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Biomedical subjects

Y N Xu

Publications and source records attributed to Y N Xu.

8 recordsLinked to original sources

[Western blotting of RStV gene products in rice and insects].

The NS3 and NC protein genes encoded by RNA3 of RStV, the NCP and NSvc4 protein genes encoded by RNA4 were subcloned into the E. coli expression vector pGEX3X to express four groups of fusion protein under IPTG induction. These fusion proteins were used to immunize rabbits to raise antisera. The antisera against the E. coli-expressed proteins were available for probing the presence of the viral gene products in both rice plant and insect hosts. The expected gene products can be probed only in diseased rice plant with NCP antiserum and the corresponding products detected in both plant and RStV particle preparation with NC antiserum. The viral gene products probed by NS3 and NSvc4 antisera were different from the expected ones in size.

Animals↗

Production of animal model of traumatic pseudoaneurysm.

Three microsurgical techniques, i.e., electric cauterized vein patch (ECVP), electric cauterized vein segment (ECVS), and electric cauterized muscular cavity (ECMC), were employed to produce an ideal animal model of traumatic pseudoaneurysm (TPA). In this paper, 108 cases of TPAs were produced in rabbit femoral arteries. The successful rates of these microsurgical techniques, proved by angiography, autopsy in vivo, and histological examination, were 5.5, 16.7, and 88.9%, respectively. The successful rate of ECMC, with the merits of simple operation, a narrow neck but giant size of TPA which rarely ruptured, and the pulsatile mass occurring as early as 4 to 11 days after operation, was much higher than that of ECVP and ECVS (P < 0.01). By this reliable method of ECMC, the pathogenesis of TPA was observed as the following: the arterial wall was injured and partly ruptured, then the escaped blood was confined by fibrous connective tissue near the arterial opening. As a result, a located hematoma without any pulsatile mass and vascular systolic murmur formed. When a cavity occurred in the hematoma a few days later it gradually enlarged till the clot which plugged the arterial opening became so thin that arterial blood flowed into the cavity to form a pulsating hematoma. So, a pulsatile mass with vascular systolic murmur can be observed.

Aneurysm, False↗

[Hyperbaric oxygen treatment for cystoid macular edema secondary to retinal vein occlusion].

A prospective study was made to estimate the effect of hyperbaric oxygen (HBO) on 14 eyes of cystoid macular edema (CME) secondary to retinal vein occlusion. The visual acuity was improved 2-6 lines (average 3.6 lines) after the treatment, while the control group of 12 cases showed little improvement. The therapeutic effect was stable, however, fluorescein angiography showed no leakage reduction from the perifoveal capillaries, or visual function was enhanced prior to eventual improvement in the fundus. Since HBO therapy has few contraindications nor side effects, it is an effective modality in the treatment of CME.

Aged↗

Arterialized venous free sural nerve grafting.

Since August 1980, a new technique of nerve grafting has been developed in our clinic. A free sural nerve graft, with its attached small saphenous vein arterialized, was used for repairing long segments of nerve defects. Experimental studies of this kind of nerve graft have proved the merits of this new technique. The same favorable results were then obtained in 14 patients, with 2 grafts failing because of vascular thrombosis. We have the impression that the shorter the time interval between the injury and the surgical repair, the better the result. In 2 patients with motor nerve injury, the motor function was well recovered when the operation was performed within 6 months after the injury, whereas none was recovered in those cases with an interval longer than 2 years.

Adult↗