA levels [(6.3 +/- 1.6) mmol/L, (21.8 +/- 6.3) mmol/L] in plasma and induced sputum from unstable asthmatics were higher than those [(227 +/- 54) ng/L, (322 +/- 95) ng/L and (5.4 +/- 1.0) mmol/L, (15.1 +/- 5.6) mmol/L] from stable asthmatics (P < 0.01), more over, in the latter group, they were still significantly higher than those (188 +/- 46) ng/L, (224 +/- 51) ng/L and [(4.1 +/- 0.4) mmol/L, (9.5 +/- 4.2) mmol/L] from normal subjects (P < 0.05). The levels of MDA in the induced sputum from stable asthmatics showed a negative correlation with PD(20)FEV(1) (r = -0.708, P < 0.01). The levels of IL-8 in the induced sputum showed a positive correlation with percentage of neutrophils in the induced sputum (r = 0.838, P < 0.01). CONCLUSIONS: IL-8 and oxygen free radicals generated by neutrophils might participate in development of airway inflammation and airway hyperresponsiveness in asthma.
Adult↗
[Clinical and radiographic evaluation of 108 dental implants after 1-year loading].
OBJECTIVE: To evaluate the clinical outcomes and a possible correlation between peri-implant mucosal inflammation and marginal bone loss in a group of Chinese patients who received IMZ and Frialit-2 implants one year after restoration. METHODS: The clinical and radiography parameters of 108 IMZ and Frialit-2 dental implants in 70 patients were measured. RESULTS: There were no signs of mobility and peri-implant radiolucencies in any of the implants. The average bone loss adjacent to implants was (0.63 +/- 0.78) mm. The prevalence of peri-implant mucosal inflammation, as indicated by suclus bleeding index (SBI) of 1.0 or more, was 32.9%. Significant correlation was found between mucosal inflammation and bone loss. The severely inflamed sites experienced greater bone loss than that of healthy or slightly inflamed sites (P < 0.05). In 92% of sites, plaque index (PLI) of more than 1 was measured on implant surfaces. The periodontal status of the remaining natural teeth in each subject was evaluated using the community periodontal index of treatment need (CPITN). Of the 70 examined patients, 15 patients needed oral hygiene instruction, 44 needed scaling and root planing, 8 patients needed periodontal surgical therapy, and only 3 did not need any periodontal treatment. CONCLUSIONS: The 1-year outcomes from this patient series indicated that IMZ and Frialit-2 implant provide satisfactory clinical results. One of the etiologic factors of alveolar bone loss around implants seems to be plaque-associated marginal inflammation. Improvement of oral hygiene, elimination of marginal tissue inflammation, and proper treatment of periodontal disease of natural teeth are needed for maintaining the implants in these observed subjects.
Adolescent↗
[Studies on epidemic feature of enteric adenoviruses infection in feces of infants with diarrhea in Shenzhen].
OBJECTIVE: To estimate the enteric adenoviruses infection in feces of infants with diarrhea in Shenzhen. METHODS: All 195 fecal samples of infants were tested by PCR. Amplimers were analysed by Rsa I endonucleases and the type of enteric adenovirus identified. RESULTS: The positive rate of adenovirus DNA in fecal samples was 13.33% (26/195) while the prevalence rates of enteric adenovirus type 40, adenovirus type 41 and the other type of adenovirus were 2.56% (5/195), 8.21% (16/195) and 2.56% (5/195) respectively. Fever was the characteristic symptom of adenovirus infection. CONCLUSION: Enteric adenovirus type 40 and 41 were important pathogens which caused infant diarrhea in Shenzhen, with latter the majorty.
Adenoviridae Infections↗
[GATA-2 gene expression in leukemia patients and its significance].
OBJECTIVE: To investigate GATA-2 gene expression in leukemia patients and its clinical significance. METHODS: GATA-2 gene transcripts were detected by reverse transcriptase polymerase chain reaction (RT-PCR), and bcr/abl or PML/RAR alpha transcripts were detected in patients with chronic myeloid leukemia (CML) and acute promyelocytic leukemia (APL), respectively. RESULTS: The GATA-2 transcripts were revealed in 93% of acute myeloid leukemia (AML), in 70% of acute lymphoblastic leukemia (ALL), and 83% of CML patients, but not in normal bone marrow and peripheral blood cells. GATA-2 expression in remission patients was similar to newly diagnosed or relapsed patients. In post-transplantation patients, GATA-2 expression was significantly decreased. In these cases GATA-2 transcripts could be detected with bcr/abl fusion gene disappeared in 2 of 12 CML, whereas neither GATA-2 nor PML/RARalpha transcripts could be detected in APL. CONCLUSION: GATA-2 transcripts was highly expressed in leukemia patients and had no change in remission but significantly decreased in post-transplantation patients. Detection of GATA-2 expression would give information about the residual leukemia stem cells.
Bone Marrow Cells↗
The affects of acupuncture at sibai and neiting acupoints on gastric peristalsis.
The frequency and amplitude of gastric peristaltic wave were significantly changed in 15 subjects when Sibai (ST 2) and Neiting (ST 44) acupoints of the Stomach Channel of Foot-Yangming were punctured. The results demonstrate that these acupoints are effective for treatment of stomach diseases, providing an experimental basis for the theory of channels and collaterals.
Acupuncture Points↗
[IL-2 gene and cisplatin combined therapy for head and neck squamous cell carcinoma].
OBJECTIVE: The objective of this study is to assess the efficacy of IL-2 gene and cisplatin combined therapy on murine HNSCC models. METHODS: HNSCC tumors were established in murine models. The sizes of tumors were measured before and after combined IL-2 and cisplatin treatment, and the results were evaluated by comparing different single treatment groups and the control IL-2 expression was measured after IL-2 gene transfer. Cytotoxic T-lymphocyte (CTL) and natural killer (NK) assays were also performed. RESULTS: HNSCC tumor growth was significantly inhibited following combined IL-2 gene and cisplatin therapy, when compared with that of the control. The levels of IL-2 protein expressions were increased in combined and single IL-2 treated groups. The activities of cytotoxic T-lymphocytes and natural killers in both the combined treatment group and the IL-2 treated group are higher than those of the controlled group. CONCLUSION: Combined IL-2 gene and cisplatin therapy can significantly inhibit HNSCC tumor growth in the murine models, and efficiently induces anti-tumor immune reaction of hosts.
Animals↗
[Influence of the hearing loss level and auditory sensation curve type on click evoked SP/AP amplitude ratio in Menière's disease].
OBJECTIVE: To assess the influence of hearing loss level and auditory sensation curve type on click evoked SP/AP amplitude ratio (negative summating potential/action potential) in the diagnosis of Meniere's disease. METHOD: AP and SP was recorded with tympanic ECochG in normal controls (50 ears), patients with sensorineural hearing loss (SNHL) and patients with Meniere's disease (90 ears) which were further divided based upon the degree of hearing loss and audiometric configuration. SP/AP amplitude ratio was calculated and analyzed. RESULT: AP latency in the three groups had no significant differences. AP amplitude in normal hearing subjects was higher than other two patients groups (P < 0.05). Amplitude of SP of control group was higher than that of SNHL group (P < 0.01), but lower than Meniere's disease group (P < 0.01). SP/AP amplitude ratio in Meniere's disease ears was (48.09 +/- 14.83)%, which was significantly higher than that of SNHL group (20.20 +/- 15.00)% (P < 0.01) and normal group (23.85 +/- 8.05)% (P < 0.01). In Meniere's disease ears, SP/AP amplitude ratio was positively correlated to pure tone thresholds (r = 0.5601 P < 0.01). An elevated SP/AP ratio was found in 73% of the Meniere's ears and 6% of the SNHL ears. SP/AP ratio among the different levels of hearing loss in Meniere's disease ears, of the ears with mild hearing loss was lower than those with moderate (P < 0.01) and severe hearing loss (P < 0.01), but between later groups there was no statistical significant difference (P > 0.05). Positive rate of SP/AP ratio was 63% in mild, 83% in moderate, 73% in severe hearing loss ears respectively. SP/AP amplitude ratio in patients with low tone auditory curve type was lower than that with flat type(P < 0.05), the latter had no significant difference (P > 0.05) compared with high tone type. Positive rate was 67.57% in low tone type, 75% in high tone type and 78% in flat tone type respectively. CONCLUSION: Results suggests the increase of SP/AP ratio was of clinical significance in the diagnosis of Meniere's disease, and was with advancing pure tone thresholds in certain range of hearing loss levels. Click evoked SP/AP amplitude ratio was affected by degree of hearing loss and auditory sensation curve as well, perhaps the influence of the former on SP/AP ratio was considerable significant.
Action Potentials↗
[Determination of inorganic anions in beer and brewing water by ion chromatography].
An ion chromatography method was used to determine fluoride, chloride, nitrite, dihydrogen phosphate, nitrate and sulfate anions in beer and brewing water on a YSA8 8022A-9(250 mm x 4 mm i.d.) separation column, with sodium carbonate-sodium bicarbonate buffer as mobile phase and an electrical conductivity cell as detector. Qualitative analysis was performed with retention time and stanard spiking method, and quantitative analysis was conducted with a calibration curve by measuring the peak height. This method is simple, rapid and accurate.
Anions↗
[The study on effects of rhEGF on proliferation of human conjunctival epithelial cells].
PURPOSE: To investigate the effects of Recombinant Human Epidermal Growth Factor (rhEGF) on proliferation of human conjunctival epithelial cells and optimal dose in vitro. METHODS: Cultured human conjunctival epithelial cells were plated in 96-well plates at a density of 2,000 cells per chamber, treated with different concentrations of rhEGF (100 micrograms/ml, 50 micrograms/ml, 20 micrograms/ml, 10 micrograms/ml, 5 micrograms/ml and 1 microgram/ml). After 24, 48, 72 and 96 hours of treatment, cell viability was assessed with MTT assay. RESULTS: There was no significant difference in all experimental groups and the control group in the first 24 horus. Experimental groups at a concentration of 50 micrograms/ml, 20 micrograms/ml and 10 micrograms/ml had significantly higher cell viability than which of the control group 48 hours later. All groups of rhEGF had significantly higher cell viability than which of the control group after 72 hours treatment, except for 100 micrograms/ml of rhEGF. Each group of rhEGF after 96 hours treatment had higher cell viability than which of the control group, and 100 micrograms/ml of rhEGF had lower cell viability than which of the other experimental groups, with significant difference. CONCLUSION: rhEGF enhance proliferation of human conjunctival epithelial cells effectively in vitro. The optimal concentration ranged from 10 micrograms/ml to 50 micrograms/ml.
Cell Division↗
[Construction of recombinant plasmids containing genes of HBsAg and their expression in the eukaryotic cells].
OBJECTIVE: To construct recombinant plasmids expressing L, M, S and pre-S1-S protein of HBsAg. METHODS: Amplifying segments of S, pre-S2-S, pre-S1-pre-S2-S genes of HBV by PCR and amplifying segment of pre-S1-S by overlap extension PCR; inserting the segments into Rc/CMV and pSG5UTPL/Flag plasmids respectively and exploring their expressions by Western-Blot hybridization, identifying the inserting segments by sequencing. RESULTS: The sequences of the inserted segments were the same as the genes of S, pre-S2-S, pre-S1-pre-S2-S and pre-S1-S and the results of Western-Blot hybridization were positive for the aimed proteins. CONCLUSION: We have gained 8 recombinant plasmids expressing S, M, L and pre-S1-S proteins with high efficacy.
Base Sequence↗
[The protein expression of p53 and bcl-2 in laryngeal squamous cell carcinoma].
OBJECTIVE: To detect the relationship of p53 and bcl-2 gene to laryngeal carcinoma. METHODS: p53 and bcl-2 protein expressions were examined in the specimens of 60 laryngeal carcinomas and 8 vocal cord polyps by immunohistochemistry method. RESULTS: Overexpressions of p53 and bcl-2 were found in the laryngeal carcinoma specimens; the positive rates of p53 and bcl-2 protein expression were 61.7%(37/60) and 43.3%(26/60), respectively. Negative results were observed in all the vocal cord polyp specimens. The positive rates of p53 and bcl-2 expression were related to the histological grade and cervical lymphatic metastasis of laryngeal carcinoma but not related to clinical stage and tumor types. CONCLUSION: The results of this study support the hypothesis that p53 and bcl-2 gene may be involved in the apoptosis of laryngeal carcinoma and they may play an important role in the pathogenesis of laryngeal cancer.
Adult↗
[The effects of adenosine, interleukin-1 and theophylline on the expression of A2 adenosine receptor mRNAs in peripheral blood mononuclear cells from asthmatic patients].
OBJECTIVE: To explore the roles of A2a and A2b adenosine receptors(A2aAR and A2bAR) in the pathogenesis of bronchial asthma. METHODS: Eleven asthmatics and 11 healthy subjects were included in this investigation. Peripheral blood mononuclear cells(PBMCs) were obtained after density centrifugation on Ficoll-Hypaque. The expression of A2aAR and A2bAR mRNAs in PBMCs was examined by use of the reverse transcription-polymerase chain reaction (RT-PCR) and image pattern analysis semiquantitation method. RESULTS: The expression of A2aAR mRNA in PBMCs from asthmatics was not enhanced, compared with that of healthy subjects(P > 0.05). Adenosine or IL-1 or theophylline was not observed to have effect on A2aAR mRNA (P > 0.05). A2bAR gene expression in PBMCs was more markedly in patients with asthma than in normal individuals (P < 0.05). Adenosine and IL-1 significantly increased A2bAR mRNAs in asthmatics. Theophylline inhibited A2bAR gene expressions. The expression of A2bAR mRNA in PBMCs was positively correlated to serum total immunoglobulin E (TIgE) level in asthmatics (r = 0.72, P < 0.01). The expressions of A2bAR mRNA elevated by adenosine or IL-1 were correlated positively with serum TIgE(r = 0.78 or r = 0.61; P < 0.05) and negatively with the forced expiratory volume in first second to predicted value ratio (FEV1%) (r = -0.62 or r = -0.81; P < 0.05) in asthmatics. CONCLUSION: These results indicated that the expression of A2bAR mRNAs in PBMCs was more remarkable in asthmatics than in healthy subjects; that adenosine or IL-1 potentiated the mRNA expression of A2bAR in PBMCs in asthmatic patients, which was correlated with allergy state and the degree of airway obstruction; and that theophylline might antagonize adenosine by inhibiting A2bAR mRNA.
Adenosine↗
[Human papilloma virus infection and expression of p16 protein in laryngeal papilloma and laryngeal carcinoma].
OBJECTIVE: To evaluate the role of human papilloma virus (HPV) infection and inactivation of p16 gene in laryngeal papilloma (LP) and laryngeal squamous cell carcinoma (LC). METHODS: HPV consensus primers direct in situ polymerase chain reaction (ISPCR) and immunohistochemical method were applied to detect the presence of HPV genomes (1, 6, 8, 11, 13, 16, 18, 30, 31, 32, 33, 45, 51) and the expression of p16 protein respectively in 93 cases of formalin-fixed, paraffin-imbedded specimens, which contained 46 cases of LPs [adult-onset laryngeal papilloma (ALP) 21, juvenile-onset laryngeal papilloma (JLP)25], 26 cases of LCs, 6 cases of normal tissues adjacent to carcinoma, and 15 cases of vocal noduli. RESULTS: (1) The difference of positive rates of HPV-DNA in JLP group (84%, 21/25) and other groups were statistically significant (chi 2 test, P < 0.05). The difference of positive rates of HPV-DNA in ALPs(38.1%, 8/21), in LCs(19.2%, 5/26), in vocal noduli(0%, 0/15), and in normal tissues adjacent to carcinoma(0%, 0/6) were not significant statistically (chi 2 test or Fisher's exact probability test, P > 0.05). (2) The positive rates of expression of p16 protein in ALP group(57.1%, 12/21) and LC group(38.5%, 10/26) were significantly lower than that in vocal nodule group(93.3%, 14/15), in JLP group(88%, 22/25), and in normal tissues adjacent to carcinoma group (100%, 6/6) (chi 2 test or Fisher's exact probability test, P > 0.05). There were no significant differences of positive rates of expression of p16 protein between ALP group and LC group, and between JLP group and vocal nodule group (chi 2 test, P > 0.05). (3) In LPs, the difference of positive rates of p16 protein expression between HPV positive cases and HPV negative cases was significant statistically (chi 2 test, P < 0.05). In LCs, there was no difference in p16 protein expression rate between the two teams(Fisher exact probability test, P > 0.05). CONCLUSION: The pathogenesis of JLP is closely associated with HPV infection and not associated with the inactivation of p16 gene. Conversely, the pathogenesis of ALP and LC is associated with the inactivation of p16 gene and not associated with the HPV infection.
Adult↗