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Biomedical subjects

Y Liu

Publications and source records attributed to Y Liu.

At least 685 records · Page 38Linked to original sources
PubMed2001

[Effect of folic acid and supplemented with vitamin A and vitamin E on depressing teratogenesis induced by cyclophosphamide].

The effect of folic acid and the combined effect of folic acid(FA) + vitamin A + vitamin E on the prevention of teratogenesis induced by cyclophosphamide during pregnancy were observed. The results showed that cyclophosphamide resulted a decreased weight and length of the fetuses, an increased rate of neural tube defects and a retardation of ossification centers. The neural tube defects rates in the groups with no any supplementation, supplemented with folic acid alone and supplemented with FA + vitamin A + vitamin E were 87.27%, 42.93% and 30.08% respectively(P < 0.01). The supplementation of FA or the combined supplementation resulted also in greater fetal weight and length, increased ossification of supraoccipital bone (P < 0.01). The study suggested that the combined effect of FA, vitamin A and vitamin E was superior to FA alone.

Animals↗
PubMed2001

[The primary study on rabbit bone marrow mesenchymal stem cells in constructing tissue-engineered cartilage].

OBJECTIVE: To study the feasibility of constructing tissue engineered cartilage by differentiated rabbit bone marrow mesenchymal stem cells(MSC) cultured in vitro and in vivo. METHODS: The MSC were isolated from the nucleated cells fraction of autologous bone marrow by density gradient centrifuge, and then induced into chondrogenic differentiation by adding dexamethasone, transforming growth factor-beta 1 (TGF-beta 1) and ascorbic acid in vitro. After 3 weeks, some cells turned to round shape and secreted metachromatic matrix. The cartilaginoid grafts composed of chondrogenic MSC. Bovine type I collagen and human fibrin were cultured within the chondrogenic medium for 2 weeks in vitro or transplanted subcutaneously adjacent to the knee joint for 3 weeks in vivo. RESULTS: The most cells in the grafts were degenerated and disappeared after cultured in vitro. But the residual cells were survival and secreted metachromatic staining proteoglycan with toluidine blue, which was characteristic cartilage matrix. The grafts developed into matured cartilage tissue assessed by histological examination after 3 weeks of transplantation in vivo. CONCLUSION: MSC can be used as functional cells to constructing tissue engineered cartilage.

Animals↗
PubMed2001

Phacoemulsification in patients with secondary glaucoma caused by uveitis.

PURPOSES: To evaluate the effect of phacoemulsification in the patients with uveitis, secondary glaucoma and complicated cataract. METHODS: Phacoemulsification and implantation of a foldable intraoucular len was performed in 12 patients(13 eyes) with uveitis, secondary glaucoma and complicated cataract. The complications, intraocular pressures (IOP), and visual acuity were observed postoperatively. RESULTS: No severe complication was found in the patients postoperatively or within the operation procedure. The visual acuity was improved after the operation (P < 0.05). The intraocular pressures and/or the number of antiglaucoma medications reduced in 3 months of the follow-up time. CONCLUSION: Phacoemulsification is the best way to treat the patients with secondary glaucoma and complicated cataract caused by uveitis.

Adult↗
PubMed2001

The preliminary clinical observation of array multifocal intraocular lens implantation.

PURPOSE: To evaluate the clinical effects of implantation of Array multifocal intraocular lenses. METHODS: Thirty-one cases (37 eyes) of cataract patients, including 15 males (19 eyes) and 16 females (18 eyes), were involved in this study. All patients underwent standard phacoemulsification with Array multifocal intraocular lens implantation. The complications during operation, postoperative distant visual acuity, near visual acuity, corneal curvature and visual symptoms were observed. RESULTS: The mean value of best postoperative visual acuity was recorded as follows: uncorrected distant visual acuity was 0.8, the best-corrected distant visual acuity was 0.9, uncorrected near visual acuity was 0.5, near visual acuity with distant-corrected was 0.6, the best-corrected near visual acuity was 0.9. The astigmatism of cornea was less than 1.5 D pre-operatively and post-operatively. One patient complained of glare. CONCLUSION: Array multifocal intraocular lens can provide good distant and near visual acuity. With observation of more cases and follow-up of longer time, we can draw a further conclusion.

Aged↗
PubMed2001

[Removal of congenital persistent pupilary membrane with sutureless clear corneal small incision].

PURPOSE: To investigate a new way in the treatment of serious congenital persistent pupilary membrane. METHODS: In four cases of six eyes, after temporal clear corneal tunnel incision was made, viscoelastic material was injected into the anterior chamber, and the persistent pupilary membrane was cut down at the very point connected to the iris. RESULTS: There is no ocular hypertention and hyphema after operation. After one year's follow-up, the lens remained transparent in 3 cases(4 eyes). There was corneal astigmatism of 0.12 to 0.25 D after operation. And only a few corneal endothelial cells were lost. CONCLUSION: There are few postoperative complications and corneal astigmatism to incise congenital persistent pupilary membrane by temporal clear corneal tunnel incision. It is an ideal way to treat serious persistent pupilary membrane.

Anterior Chamber↗
PubMed2001

[The cause and management of acute high intraocular pressure in phacoemulsification].

OBJECTIVE: To investigate the cause and management of acute high intraocular pressure and shallow anterior chamber in phacoemulsification. METHODS: A retrospective study was done on 1,500 eyes performed phacoemulsification. Eleven of 1,500 eyes showed higher intraocular pressure and shallowing of the anterior chamber during phacoemulsification. But phacoemulsification was successfully achieved after giving hyperosmotic agents, increasing infusion pressure and reducing aspiration rate as well as flow rate. RESULTS: Of 11 eyes, 10 eyes were treated by management of non vitrectomy. One of 11 eyes was unresponsive to medical management and require the use of pars plana vitrectomy. Phaco was performed in all patients. In 4 of 11 eyes, visual acuity postoperatively were 0.7 or better. Five of 11 eyes were 0.3 to 0.5. One eye was 0.05 and one was HM/30 cm. CONCLUSION: Aqueous misdirection, retrobulbar hemorrhage and intraoperative suprachoroidal hemorrhage are common causes of acute high intracular pressure in phaco. Most of patients could be performed phaco after medical treatment. If patients failed to improve after medical treatment, par plana vitrectomy is a useful method.

Aged↗
PubMed2001

[Phacoemulsification in patients with cataract and overfiltering bleb after antiglaucoma surgery].

PURPOSE: To evaluate the effect of phacoemulsification in patients with cataract, overfiltering bleb, and low-tension after antiglaucoma surgery. METHODS: Phacoemulsification and implantation of a foldable intraocular len was performed in six patients(7 eyes) with overfiltering bleb, low-tension, and cataract. The complications, blebs, intraocular pressures(IOP), and visual acuity were observed postoperatively. RESULTS: No severe complication was found. The overfiltering blebs constricted and their walls became thicker three months after the operation. The visual acuity and IOP were improved (P < 0.05). CONCLUSION: Phacoemulsification may be another way to treat the patients with cataract and overfiltering bleb, low-tension after antiglaucoma surgery.

Aged↗
PubMed2001

[The inhibitory effect of mitomycin C applied by intracapsular method on rabbit lens epithelial cells: an experimental study of after-cataract].

OBJECTIVE: To study the inhibitory effect of Mitomycin C applied by intracapsular infusion method on rabbit lens epithelial cells and its intraocular toxicity. METHODS: 0.2 ml Mitomycin C with different concentration (0.1 mg/ml, 0.2 mg/ml, 0.4 mg/ml) was injected into the capsular bags of the rabbits' lens during the hydrodissection of phacoemulsification. The drug was exposed to the lens epithelial cells directly and transiently. In the two-month follow-up after the operation, the posterior capsule opacification and the change of intraocular pressure were observed and compared between the control group and the experimental group; the histopathologic and the ultrastructural changes were also observed. RESULTS: For clinical study in the rabbit, the posterior capsule opacification only appeared in the control group two weeks after the operation. Four weeks after the operation, the posterior capsular opacification in the eyes of experimental group was not so obvious as that of the control group. The higher the concentration of the drugs, the less the posterior capsule opacification was shown. There was no obvious difference in the postoperative inflammation between the control group and the low, middle-concentration experimental group. Slight toxic reaction was shown on the rabbits' eyes of the high-concentration experimental group in the early stage after the operation. The histopathologic examination showed that the proliferation of lens epithelial cells in the control group was more obvious than that in the experimental group. Mitomycin C could cause the degeneration of lens epithelial cells. The degree of degeneration was related to the drug's concentration. The ciliary bodies of the rabbits' eyes in the high-concentration experimental group were infiltrated by inflammatory cells and bled slightly. CONCLUSION: Mitomycin C can inhibit the proliferation of lens epithelial cells effectively. Its intensity is related to the drug's concentration. It's a safe and effective drug in the prevention of after-cataract when the concentration of 0.1-0.2 mg/ml was applied in the capsular bag.

Animals↗
PubMed2001

[Experimental pharmacodynamic study on the anti-convulsion effect of shenpu decoction].

OBJECTIVE: To observe the anti-convulsion effect of Shenpu Decoction (SPD). METHODS: Experiments were conducted in three acute convulsion models (cardiazol seizure, strychnine convulsion and maximal electrical shock). Models were divided into the control group (A, treated by normal saline), the high, middle and low dose SPD groups (B, C and D, treated with SPD 9 g/kg, 6 g/kg and 4 g/kg respectively), and the nitrazepam treated group (E). The anti-convulsion effect of SPD was evaluated by 50% convulsion dose (CD50) detected in each group. RESULTS: In cardiazol convulsion model, the CD50 detected in group A-E were 63.3 +/- 3.4 mg/kg, 116.2 +/- 3.4 mg/kg, 105.6 +/- 3.7 mg/kg, 74.0 +/- 3.7 mg/kg and 197.2 +/- 3.3 mg/kg respectively, while they were 0.71 +/- 0.04 mg/kg, 1.21 +/- 0.04 mg/kg, 1.19 +/- 0.04 mg/kg, 0.94 +/- 0.04 mg/kg and 1.16 +/- 0.04 mg/kg respectively in the strychnine convulsion model, and 67.1 +/- 2.6 V, > 140 V, 109.4 +/- 3.5 V, 84.4 +/- 3.1 V and 128.4 +/- 3.9 V in the maximal electrical shock model respectively. CONCLUSION: SPD has a good anticonvulsion effect.

Animals↗
PubMed2001

[Effect of sanwu hypotensive decoction on blood pressure and lymphokine activated killer cell in patient of primary hypertension and spontaneously hypotensive rats].

OBJECTIVE: To investigate the effect of Sanwu hypotensive decoction (SWHD) on blood pressure (BP) and lymphokine activated killer cell (LAK) and possible mechanism. METHODS: Thirty mild hypertension patients were treated with SWHD for 8 weeks, the levels of BP, proliferative ability and activity of LAK cell, SOD-like substance expressed by LAK cell and its radical scavenger ability before and after treatment were observed using randomized, single blinded, self-control, paralled assay, and compared with other 30 patients treated with captopril for control. In an experimental study, the vasodilatory response of thoracic aortic ring to acetylcholine and nitroglycerine, and effect of LAK cell, standard reagent of SOD, SWHD on the response were observed and compared in spontaneously hypertensive rats (SHR) and Wistar Kyoto rats (WKR). RESULTS: After SWHD treatment, along with lowering of BP, the proliferative ability, activity SOD-like substance expressed by LAK cell and its radical scavenger ability increased significantly. CONCLUSION: SWHD is not only an effective hypotensive agent, but also an immunoregulator.

Animals↗
PubMed2001

[Cardiac-hemodynamic effects of M3 receptor agonist on rat and rabbit hearts].

AIM: To study the activation of choline on M3-R in heart and observe the hemodynamic changes of rat and rabbit. METHODS: A cardiac catheter was inserted into the left ventricular cavity via the right carotid artery, then the HR, LVSP, LVEDP, and +/- dp/dt were measured using a polygraph system. RESULTS: Choline was shown to decrease the hemodynamic assessments, such as HR, + dp/dt, LVSP and LVEDP. while the M3-R antagonist 4-DAMP (4-diphenylacetoxy-N-methylpiperidine-methiodide) showed little effect on these assessments. It was found to reverse the hemodynamic effects of choline. CONCLUSION: M3 receptor agonist can produce negative inotropic and chronotropic effects on the heart of rat and rabbit.

Animals↗
PubMed2001

[Interaction between protein and ciprofloxacin].

AIM: To study the interaction between ciprofloxacin and BSA in physiological condition by fluorescence spectroscopy. METHODS: The affection of drug to the protein conformation was investigated. The binding constant between drug and BSA from a double reciprocal Lineweaver-Burk plot was determined and the main sort of binding force was found according to the thermodynamic parameters. RESULTS: The binding constants between BSA and ciprofloxacin at 26 degrees C and 45 degrees C are about 10(4). At 26 degrees C, the thermodynamic parameters of reaction between BSA and ciprofloxacin are delta H = -49.13 kJ.mol-1, delta G = -26.45 kJ.mol-1, delta S = -75 kJ.mol-1. The maximum wavelength of the synchronous fluorescence spectra of BSA moved from 279 nm to 289 nm with the increasing of the amount of ciprofloxacin. CONCLUSION: There exists fluorescence energy transfer between BSA and ciprofloxacin. The main sort of binding force between BSA and ciprofloxacin is Van der Waals' interaction. Ciprofloxacin can be deposited and be transported by serum protein in vivo. Ciprofloxacin affects the protein conformation.

Anti-Infective Agents↗
PubMed2001

[Apolipoprotein C III gene Sst I polymorphism in patients with endogenous hypertriglyceridemia in Chinese population].

OBJECTIVE: To investigate the apoC III gene Sst I polymorphism and its relationship with serum lipids and apolipoproteins (apo) levels in patients with endogenous hypertriglyceridemia in Chinese population. METHODS: The genotype and allele frequency of apoC III gene Sst I polymorphism was assayed by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). Serum lipids were measured by enzymatic kits and apolipoproteins A I, A II, B100, C II, C III and E were measured by RID kits developed by Apolipoprotein Research Unit of WCUMS in 176 HTG patients whose fasting serum TG levels were > or = 2.26 mmol/L and in 199 healthy subjects whose fasting serum TG levels were < 1.82 mmol/L and TC levels < 6.2 mmol/L from a population of Chinese Han nationality in Chengdu area. RESULTS: In both HTG group and control group, S1 allele was the major allele and homozygous S1S1 genotype was the most frequent one. The frequency of S2 allele was significantly higher than that reported in Caucasians (0.289 vs 0.06-0.16, P < 0.05). No differences were found in apoC III gene Sst I polymophism in the HTG group when compared with the control group (0.287 vs 0.289, P > 0.05). The genotype of S2S2 was not associated with higher TG and apolipoproteins levels when compared with the genotypes of S1S1 and S1S2 (P > 0.05). CONCLUSION: These results suggest that the Sst I polymorphism of the apoC III gene was not associated with endogenoushypertriglyceridemica in Chinese population.

Adult↗
PubMed2001

[Studies on the triterpenoidal saponins from flowers of Eriobotrya japonica].

OBJECTIVE: To investigate the chemical composition of the flowers of Eriobotrya japonica. METHODS: Four constituents were isolated from the ethanolic extracts of flowers of Eriobotrya japonica (Thunb) Lindl and were identified by chemical methods and spectroscopic (IR, MS, 1HNMR, 13CNMR). RESULTS: The structures were identified as: Oleanolic acid (I), Ursolic acid(II), 2 alpha, 3 alpha, 19 alpha-Trihydroxyurs-5,12-dien-28-acid (III) and 2 beta, 3 beta, 23 alpha-Trihydroxyolean-12-en-28-acid (IV). CONCLUSION: The compounds I, II, III and IV were obtained from the flowers of Eriobotrya japonica for the first time.

Eriobotrya↗
PubMed2001

[Expression of C-myc and N-myc protein in adulthood and childhood medulloblastomas and prognostic analysis].

OBJECTIVE: To detect the expression of C-myc and N-myc in medulloblastomas, the difference between adults and children, and the relation between expression and prognosis. METHODS: ABC immunostaining was used to detect C-myc and N-myc protein expression in 21 cases of adulthood medulloblastomas and 14 childhood cases. Follow-up materials were obtained to perform survival curve analysis. RESULTS: Of these 35 cases, 5 (4 children, 1 adult) showed C-myc positive expression. No N-myc expression was found. There was no relationship between expression and survival. CONCLUSION: C-myc may play a role in the tumorgenesis of medulloblastoma, especially in children, but it is of no prognostic significance. N-myc antigen may be lost during the fixation of specimens.

Adolescent↗