Biomedical subjects

Y Masatomi

Publications and source records attributed to Y Masatomi.

4 recordsLinked to original sources

PubMed1996

Effects of serum phosphate level on formation of incisor dentine in hypophosphatemic mice.

The effects of serum phosphate level on the formation of incisor dentine were investigated in hypophosphatemic (Hyp) mice fed a diet high in calcium and phosphorus (high Ca/P diet). Feeding a high Ca/P diet for more than 10 days resulted in an increase in the serum phosphate level in Hyp mice to one similar to that of normal mice. Lower incisors were cut transversely at the centre of the length of the incisor, a point that had taken approximately 40 days to be reached from the start of dentine formation in Hyp mice. Transverse views of the incisors showed a triangle-like outline in Hyp mice fed a control diet, while the outline became rounded in Hyp mice fed the high Ca/P diet for more than 40 days. In Hyp mice fed the high Ca/P diet for 40 days interglobular dentine was still observed and fluorescent lines produced by tetracycline showed a diffuse and wavy pattern in incisor dentine; however, interglobular dentine became indistinct and fluorescent lines showed a relatively smooth pattern in the incisor dentine of Hyp mice fed the diet for more than 60 days.

Animals↗

PubMed1992

The occurrence of interglobular dentin in incisors of hypophosphatemic mice fed a high-calcium and high-phosphate diet.

The incisor dentin of hypophosphatemic (Hyp) mice was examined histopathologically to determine whether the multiple occurrences of interglobular dentin would be influenced by the serum phosphate level. Both normal and Hyp mice (12 weeks of age) were divided into two groups. The mice in one group were given a control diet (1.42% Ca, 1.16% P) and the other a high-calcium and high-phosphate diet (2.00% Ca, 3.00% P) for 30 days. Blood was collected from the mice every fifth day for measurement of the calcium and phosphate concentrations in serum. Both ground and decalcified cross-sections were prepared from incisors from the mandible and maxilla for microscopic examination. The levels of serum Ca and P were almost constant in normal mice, regardless of diet. On the other hand, serum P levels in Hyp mice fed the control diet were significantly lower than those in normal mice. The ten days' feeding of the high-Ca/-P diet significantly elevated the serum P level in Hyp mice, and it reached a level similar to that of the normal mice. However, histopathological examination showed no significant changes in incisor dentin of Hyp mice fed the high-Ca/-P diet, and interglobular dentin still occurred. These results suggest that the multiple formations of interglobular dentin, which is the most outstanding feature of X-linked hypophosphatemic vitamin-D-resistant rickets, are not influenced in Hyp mice by the short-time normalization of the serum phosphate level.

Animals↗

PubMed1989

Microscopic and crystallographic examinations of the teeth of the X-linked hypophosphatemic mouse.

Teeth from the C57BL/6J-Hyp mouse (Hyp mouse) were examined histologically, radiographically, and crystallographically. Microscopic examinations of the ground sections, the decalcified and H-E-stained sections, and the contact microradiograms of molars and incisors from the Hyp mouse showed several abnormalities--such as large pulp chamber, wide predentin, thin dentin at the pulp floor, and multiple occurrences of interglobular dentin. Powder and microbeam x-ray diffraction analyses showed that the crystallinity of hydroxyapatite in incisor globular dentin from the Hyp mouse was higher than that in incisor dentin from the normal mouse. On the other hand, the findings of transmission electron microscopy demonstrated that the sizes of hydroxyapatite crystals of globular dentin in the Hyp incisor were larger than those in normal incisor dentin. These results demonstrated that the findings in Hyp mouse teeth were in accord with those of human XLH teeth.

Animals↗

PubMedDate not supplied

Unusual multiple natal teeth: case report.

An 18-month-old Japanese boy with multiple natal teeth was examined. Fourteen hard structures were reported to have been present at birth at the regions of the anterior teeth and first primary molars in both jaws. The structures were excessively mobile and 11 of the structures had exfoliated successively since 5 months of age. The three structures remaining at the regions of the first primary molars had bonelike appearance and color, and were smaller in overall dimension than the corresponding primary teeth. Radiographic examinations showed that the structures had neither roots nor pulp chambers and their radiopacity corresponded to that of mandibular bone. Furthermore, there were no permanent successors except for the upper central incisors and left lateral incisor at the regions where the structures were situated. Histological examinations revealed that the specimens consisted of dentin with tubular structure, osteodentin-like structure, and cementumlike structure.

Anodontia↗