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Y M Mak

Publications and source records attributed to Y M Mak.

5 recordsLinked to original sources
PubMed1999

Rubisco small subunit gene family in cassava.

Cassava leaves of two different cultivars, Brazil and Buloh, were used to isolate mRNA. The mRNA isolated was successfully used in the construction of cDNA libraries for each of the cultivars. The cDNA libraries were screened for members of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit gene family and positive clones were sequenced. A total of seven different SSU genes, of which five were from cultivar Brazil and two were from cultivar Buloh, were isolated. Comparison results show that even though all the sequences are highly similar, they can be classified into three subfamilies. Homology between members of the same subfamily is higher than homology between members from the same cultivar.

Amino Acid Sequence↗
PubMed1995

Sequence of cassava ribulose-1,5-bisphosphate carboxylase small subunit precursor cDNA.

The cDNA sequence of cassava ribulose-1,5-bisphosphate carboxylase small subunit rbcS precursor has been determined. The cassava rbcS precursor cDNA contains 266 nucleotides (nts) of 5' untranslated region, 546 nts of coding region, 280 nts of 3' untranslated region and a poly (A) tail of 70 nts. The 5' untranslated region is characterized by some unusual features which include two plausible open reading frames, several repeated sequences and a putative Shine-Dalgarno sequence. The predicted amino acid sequence shows that the precursor consists of a 59 amino acid transit peptide followed by the 123 amino acid rbcS sequence. Cassava rbcS shares 82% amino acid sequence identity with a rubber rbcS sequence.

Amino Acid Sequence↗