Biomedical subjects

Y Ooba

Publications and source records attributed to Y Ooba.

4 recordsLinked to original sources

PubMed1996

The influence of interleukin-6 on the growth of human esophageal cancer cell lines.

We reported that human esophageal cancer cell lines (ECC) (YES-1, -2, -3, -4, -5, and -6) produced interleukin-6 (IL-6). We, therefore, investigated the growth effects ([3H]thymidine uptake assay and direct cell count) of IL-6 on these ECC. IL-6 receptor (R) and GP-130 mRNA were detected in all the ECC, using reverse transcriptase-polymerase chain reaction (RT-PCR) assay, and IL-6R was detected in one (YES-3) by immunohistochemical staining. IL-6, anti-IL-6 monoclonal antibody (mAb), or anti-IL-6R mAb caused no reproducible enhancement or suppression of [3H]thymidine uptake by all six ECC. Direct cell count also revealed that the growth enhancement or suppression by IL-6, anti-IL-6 mAb, or anti-IL-6R mAb was relatively small. Particularly, there was no significant sensitivity of YES-3 cells, which definitely produce IL-6 and express IL-6R for IL-6, anti-IL-6 mAb, or anti-IL6R mAb. These results suggest that some esophageal cancers may produce IL-6 and express IL-6R. However, no major interactions between IL-6 and the growth of human esophageal cancer cell lines were detected in this study.

Antibodies, Monoclonal↗

PubMed1996

[Optimal protocol for 99mTc-tetrofosmin myocardial SPECT imaging with exercise or dipyridamole stress test and the characteristics of bullseye normal file].

To determine optimal protocol for 99mTc-tetrofosmin SPECT imaging, 30 normal volunteers underwent treadmill or dipyridamole stress test with 770 MBq of tetrofosmin injection. Anterior planar images were acquired every 10-15 minutes for calculating heart/liver ratio. SPECT images were also obtained every 30 minutes for generating Bullseye normal files. In analysis of spatial tracer distribution among normal files, myocardium in Bullseye plot was divided in 9 areas, where mean values of % tracer uptake were calculated and compared by analysis of variance (ANOVA). In those 9 areas, mean values of SD (SD mean) were also compared for predicting artificial blackout in the Bullseye plot among 3 standard normal files: dipyridamole-201Tl, dipyridamole-tetrofosmin (60 min post-injection), and treadmill-tetrofosmin (30 min post-injection). In planar image analysis, high initial uptake in the liver and its acceptable clearance were noted in dipyridamole stress test. Adequate heart/liver ratio was accomplished immediately after treadmill, but 40 minutes later after dipyridamole stress test. ANOVA analysis showed no statistical difference in the spatial % tracer uptake among normal files, irrespective of time after acquisition, data collection time, or difference of tracer. However, SD means in inferior and lateral area of treadmill-tetrofosmin file were significantly small compared to that of dipyridamole-201Tl normal file (p < 0.02 by ANOVA). Thus, we conclude that optimal timing for tetrofosmin SPECT imaging is immediately after treadmill, but at least 40 minutes after dipyridamole stress test. In addition, we should also keep in mind that unexpected blackout may appear in inferior and lateral area, when applying our normal files for reference.

Analysis of Variance↗

PubMed1988

Effects of mannitol in the prevention of evolving myocardial infarction.

The ability of hyperosmolar mannitol to salvage evolving myocardial infarction was studied in 50 open-chest dogs subjected to 3 hours occlusion of the left anterior descending coronary artery. In 28 dogs, 20% hyperosmolar mannitol was infused into the distal half of the infarcted portion after commencement of reperfusion, leaving the proximal half mannitol free. In the other 22 dogs, physiological saline was infused instead of mannitol. The wall thickness at the distal half of the infarcted portion was increased 2 hours after reperfusion in both the saline and the mannitol groups. After 2 hours reperfusion the chest was closed. Thirteen out of 28 dogs in the mannitol group and 12 out of 22 dogs in the saline group died over one week. Seven days later, myocardial samples were measured for myocardial water content and creatine phosphokinase activity (CPK). Myocardial water content was increased in the infarcted portion in both the saline and the mannitol groups. In the saline group, myocardial CPK was reduced markedly in the proximal half (54.8 +/- 7.8% at the inner layer and 53.1 +/- 4.7% at the outer layer). These depletions were even more marked in the distal half (47.9 +/- 5.8 and 48.3 +/- 3.3%). In the mannitol groups, although the CPK was similarly depressed in the proximal half (44.8 +/- 5.8 and 41.1 +/- 5.4%), the CPK depletion in the distal half was less (48.1 +/- 6.1 and 58.1 +/- 6.7%) than the proximal half, suggesting mannitol preserved CPK depletion in the infarcted portion. It was concluded that intracoronary infusion of mannitol after reperfusion may salvage the infarcted myocardium.

Animals↗

PubMed1967

[Magnesium].

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Humans↗