[Quality and/or safety of health care].
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Biomedical subjects
Publications and source records attributed to Y Mlynek.
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The immunological methods for quantitating C3d in plasma require first the removal of less fragmented intermediates as well as the intact C3. We describe an alternative method for the quantitation of C3d in human plasma. The components which should be removed are absorbed (precipitated) directly in the plasma by a specific anti-C3c antiserum. It is then possible to determine the concentration of C3d by routine immunological methods.
A patient with an IgG-albumin complex and a gamma heavy chain in his serum was studied by crossed immunoelectrophoresis. The IgG-albumin complex was not dissociated after incubation with 2-mercaptoethanol but it was after incubation with sodium dodecyl sulfate. Evidence was obtained that the IgG and albumin were held together by noncovalent bonds, possibly due to an antigen-antibody reaction.