APEC Emerging Infections Network: prospects for comprehensive information sharing on emerging infections within the Asia Pacific Economic Cooperation.
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Asia↗
Biomedical subjects
Y S Lin
Publications and source records attributed to Y S Lin.
In vitro gene transfer in mammalian cells via a new cationic liposome formulation.
A new cationic liposome formulation of sphingosine (SP) and dioleoylphosphatidylethanolamine (DOPE) was developed as an efficient transfection reagent. This SP/DOPE liposome showed efficient transfection in a wide variety of mammalian cancer cells. No significant cytotoxicity of the SP/DOPE liposome to cells was observed. The tranfection activity was greater than that of a well-reported liposome which was made from a cholesterol derivative 3beta-[N-(N',N'-dimethylaminoethane)-carbamoyl] cholesterol (DC-Chol) and the neutral lipid DOPE. In addition, the SP/DOPE liposome was found to be less toxic to cells than the DC-Chol/DOPE liposome. Stable transfections mediated by SP/ DOPE liposome were also demonstrated. These results suggest that the SP/DOPE liposome may provide a good gene delivery system to be used in the human cancer gene therapy.
3T3 Cells↗
Dengue virus infects human endothelial cells and induces IL-6 and IL-8 production.
In this study dengue virus (DV) was found to infect primary endothelial cells derived from human umbilical cord veins (HUVEC) and alter their cytokine production. Dengue virus infection of HUVEC was confirmed by an increase in plaque-forming units in the culture supernatant and by immunofluorescence assay. HUVEC produced large amounts of interleukin (IL)-6 and IL-8 but not IL-1beta after DV infection. Both the replication of DV and the production of IL-6 and IL-8 by HUVEC after DV infection were inhibited by ribavirin, an antiviral synthetic guanosine analogue. Additionally, increased serum levels of IL-6 and IL-8 were observed in patients with dengue hemorrhagic fever but not dengue fever. Therefore, our results suggest that endothelial cells can be a target for DV infection, and that DV-induced IL-6 and IL-8 production by endothelial cells may contribute to the pathogenesis of dengue hemorrhagic fever.
Adolescent↗
[Eventual serologic conversion due to intradermal reaction, in schistosomiasis mansoni].
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Animals↗
Reflex bronchospasm-induced acute massive pulmonary collapse.
Acute massive pulmonary collapse following reflex bronchospasm is described in a patient undergoing general anesthesia. The authors suggest that a chest radiograph should be taken as routine procedure after the onset of airway constriction during anesthesia.
Anesthesia, General↗
Immunostimulation by Alsophila spinulosa extract fraction VII of both humoral and cellular immune responses.
Alsophila spinulosa (Cyatheaceae) has been used in Asia as a herbal medicine. In this study, the immunomodulatory effects of Alsophila spinulosa dried stems water extract fraction VII (AS-VII) were studied in vitro using an animal model. The results showed that AS-VII stimulated splenocyte proliferation in both BALB/c and C3H/HeJ mice. By cell fractionation analysis, the results showed that the mitogenic effect of AS-VII was predominantly on B cell population. The antibody response was also augmented by AS-VII in BALB/c and C3H/HeJ mice. The augmentation of immune responses in C3H/HeJ, the lipopolysaccharide (LPS)-hyporesponsive mouse strain, indicated that the activity was not simply due to the LPS contamination which may be present in AS-VII preparation. Furthermore, AS-VII caused an increase in cytotoxic T cell activity in a mixed lymphocyte reaction culture. These data indicate that AS-VII possesses the capability of augmenting both humoral and cellular immune responses.
Adjuvants, Immunologic↗
Lack of evidence for the role of staphylococcal enterotoxins in rheumatoid arthritis.
OBJECTIVE: The etiology of rheumatoid arthritis (RA) is not clear. Evidence of limited usage of T cell receptor V beta genes has been found in rheumatoid synovium. Therefore, a pathological mechanism in which superantigens (SAgs) activate T cells with particular T cell receptor V beta chains may exist. However, no direct functional studies of this possible mechanism have yet been reported. In this study we investigated the direct functional responses of lymphocytes from patients with RA to different bacterial superantigens. METHODS: A primary lymphocyte proliferation test to four different staphylococcal enterotoxins (SEs) was performed in 36 patients with RA to gather information about the relationship between SAg and RA. RESULTS: The results showed that patients with RA had a decreased proliferative response to all four SEs compared with normal subjects. The decreased response was also found in Con A stimulation. This decreased proliferative response to SEs was not unique to RA patients, but was also observed in patients with systemic lupus erythematosus. CONCLUSION: We hypothesize that this decreased response to SEs only reflects the generalized immunodeficient status of patients with RA. The results do not suggest that any of the four SEs are involved in the pathogenesis of RA.
Arthritis, Rheumatoid↗