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Biomedical subjects

Y S Chun

Publications and source records attributed to Y S Chun.

43 records · Page 3Linked to original sources
PubMed1994

Eighteen-month evaluation of the effects of a 0.4% stannous fluoride gel on gingivitis in orthodontic patients.

The purpose of this study was to determine whether conventional toothbrushing and twice daily use of a brush-on 0.4% stannous fluoride (SnF2) gel containing more than 90% available Sn2+ would be more effective for controlling plaque accumulation and gingivitis in the presence of orthodontic appliances than conventional toothbrushing alone. Consecutively treated adolescents who were to receive full-mouth fixed orthodontic appliances were assigned to one of two groups according to age and sex criteria. The first group (control, n = 35) used toothbrushing with a standard fluoride toothpaste, whereas the second group (treatment, n = 30) used toothbrushing supplemented with a 0.4% SnF2 gel used twice daily for the entire 18-month study period. Clinical assessments (Plaque Index, Gingival Index, bleeding tendency, and coronal staining) were made single blind before appliances were placed and 1, 3, 6, 9, 12, and 18 months after appliances were placed. Complete data were obtained for 32 control and 23 SnF2 gel subjects. The results indicated that the SnF2 gel group had significantly lower scores for plaque index (p < 0.01), gingival index (p < 0.001), and bleeding tendency (p < 0.001) at all examinations than did the control group. In the SnF2 group, one subject developed mild coronal staining, and two subjects developed moderate staining. We conclude that the use of a 0.4% SnF2 gel containing more than 90% available Sn2+ is an effective adjunct to mechanical tooth cleaning in preventing gingivitis in adolescents undergoing orthodontic treatment with fixed appliances.

Adolescent↗
PubMed1994

Complete nucleotide sequence and tissue-specific expression of the rat phenylethanolamine N-methyltransferase gene.

The rat phenylethanolamine N-methyltransferase (PNMT) gene was isolated from a genomic library by cross-hybridization with a bovine PNMT cDNA probe. Complete nucleotide sequence analysis of a genomic clone showed that this gene contained three exons and spanned about 2.8 kb in length. There were the acute-phase response element, TATA, SP1, and GRE sequences. The physicochemical properties of rat adrenal PNMT were different from those of the brainstem PNMT. However, northern blot and reverse transcription-polymerase chain reaction analysis showed that the rat PNMT gene may not express the multiple forms of mRNA. These results suggest that the rat PNMT gene might produce a single enzyme protein, whose activity may be differentially modulated by tissue-specific environment in the central and peripheral systems.

Adrenal Glands↗
PubMed1993

Identification of PP1 catalytic subunit isotypes PP1 gamma 1, PP1 delta and PP1 alpha in various rat tissues.

cDNA clones for 4 rat protein phosphatase 1 (PP1) catalytic subunit isotypes, PP1 alpha, PP1 gamma 1, PP1 gamma 2 and PP1 delta, were isolated (K. Sasaki et al., Jpn. J. Cancer Res. 81, 1272-1280, 1990). Antibodies were raised to determine the physiological functions of 4 isotypes. Among these isotypes, the PP1 gamma 2 protein was detected specifically in rat testis (H. Shima et al., Adv. Prot. Phosphatases, vol. 7, in press). On the other hand, PP1 gamma 1, PP1 delta and PP1 alpha were detected as 36kDa, 37kDa and 38kDa proteins in several rat organs by immunoblot analysis. All three isotypes were found in both soluble and particulate fractions. PP1 gamma 1 was predominant in the brain and PP1 delta in the brain, lung and small intestine, but the levels of both were low in the liver and muscle. It became evident that PP1 alpha was not a predominant isotype in any tissue examined.

Amino Acid Sequence↗
PubMed1990

Cloning and analysis of the pseudogene for human epinephrine synthesizing enzyme, phenylethanolamine N-methyltransferase (PNMT).

1. This gene completely lacks the intervening sequences. 2. This gene is truncated at the 5' end peptide encoding region by 433 base pairs (bp). 3. The 502 bp of this gene containing poly(A) signal are completely identical to the 3' half of mRNA encoding region of functional gene. 4. This gene has a poly(A) tail and is flanked by direct repeat of 6 bp. 5. Here we report for the first time the complete sequence of a human pseudogene for phenylethanolamine N-methyltransferase and this is the first report of cloning of pseudogene for catecholamine biosynthetic enzymes.

Amino Acid Sequence↗
PubMed1987

Effect of smokeless tobacco on the replication of herpes simplex virus in vitro and on production of viral lesions in hamster cheek pouch.

Previous experiments have shown that combination of herpes simplex virus (HSV) infection and simulated snuff-dipping in hamster buccal pouches enhances the development of micro-invasive squamous cell carcinoma in cheek pouch epithelium. The effect has now been determined of water-extractable components of snuff (snuff-extract) on the growth and the cell-lysing activity of HSV. Various dilutions of snuff-extract in tissue culture medium significantly inhibited the growth of HSV in Vero cell monolayers by inhibiting the viral DNA replication. Moreover, HSV was inactivated and its cell-lysing activity lost when it was incubated with snuff-extract in cell-free condition. Snuff also had a similar anti-herpetic effect in vivo; HSV infection of pouch tissues followed by simulated snuff-dipping resulted in significant inhibition of viral growth. Thus snuff interferes with the DNA synthesis and cytolytic activity of HSV in vitro and in vivo, and this in turn, may increase its oncogenic capacity.

Animals↗
PubMed1987

Effect of ganciclovir [9-(1,3-dihydroxy-2-propoxymethyl)guanine] on viral DNA and protein synthesis in cells infected with herpes simplex virus.

The effect of ganciclovir [9-(1,3-dihydroxy-2-propoxymethyl)guanine] on herpes simplex virus type 1 DNA and protein synthesis was studied. Ganciclovir markedly inhibited the synthesis of viral DNA and gamma proteins in a dose-dependent manner. However, the synthesis of viral beta proteins was significantly increased by ganciclovir in the later stage of infection.

Acyclovir↗