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Biomedical subjects

Y Minesaki

Publications and source records attributed to Y Minesaki.

10 recordsLinked to original sources
PubMed2005

Identification of differentially expressed genes in psoriasis using expression profiling approaches.

To identify differentially expressed genes which play causal roles in pathogenesis and maintenance for psoriasis, we used BodyMapping and introduced amplified fragment length polymorphism approaches. From the BodyMap database, we selected 2007 genes which specifically expressed in epithelial tissues. Among 2007 genes, we surveyed genes which differentially expressed in involved or uninvolved psoriatic lesional skin samples compared with atopic dermatitis, mycosis fungoides, and normal skin samples. As a result of surveying 2007 genes, 241 genes were differentially expressed only in involved psoriatic skin but not in the other samples. Hierarchical cluster analysis of gene expression profiles showed that 13 independent psoriatic-involved skin samples clustered tightly together, reflecting highly similar expression profiles. Using the same 2007 gene set, we examined gene expression levels in five serial lesions from distal uninvolved psoriatic skin to involved psoriatic plaque. We identified seven genes such as alpha-1-microglobulin/bikunin precursor, calnexin, claudin 1, leucine zipper down-regulated in cancer 1, tyrosinase-related protein 1, Yes-associated protein 1, and unc-13-like protein (Coleonyx elegans) which show high-expression levels only in uninvolved psoriatic lesions. These seven genes, which were reported to be related to apoptosis or antiproliferation, might have causal roles in pathophysiology in psoriasis.

Apoptosis↗
PubMed2005

In vitro evaluation of the bonding of auto-polymerizing soft denture liner to cobalt-chromium alloy.

This study evaluated the effects of surface pre-treatments on the bonding of auto-polymerizing silicone soft denture liner to a Co-Cr alloy denture base after cyclic thermal stressing. The bonding surfaces of Co-Cr alloy cylinders (8 mm diameter and 4 mm high) were polished with a 600-grit silicon carbide paper. The bonding surfaces received one of three pre-treatments, which included either the application of a metal primer, a metal primer after air abrasion, or a resin primer after adhesive resin coating. Tensile specimens were fabricated by polymerizing a 2-mm thickness of soft denture liner between a pair of pre-treated denture base cylinders. Failure loads were measured by tensile testing after subjecting the specimen to 0, 5, 10, 20, and 30 thousand thermal cycles. Seven specimens were fabricated for 15 groups, including three pre-treatments and five thermal cycle groups. Failure loads of resin-bonded specimens, which were formerly reported, were used as references. All data were statistically analysed by two-way anova and Bonferroni test at the 95% confidence level. Adhesive resin coating of the metal surface was effective in enhancing the failure loads up to 5000 thermal cycles. However, failure loads of all metal-bonded groups were significantly lower than those of resin-bonded groups at all thermal cycling intervals. The results of this in vitro study implied that polymerizing adhesive resin on the Co-Cr alloy might be a promising method, when the clinicians need to acquire improved bonding of an auto-polymerizing soft denture liner to Co-Cr alloy denture bases.

Air Abrasion, Dental↗
PubMed2003

Bonding abutments to cast metal post/cores: comparison of pre-treatment effects.

Bond strengths were evaluated for (1) metal primer systems when the metal was contaminated by a dentin conditioner and (2) a dentin adhesive system when dentin was contaminated by metal primers. Disc specimens were cast in a silver-palladium-copper-gold (Ag-Pd-Cu-Au) alloy and dentin specimens were prepared by grinding the labial surface of bovine teeth. Specimens were treated with (1) metal primer alone, (2) dentin conditioner alone, (3) metal primer followed by dentin conditioner and (4) dentin conditioner followed by metal primer. A resin cement was poured into a mould over a restricted bonding area and allowed to set. Metal specimens were shear stressed to failure after thermocycling (4-60 degrees C; 20,000 cycles). Dentin specimens were stressed in the same manner after 24 h of immersion in 37 degrees C water. The results were compiled and analysed by anova. Data for dentin specimens treated with dentin conditioner only or with the combination of dentin conditioner and metal primer were not significantly different, statistically. Post-thermocycled groups indicated that bond strengths to the alloy significantly decreased (P < 0.05) when the primed metal surface was contaminated with dentin conditioner regardless of the timing of its application.

Animals↗
PubMed2001

BodyMap incorporated PCR-based expression profiling data and a gene ranking system.

BodyMap is a human and mouse gene expression database that is based on site-directed 3'-expressed sequence tags generated at Osaka University. To date, it contains more than 300 000 tag sequences from 64 human and 39 mouse tissues. For the recent release, the precise anatomical expression patterns for more than half of the human gene entries were generated by introduced amplified fragment length polymorphism (iAFLP), which is a PCR-based high-throughput expression profiling method. The iAFLP data incorporated into BodyMap describe the relative contents of more than 12 000 transcripts across 30 tissue RNAs. In addition, a newly developed gene ranking system helps users obtain lists of genes that have desired expression patterns according to their significance. BodyMap supports complete transfer of unique data sets and provides analysis that is accessible through the WWW at http://bodymap.ims.u-tokyo.ac. jp.

Animals↗
PubMed1999

Paget's disease arising near a male areola without an underlying carcinoma.

We report a 56-year-old man with Paget's disease occurring near the left areola without any underlying breast carcinoma. Histologically, there was no evidence suggesting continuity with a lactiferous duct, accessory breast, or microscopic gynecomastia. We review previous case reports of Paget's disease occurring in unusual sites and discuss their nomenclature from the histogenetic point of view.

Biopsy, Needle↗
PubMed1996

Congenital atrophic plaque.

The patient is a 13-year-old female who had had a patchy lesion on her back since birth which increased in size proportional to her age. When she visited our hospital at the age of 13, the lesion was a 30 x 43 mm, slightly depressed, well defined, irregularly shaped, atrophic plaque. Differential clinical diagnoses at that time included macular atrophy, congenital melanocytic nevus, and mastocytosis. Histopathologically, the lesion was characterized by patchy and scattered infiltrates of oval to spindle cells throughout the dermis extending into the subcutaneous fat tissue. Nerve fibers were found in each of the infiltrates. The tumor cells had oval nuclei with relatively abundant cytoplasm and were similar to nevus cells. They were negative for markers such as S-100 protein, neuron specific enolase, epithelial membrane antigen, HMB-45, Leu 7, Desmin, and alpha smooth muscle actin. However, the histopathological findings suggest that the lesion is a melanocytic or neurogenic tumor, possibly a congenital melanocytic nevus or a cellular neurothekoma, though a definite diagnosis could not be made.

Adolescent↗
PubMed1990

[Thermal properties of human teeth and dental cements].

The thermal properties (thermal diffusivity and thermal conductivity) of human teeth and some dental cements were measured. The temperature change at the pulp cavity was also measured before and after the restoration with the full metal crown. The thermal insulating efficiency of dental cement was discussed. The thermal diffusivity of human dentin, enamel and dental cements was 2.04 x 10(-3), 3.22 x 10(-3), and 0.99-1.87 x 10(-3) cm2/sec, respectively. The thermal conductivity was 1.36 x 10(-3), 1.84 x 10(-3), and 0.50-1.51 x 10(-3) cal/cm.sec.degrees C, respectively. The dental cement beneath the crown insulated the thermal shock to dental pulp and the thermal insulating efficiency was increased with the decrease in the thermal diffusivity and thermal conductivity of dental cement.

Body Temperature↗
PubMed1989

[The effect of the cementing material on the strength of the all ceramic crown].

On the assumption that the all ceramic crown was cemented on an abutment tooth, the effect of the cementing materials on the strength of the ceramic crown was analyzed using two-dimensional finite element technique. The results obtained were as follows: 1. When the compressive load was applied to the ceramics, the principal stress in the cementing material was compressive in the region of Young's modulus of the cementing materials 200-700 kg/mm2, but was in tension beyond it. 2. When the tensile load was applied to the ceramics, the principal stress in the cementing material was in tension, and decreased as Young's modulus of the cementing materials decreased. 3. When the adhesive effect of the cementing material was investigated with examining the presence of the crack between the ceramics and the cementing material, the principal stress in the ceramics increased as the crack propagated. 4. The creation and propagation of the crack, the increase of the principal stress in the ceramics and the destruction of the ceramics were suggested. 5. When the analyzed results were compared with the mechanical properties of the cementing materials from the point of view of the breaking strength, it was considered that the adhesive resin cement was favorable as the cementing material of the ceramic crown. And the Young's modulus of the adhesive resin cement was about 400 kg/mm2.

Crowns↗
PubMed1989

[Effect of the 3/4 crown and porcelain-fused crown restorations on the tooth displacement and the loading force of the supporting tissues].

A mechanical simulation model of tooth and alveolar bone in the application of 3/4 crown and porcelain-fused crown restorations was made and analyzed using three-dimensional finite element technique. The results obtained were as follows. 1. When the physiological tooth mobility was less than 150 microns, the teeth were sufficiently fixed using the splint and the bridge connected with more than two teeth, in the case of 20 percent absorption of the alveolar bone, and more than three teeth in the case of 40 percent absorption. In the case of 60 percent absorption, the teeth were fixed with the splint of six teeth connections, and were fixed with the bridge of five teeth connections. 2. The periodontium supporting force decreased markedly according to the increase of the number of the abutment teeth. 3. The teeth accompanied with the absorption of the alveolar bone were connected, in order to inhibit the tooth mobility and the periodontium supporting force. In this application, it had the inhibitory action when the replacing ratio of metal and ceramics to a crown of the tooth was less than 35 percent. 4. According to the finite element analysis, the design bases of fixed restorations applied in the teeth accompanied with the absorption of the alveolar bone were preferred.

Alveolar Bone Loss↗